Nicole Rachfall, Isabelle Heinemeyer, Burkhard Morgenstern, Oliver Valerius, Gerhard H. Braus
We describe a method to identify and analyze translationally regulative 5UTRs (5TRU) in Saccharomyces cerevisiae. Two-dimensional analyses of 35 S-methionine metabolically labeled cells revealed 13 genes and proteins, whose protein biosynthesis is post-transcriptionally up-regulated on amino acid starvation. The 5UTRs of the respective mRNAs were further investigated. A plasmidbased reporter-testing system was developed to analyze their capability to influence translation dependent on amino acid availability.…
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