86 Characterization and differentiation of binding modes of water-soluble porphyrins at complexation with DNA

131 Polymorphism within complex DNA topologies

“…The inspection of obtained parameters n provides additional evidences for the proposed models: for H 2 TOEPyP4 n~1.8, which correspond to intercalation mode, and n~.5-1 for CuTOEPyP4 and CoTOEPyP4, which is sufficient for groove binding mode (Barkhudaryan, Ananyan, & Dalyan, 2013;Barkhudaryan, Ananyan, Dalyan, & Haroutiunian, 2014). This research has been supported by grants SCS of MES of RA 13-1F094.…”

135 The comparative study of H2TOEPyP4 and CoTOEPyP4 porphyrins with A-DNA

“…All studies were performed in diluted solution of BPSE buffer (BPSE = 6 mM Na 2 HPO 4 + 2 mM NaH 2 PO 4 + 185 mM NaCl + 0.1 mM EDTA), pH 7.0, ionic strength [Na + ] = 10 −3 M. The results indicated that the presence of Co in porphyrin ring was decreasing binding constant 10 times as compared with H 2 TOEPyP4 porphyrin. The induced CD spectra was shown (Pasternack, 2003), that H 2 TOEPyP4 and CoTOEPyP4 porphyrins interact with A-DNA only through external groove binding, while H 2 TOEPyP4 interact with B-DNA by intercalative mode at small porphyrin-DNA ratio (Barkhudaryan, Ananyan, & Dalyan, 2013), which consequently changes into outside stacking mode at higher rations (Avetisyan, 2014). From the dependencies of binding constants (ln K b ) versus 1/T we evaluated the free binding energies of complexation (ΔG b ), enthalpies (ΔH b ) and entropies (ΔS b ).…”

134 Large antiviral polyamide-DNA interactions

“…All studies were performed in diluted solution of BPSE buffer (BPSE = 6 mM Na 2 HPO 4 + 2 mM NaH 2 PO 4 + 185 mM NaCl + 0.1 mM EDTA), pH 7.0, ionic strength [Na + ] = 10 −3 M. The results indicated that the presence of Co in porphyrin ring was decreasing binding constant 10 times as compared with H 2 TOEPyP4 porphyrin. The induced CD spectra was shown (Pasternack, 2003), that H 2 TOEPyP4 and CoTOEPyP4 porphyrins interact with A-DNA only through external groove binding, while H 2 TOEPyP4 interact with B-DNA by intercalative mode at small porphyrin-DNA ratio (Barkhudaryan, Ananyan, & Dalyan, 2013), which consequently changes into outside stacking mode at higher rations (Avetisyan, 2014). From the dependencies of binding constants (ln K b ) versus 1/T we evaluated the free binding energies of complexation (ΔG b ), enthalpies (ΔH b ) and entropies (ΔS b ).…”