8-Oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2′-deoxyguanosine levels in human urine do not depend on diet

Gackowski, Daniel; Różalski, Rafał; Roszkowski, Krzysztof; Jawień, Arkadiusz; Foksiński, Marek; Oliński, Ryszard

doi:10.1080/10715760100301321

Cited by 94 publications

(69 citation statements)

References 29 publications

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“…Other factors, such as cell death and diets, might contribute to increase urinary 8-oxodG levels. However, the evidence was limited (36,37). Although genetic background (such as DNA repair capability) from each participant could confound the estimated effect, two measurements on the consecutive days in the present study could minimize these effects.…”

Section: Discussionmentioning

confidence: 83%

Exposure to Heavy Metals and Polycyclic Aromatic Hydrocarbons and DNA Damage in Taiwanese Traffic Conductors

Huang

Chen

Wang

et al. 2013

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background: Exposure to traffic-related air pollutants, including polycyclic aromatic hydrocarbons (PAH) and heavy metals, has been associated with the etiology and prognosis of many illnesses. However, the specific causal agents and underlying mechanisms for different health outcomes remain unclear. The aims of this study were to assess the relations between urinary biomarkers of exposure to PAHs (1-hydroxypyrene-glucuronide, 1-OHPG) and heavy metals (cadmium, Cd; nickel, Ni; arsenic, As; lead, Pb; and copper, Cu) and the effect of their interaction on DNA damage (8-oxo-7,8-dihydro-guanine, 8-oxodG).Methods: We recruited 91 traffic conductors and 53 indoor office workers between May 2009 and June 2011 in Taipei, Taiwan. Postshift urine samples from 2 consecutive days were analyzed for 1-OHPG, Cd, Ni, As, Pb, Cu, and 8-oxodG. To estimate the effects from PAHs and metals on DNA damage, we constructed a linear mixed model adjusted for confounding variables.Results: We found that urinary 1-OHPG and Cd levels were independent predictors of urinary 8-oxodG levels (b ¼ 0.112; P ¼ 0.015 for 1-OHPG; b ¼ 0.138; P ¼ 0.031 for urinary Cd). The joint effect of urinary 1-OHPG and Cd levels was associated with urinary 8-oxodG levels (P ¼ 0.001).Conclusions: Co-exposure to environmental PAHs and Cd could cause oxidative DNA damage. Impact: These findings suggest that the additive interaction between exposure to environmental PAHs and Cd could enhance the burden of oxidative stress. Cancer Epidemiol Biomarkers Prev; 22(1); 102-8. Ó2012 AACR.

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Section: Discussionmentioning

confidence: 83%

Exposure to Heavy Metals and Polycyclic Aromatic Hydrocarbons and DNA Damage in Taiwanese Traffic Conductors

Huang

Chen

Wang

et al. 2013

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…As with previous studies (75), the authors could only presume that the isotopically labeled DNA components/adducts pass through the gastrointestinal tract to appear in feces. Nevertheless, these results, coupled with the findings of Gackowski et al (87), provide the most compelling argument that diet is not a significant contributor to both urinary 8-oxoGua and 8-oxodG levels in human urine. This is consistent with the animal data relating to 2 ¶-deoxyribonucleoside lesions (75), which includes thymidine glycol (23), but disagrees with early data for 8-oxoGua and thymine glycol (23,89).…”

Section: Sources Of Extracellular Oxidatively Modified Dna Lesionsmentioning

confidence: 79%

Measurement and Meaning of Oxidatively Modified DNA Lesions in Urine

Cooke

Oliński²,

Loft³

2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

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205

147

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Background: Oxidatively generated damage to DNA has been implicated in the pathogenesis of a wide variety of diseases. The noninvasive assessment of such damage, i.e., in urine, and application to large-scale human studies are vital to understanding this role and devising intervention strategies. Methods: We have reviewed the literature to establish the status quo with regard to the methods and meaning of measuring DNA oxidation products in urine. Results: Most of the literature focus upon 8-oxo-7,8-dihydro-2 ¶-deoxyguanosine (8-oxodG), and whereas a large number of these reports concern clinical conditions, there remains (a) lack of consensus between methods, (b) possible contribution from diet and/or cell death, (c) no definitive DNA repair source of urinary 2 ¶-deoxyribonucleoside lesions, and (d) no reference ranges for healthy or diseased individuals. Conclusions: The origin of 8-oxodG is not identified; however, recent cell culture studies suggest that the

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“…Reardon et al (28) showed the removal of 8-oxodG by NER. A recent study suggests that NER represents a plausible mechanism by which 8-oxodG and oligomers containing 8-oxodG appear extracellularly in cell culture supernatant, plasma, or urine (29). XPA was previously reported to be actively involved in the dual incision step of repairing ROS-induced 5V,8-purine cyclodeoxynucleosides, which is formed by intramolecular cross-linking between the C-8 position of adenine or guanine and the 5V position of 2-deoxyribose (30).…”

Section: Discussionmentioning

confidence: 99%

Nucleotide Excision Repair Gene Polymorphisms and Recurrence after Treatment for Superficial Bladder Cancer

Gu¹,

Zhao²,

Dinney

et al. 2005

Clinical Cancer Research

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Purpose: Interindividual differences in DNA repair capacity not only modify individual susceptibility to carcinogenesis, but also affect individual response to cancer treatment. Nucleotide excision repair (NER) is one of the major DNA repair pathways in mammalian cells involved in the removal of a wide variety of DNA lesions. Polymorphisms in NER genes may influence DNA repair capacity and affect clinical outcome of bladder cancer treatment. Experimental Design: To test the influence of NER gene polymorphisms on superficial bladder cancer outcome (recurrence and progression), we conducted a follow-up study of 288 patients with superficial bladder cancer. Median follow-up among patients who were recurrence-free at the end of observation was 21.7 months from diagnosis. The specific polymorphic loci examined include XPA [A/G at 5′ untranslated region (UTR)], XPC (poly AT, Ala499Val, Lys939Gln), XPD (Asp312Asn, Lys751Gln), XPG (His1104Asp), ERCC 1 (G/T at 3′ UTR), and ERCC6 (Met1097Val, Arg1230Pro). Results: The ERCC6 (Met1097Val) polymorphism had a significant impact on recurrence: carriers of at least one variant allele (Val) had a significantly higher recurrence risk than carriers of the wild-type allele (Met/Met; hazard ratio, 1.54; 95% confidence interval, 1.02-2.33). There were no overall statistically significant differences in the distributions of the other polymorphisms between patients with and without recurrence. However, when we combined these variant genotypes, there was a significant trend for an increased recurrence risk with an increasing number of putative high-risk alleles. Using individuals with five or fewer putative high-risk alleles as the reference group, individuals with six to seven risk alleles and individuals with eight or more risk alleles had higher recurrence risks, with hazard ratios of 0.92 (0.54-1.57) and 2.53 (1.48-4.30), respectively (P for trend < 0.001). There was also a significant trend for shorter recurrence-free survival time with increasing number of variant alleles (log rank test, P = 0.0007). When we stratified the patients according to intravesical Bacillus Calmette-Guerin treatment, we found a significant trend for shorter recurrence-free survival time in patients with variant alleles of XPA or ERCC6 polymorphisms who received Bacillus Calmette-Guerin treatment (log rank test, P = 0.078 and 0.022, respectively). There were no significant individual or joint associations between these polymorphisms and progression. Conclusions: These data suggest that interindividual differences in DNA repair capacity may have an important impact on superficial bladder cancer recurrence. A pathway-based approach is preferred to study the effects of individual polymorphism on clinical outcomes.

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8-Oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2′-deoxyguanosine levels in human urine do not depend on diet

Cited by 94 publications

References 29 publications

Exposure to Heavy Metals and Polycyclic Aromatic Hydrocarbons and DNA Damage in Taiwanese Traffic Conductors

Exposure to Heavy Metals and Polycyclic Aromatic Hydrocarbons and DNA Damage in Taiwanese Traffic Conductors

Measurement and Meaning of Oxidatively Modified DNA Lesions in Urine

Nucleotide Excision Repair Gene Polymorphisms and Recurrence after Treatment for Superficial Bladder Cancer

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