5-hydroxytryptamine and glutamate modulate velocity and extent of intercellular calcium signalling in hippocampal astroglial cells in primary cultures

Blomstrand, Fredrik; Khatibi, Siamak; Muyderman, Håkan; Hanssoń, Elisabeth; Olsson, Torsten; Rönnbäck, Lars

doi:10.1016/s0306-4522(98)00351-0

Cited by 59 publications

(41 citation statements)

References 55 publications

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“…In addition, serotonin may move through gap junctions to coordinate the left-right body axis and modulate intercellular calcium signaling, and ADF neurons of the NSY-5 network synthesize serotonin (Blomstrand et al, 1999;Duerr et al, 1999;…”

Section: Ip3 and Serotonin Signaling Are Not Required For Awc Asymmetrymentioning

confidence: 99%

Intercellular calcium signaling in a gap junction-coupled cell network establishes asymmetric neuronal fates in C. elegans

et al. 2012

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Section: Ip3 and Serotonin Signaling Are Not Required For Awc Asymmetrymentioning

confidence: 99%

Intercellular calcium signaling in a gap junction-coupled cell network establishes asymmetric neuronal fates in C. elegans

et al. 2012

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“…Gap junction channels connect the cytoplasm of adjacent cells, and are crucial for synchronization of astrocytic signaling, calcium wave propagation, and spatial buffering of potassium (Blomstrand et al, 1999a;Cotrina et al, 1998;Giaume and Venance, 1998;Wallraff et al, 2006). Astrocyte gap junction coupling can be modulated by various neuroactive substances, suggesting that channels open and close in response to neuronal activity (Blomstrand et al, 1999b;Blomstrand et al, 2004;Enkvist and McCarthy, 1994;Rouach et al, 2000;Venance et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Electrophysiological properties and gap junction coupling of striatal astrocytes

Adermark

Lovinger

2008

Neurochemistry International

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The striatum is the biggest nucleus of the basal ganglia and receives input from almost all cortical regions, substantia nigra and the thalamus. Striatal neuronal circuitry is well characterized, but less is known about glial physiology. To this end, we evaluated astrocyte electrophysiological properties using whole-cell patch-clamp recording in dorsal striatal brain slices from P15 -P21 rat. The majority of cells (95%) were passive astrocytes that do not express any detectable voltage-gated channels. Passive astrocytes were subcategorized into three groups based on time dependent current properties. The observed proportion of the different astrocyte subtypes did not change within the age range evaluated here, but was modulated during reduction of specific conductances and gap junction coupling. Striatal astrocytes were extensively interconnected and closure of gap junctions with octanol (1 mM), carbenoxolone (100 µM) or increased intracellular calcium (2 mM), significantly altered intrinsic properties. When simultaneously blocking potassium channels and gap junction coupling almost no passive conductance was detected, implying that the major currents in striatal astrocytes derive from potassium and gap junction conductance. Uncoupling of the syncytium reduced currents activated in response to a hyperpolarizing pulse, suggesting that changes in gap junction coupling alters astrocyte electrophysiological responses. Our findings indicate that the prevalent gap junction coupling is vital for astrocyte function in the striatum, and that whole cell recordings will be distorted by currents activated in neighboring cells.

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“…The capillary endothelial cells release substances that influence the astrocyte endfeet, and are thereby influenced by the cytokines interleukins, transforming growth factor β (TGFβ), tumor necrosis factor-α (TNF-α) and inflammatory substances; proteases, tryptases, chymases, and matrix metalloproteinases (MMPs or matrixins) within the CNS [25]. Ca 2+ -mediated signaling in astrocytes and propagation of Ca 2+ fluctuates between astrocytes via gap junctions, where connexin 43 (Cx43), and ATP and its purinergic receptors play important roles [26][27][28]. Via intercellular communication astrocytes act as a syncytium for modulating neuronal and vascular function.…”

Section: Syncytium Coupled Cellsmentioning

confidence: 99%

“…The spread of Ca 2+ waves through the astrocyte syncytium has a propagating rate of about 100 µm/s [27,54]. Astrocytes in most part of the nervous system use two types of Ca [27], and at disturbed properties extracellular communication through enhanced release of ATP. Astrocytic Ca 2+ waves are mediated by autocrine activation of purinoceptors [26].…”

Section: Down Regulation Of Signaling Systems In Syncytium Coupled Cellsmentioning

confidence: 99%

Gap Junction Coupled Cells, Barriers and Systemic Inflammation

Rönnbäck¹

2017

IJOAO

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The blood-brain barrier (BBB), the blood-retinal barrier (BRB), and the blood-nerve barrier (BNB) may have similarities in systemic chronic inflammation, as seen in a variety of diseases. A disturbance of the barriers lead to a disruption of the cells coupled with gap junctions in syncytium. The network coupling may be principal to understand the homeostatic imbalance in systemic inflammation. This review high-lightens the role of gap junction network coupled cells in inducing and maintaining barrier properties under physiological conditions as well as their involvement in inflammatory pathologies. Rigshospitalet, 2600 Glostrup, Denmark, Tel. +45 3863 4820, Fax +45 3863 4834; E-mail elisabeth.cecilia.roennbaeck@regionh.dk and waste products between the vascular lumen and the neural retina and is formed by the interaction of retinal glia and pericytes with the endothelium. The blood-nerve barrier (BNB) consists of blood vessels and sensory nerves but gap junction coupled cells in networks, such as the articular cartilage in joints, might be involved in these cell interactions [3]. KeywordsFuture studies elucidating barrier induction and maintenance will provide a framework for rational therapies in several diseases, to restore the barriers with influence of gap junction syncytium coupled cells on the barrier functions.

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5-hydroxytryptamine and glutamate modulate velocity and extent of intercellular calcium signalling in hippocampal astroglial cells in primary cultures

Cited by 59 publications

References 55 publications

Intercellular calcium signaling in a gap junction-coupled cell network establishes asymmetric neuronal fates in C. elegans

Intercellular calcium signaling in a gap junction-coupled cell network establishes asymmetric neuronal fates in C. elegans

Electrophysiological properties and gap junction coupling of striatal astrocytes

Gap Junction Coupled Cells, Barriers and Systemic Inflammation

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