5-Hydroxytryptamine and the Skin

West, G. B.; Parratt, J. R.

doi:10.1001/archderm.1957.01550210060007

Cited by 41 publications

(6 citation statements)

References 4 publications

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“…They also showed that 2 days after weaning, the histamine and 5-HT levels were twice those found in unweaned littermates and the mast cells were not increased in number. They maintain that the abundant 5-HT which is found in the outer layer of the rat skin is not held in mast cells (6). In the present experiment, however, we could not demonstrate the presence of 5-HT outside mast cells with the histochemical method.…”

Section: Treatment With 5-ht or 5-htpcontrasting

confidence: 92%

“…The occurrence of 5-hydroxytryptamine (5-HT) in the mast cells of the rat skin has been demonstrated by many investigators using a biochemical method (1)(2)(3)(4)(5)(6)(7). However, Parratt and West (7) have reported that there is no correlation between the distribution of mast cells and the level of 5-HT in different layers of rat skin.…”

mentioning

confidence: 99%

“…According to them, the level of 5-HT in the abdominal epithelium is higher than that in the subcutaneous tissue which is reported to contain a larger amount of mast cells and endogenous histamine. They have concluded that most part of 5-HT in the rat skin is present outside of mast cells (6).…”

mentioning

confidence: 99%

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The Histoghemigal Demonstration of 5-Hydroxy-Tryptamine-Containing Mast Cell in Rat Skin

Fujii

Imamura

Suzuki

1968

Japanese Journal of Pharmacology

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The occurrence of 5-hydroxytryptamine (5-HT) in the mast cells of the rat skin has been demonstrated by many investigators using a biochemical method (1-7). However, Parratt and West (7) have reported that there is no correlation between the distribution of mast cells and the level of 5-HT in different layers of rat skin. According to them, the level of 5-HT in the abdominal epithelium is higher than that in the subcutaneous tissue which is reported to contain a larger amount of mast cells and endogenous histamine. They have concluded that most part of 5-HT in the rat skin is present outside of mast cells (6).Recently, a histochemical method for demonstrating the endogenous 5-HT in differ ent tissues has been developed by Scandinavian investigators (8-11). The present experi ment is an attempt to show the distribution of 5-HT in the morphological components of various parts of rat skin using this technique. METHODSFemale Wistar rats weighing 100-150 g were used. Preparation of tissue section for fluorescence histochemistryThe parts of skin for histochemical study were shaved on the day before experiment.The animals were exsanguinated after killing and the parts of skin were removed, then the pieces of 2 x 4 mm were prepared. The corresponding parts were taken at least from 2 animals. The pieces were frozen in a mixture of acetone and dry ice at a temperature of -80,c and then freeze-dried in vacuo at -30' to -40'C for 6-8 days. The freeze-dried tissues were exposed to formaldehyde vapor from paraformaldehyde for 60 minutes at 80°C. The tissues exposed to formaldehyde vapor were infiltrated in vacuo with liquid paraffin (m.p. 46-48°C) for 24 hours and thereafter embedded with solid paraffin (m.p. 56-58°C). Sections (6-8,) were prepared under low humidity, then placed on nonfluorescent slide glasses. After deparaffinization with xylene the sections were mounted in liquid paraffin. A fluorescence microscope (Carl-Zeiss) with activating lamp of Osram HBO-200 was used for microscopic observations. The primary filter was a Schott BG-12 and the second

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Section: Treatment With 5-ht or 5-htpcontrasting

confidence: 92%

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confidence: 99%

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The Histoghemigal Demonstration of 5-Hydroxy-Tryptamine-Containing Mast Cell in Rat Skin

Fujii

Imamura

Suzuki

1968

Japanese Journal of Pharmacology

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“…thus Feldberg and Smith (1953) showed that tryptamine and 5-hydroxytryptamine can act as histamine-liberators in vivo. One might speculate that the traces of 5-HT which are normally found in the mast cells of the mouse and rat (Benditt et al, 1955;West and Parratt, 1957) have reached the cells in this way.…”

Section: Table XXmentioning

confidence: 97%

The mast cells

Riley¹

1959

252

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'coarse granulocyte' in echinoderms is first recognizable as a connective tissue cell with clear cytoplasm, and thus corresponds to the 'hyaline cell' of Kollmann (1908). Under conditions of good feeding the cell develops bulky refractile spherules which stain with basic dyes. The spherules then progressively become yellow and shrunken; finally often red and acidophilic. Thus the intermediate coarse granulocyte in invertebrates, the cell with colourless, basophilic spherules, bears the most obvious resemblance to the mast cell of higher animals. Kollmann found these cells in the connective tissues of various invertebrate forms which he examined. 'Les cellules spheruleuses. .. restent habituellement cantonnees dans le tissu conjonctif. On peut vraisemblement les comparer aux Mastzellen des Vertebres' (Kollmann, p. 200). Thus it will be observed from the standpoint of comparative morphology that mast cells are regularly demonstrable before the demand for oxygen and nutriment has led to the evolution of a definitive blood-vascular system. Whatever may be the function of the mast cells in these simple organisms it can hardly be concerned with the blood. The common blood cell of many insects appears to be concerned function-* Ehrich's 'Mastzellen' are variously described by later investigators as 'cellules isoplastiques Adrenals, mast cells in, 22 Alimentary tract, lower vertebrates, mast cells in, 9 Amino acids, basic, in mast cell, 125, 126, 162 Amoebocyte with spherules, 6 Amphibia, blood formation in, 7 mast cells in, 7

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“…8 Serotonin has been found in all of the phyla except the coelenterates. It has been suggested by Collier 9 that this widespread distribution indicates one of two things : Either the capacity to produce this sub¬ stance is easily developed as occasion demands or the ability of production is primitive in evolution.…”

Section: Introductionmentioning

confidence: 99%

Serotonin

Clendenning¹

1959

AMA Arch Derm

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5-Hydroxytryptamine and the Skin

Cited by 41 publications

References 4 publications

The Histoghemigal Demonstration of 5-Hydroxy-Tryptamine-Containing Mast Cell in Rat Skin

The Histoghemigal Demonstration of 5-Hydroxy-Tryptamine-Containing Mast Cell in Rat Skin

The mast cells

Serotonin

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