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Pires, Andrea; Andreiuolo, Felipe; Souza, Simone Rabello de

doi:10.1186/1746-1596-1-14

Cited by 56 publications

(20 citation statements)

References 9 publications

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“…35 Following the construction of the array blocks, 4 μm sections were cut with a microtome and placed on silane-coated slides for immunohistochemical analysis following published procedures. 26-29 Briefly, the 4 μm paraffin-embedded sections were dehydrated, incubated in 3% hydrogen peroxide for 10 min, and incubated in trypsin for 20 min.…”

Section: Methodsmentioning

confidence: 99%

Secretory Leukocyte Protease Inhibitor Expression and High-Risk HPV Infection in Anal Lesions of HIV-Positive Patients

Nicol

Brunette

Nuovo

et al. 2016

JAIDS Journal of Acquired Immune Deficiency Syndromes

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Objective The aim of the current study was to evaluate secretory leukocyte protease inhibitor (SLPI) expression in anal biopsies from HIV-positive (HIV+) individuals, and compare that to anal intraepithelial neoplasia (AIN) diagnoses and human papillomavirus (HPV) status. Design This is a cross-sectional study of a cohort of 54 HIV+ (31 males and 23 females) from an AIDS clinic in Rio de Janeiro, Brazil. Methods The study material consisted of anorectal tissue biopsies obtained from HIV+ subjects, which were used to construct tissue microarray paraffin blocks for immunohistochemical analysis of SLPI expression. Biopsies were evaluated by an expert pathologist and classified as low-grade anal intraepithelial neoplasia (AIN1), high-grade anal intraepithelial neoplasia (AIN2/3), or normal squamous epithelium. Additionally, DNA from the biopsies was extracted and analyzed for the presence of low- or high-risk HPV DNA. Results Histologically normal squamous epithelium from the anorectal region showed strong positive SLPI staining in 17/20 (85%) samples. In comparison, 9/17 (53%) dysplastic squamous epithelial samples from AIN1 patients showed strong SLPI staining, and only 5/17 (29%) samples from AIN2-3 patients exhibited strong SPLI staining, which both were significantly fewer than those from normal tissue (p=0.005). Furthermore, there was a significantly higher proportion of samples in which oncogenic high-risk HPV genotypes were detected in low SLPI expressing tissues than that in tissues with high SLPI expression (p=0.040). Conclusion Taken together these results suggest that low SLPI expression is associated with high-risk HPV infections in the development of AIN.

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Section: Methodsmentioning

confidence: 99%

Secretory Leukocyte Protease Inhibitor Expression and High-Risk HPV Infection in Anal Lesions of HIV-Positive Patients

Nicol

Brunette

Nuovo

et al. 2016

JAIDS Journal of Acquired Immune Deficiency Syndromes

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“…Among these specimens, 31 were cervical samples from HIV/HPV coinfected patients. The TMA blocks were constructed as previously described by Pires et al (2006). The punches were 1 mm in diameter and consisted of two cores with full thickness of the cervical epithelium.…”

Section: Subjects Materials and Methodsmentioning

confidence: 99%

An evaluation of p16INK4a expression in cervical intraepithelial neoplasia specimens, including women with HIV-1

Nicol

Golub

Silva

et al. 2012

Mem. Inst. Oswaldo Cruz

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Although several studies have evaluated the role of p16INK4a as a diagnostic marker of cervical intraepithelial neoplasia (CIN) and its association with disease progression, studies regarding the role of p16INK4a in human immunodeficiency virus (HIV)-infected patients remain scarce. The present study was designed to determine the potential utility of p16INK4a as a diagnostic marker for CIN and invasive cervical cancer in HIV-positive and negative cervical specimens. An immunohistochemical analysis of p16INK4a was performed in 326 cervical tissue microarray specimens. Performance indicators were calculated and compared using receiving operating characteristics curve (ROC)/area under the curve. In HIV-1-negative women, the percentage of cells that was positive for p16INK4a expression was significantly correlated with the severity of CIN (p < 0.0001). A ROC curve with a cut-off value of 55.28% resulted in a sensitivity of 89%, a specificity of 81%, a positive predictive value of 91% and a negative predictive value of 78%. HIV-seropositive women exhibited decreased expression of p16INK4a in CIN2–3 specimens compared with HIV-negative specimens (p = 0.031). The ROC data underscore the potential utility of p16INK4a under defined conditions as a diagnostic marker for CIN 2–3 staging and invasive cervical cancer. HIV-1 infection, however, is associated with relatively reduced p16INK4a expression in CIN 2–3.

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“…The high cost of the array machines limits its use in general practice in many countries [6]. Efforts have been made by many researchers to devise more cost effective TMA construction techniques [2,7-11]. Some of those researchers used bone marrow trephine biopsy needles or other types of needles, however our mechanical pencil tip method have proven to be much more cheap and easy.…”

Section: Discussionmentioning

confidence: 99%

An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips

et al. 2011

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BackgroundTissue microarray technology has provided a high throughput means of evaluating potential biomarkers in archival pathological specimens. This study was carried out in order to produce tissue microarray blocks using mechanical pencil tips without high cost.MethodConventional mechanical pencil tips (Rotring Tikky II Mechanical Pencil 1.0 mm) were used to cut out 1 mm wax cylinders from the recipient block, creating from 36 to 72 holes. Three cores of tumor areas were punched out manually by using the mechanical pencil tips from donor paraffin embedded tissue blocks and transferred to the holes of the paraffin tissue microarrays.ResultsThis technique was easy and caused little damage to the donor blocks. We successfully performed H&E slides and immunodetection without substantial tissue cylinder loss.ConclusionOur mechanical pencil tip technique is the most inexpensive easy technique among the literature. It also takes a reasonable amount of time and reduces antibody consumption during immunohistochemistry

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Cited by 56 publications

References 9 publications

Secretory Leukocyte Protease Inhibitor Expression and High-Risk HPV Infection in Anal Lesions of HIV-Positive Patients

Secretory Leukocyte Protease Inhibitor Expression and High-Risk HPV Infection in Anal Lesions of HIV-Positive Patients

An evaluation of p16INK4a expression in cervical intraepithelial neoplasia specimens, including women with HIV-1

An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips

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