5-Aminolevulinic acid in intraoperative photodynamic therapy of bladder cancer (results of multicenter trial)

Филоненко, Е. В.; Каприн, А. Д.; Alekseev, Boris; Аполихин, О.И.; Slovokhodov, E. K.; Иванова-Радкевич, В. И.; Урлова, А. Н.

doi:10.1016/j.pdpdt.2016.09.009

Cited by 42 publications

(35 citation statements)

References 18 publications

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“…Therefore, curcumin, combined with visible light, seems a reasonable candidate to replace or complement BCG instillation therapy for patients with NMIBC. Indeed, photodynamic therapy through intravesically applied antitumor compounds has already been recommended as a highly effective concept to treat BCG refractory urothelial carcinomas [35, 36]. From a technical viewpoint, Bader et al presented evidence that transmission of 2 to 6 W white light (100 J/cm2) through a single 1.5mm diameter quartz fiber assembled into a 20 Ch transurethral irrigation catheter might provide optimum activation conditions [37].…”

Section: Discussionmentioning

confidence: 99%

The Antitumor Effect of Curcumin in Urothelial Cancer Cells Is Enhanced by Light Exposure In Vitro

Roos

Binder

Rutz

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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The natural compound curcumin exerts antitumor properties in vitro, but its clinical application is limited due to low bioavailability. Light exposure in skin and skin cancer cells has been shown to improve curcumin bioavailability; thus, the object of this investigation was to determine whether light exposure might also enhance curcumin efficacy in bladder cancer cell lines. RT112, UMUC3, and TCCSUP cells were preincubated with low curcumin concentrations (0.1-0.4 μg/ml) and then exposed to 1.65 J/cm2 visible light for 5 min. Cell growth, cell proliferation, apoptosis, cell cycle progression, and cell cycle regulating proteins along with acetylation of histone H3 and H4 were investigated. Though curcumin alone did not alter cell proliferation or apoptosis, tumor cell growth and proliferation were strongly blocked when curcumin was combined with visible light. Curcumin-light caused the bladder cancer cells to become arrested in different cell phases: G0/G1 for RT112, G2/M for TCCSUP, and G2/M- and S-phase for UMUC3. Proteins of the Cdk-cyclin axis were diminished in RT112 after application of 0.1 and 0.4 μg/ml curcumin. Cell cycling proteins were upregulated in TCCSUP and UMUC3 in the presence of 0.1 μg/ml curcumin-light but were partially downregulated with 0.4 μg/ml curcumin. 0.4 μg/ml (but not 0.1 μg/ml) curcumin-light also evoked late apoptosis in TCCSUP and UMUC3 cells. H3 and H4 acetylation was found in UMUC3 cells treated with 0.4 μg/ml curcumin alone or with 0.1 μg/ml curcumin-light, pointing to an epigenetic mechanism. Light exposure enhanced the antitumor potential of curcumin on bladder cancer cells but by different molecular action modes in the different cell lines. Further studies are necessary to evaluate whether intravesical curcumin application, combined with visible light, might become an innovative tool in combating bladder cancer.

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Section: Discussionmentioning

confidence: 99%

The Antitumor Effect of Curcumin in Urothelial Cancer Cells Is Enhanced by Light Exposure In Vitro

Roos

Binder

Rutz

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…The cancer-specific accumulation of PpIX is the key cellular mechanism used for the detection of tumours during surgeries (5-ALA-PDD) and for inducing cancer cell death by irradiating with light of specific wavelengths (5-ALA-PDT) [2][3][4][5] . We previously demonstrated that inhibiting oncogenic Ras/MEK increases PpIX accumulation in cancer cells and promotes the efficacy of 5-ALA-PDD and PDT in vitro and in vivo 14,15 .…”

Section: Discussionmentioning

confidence: 99%

MEK reduces cancer-specific PpIX accumulation through the RSK-ABCB1 and HIF-1α-FECH axes

Chelakkot

Liu

Yoshioka

et al. 2020

Sci Rep

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The efficacy of aminolevulinic acid (5-ALA)-based photodynamic diagnosis (5-ALA-PDD) and photodynamic therapy (5-ALA-PDT) is dependent on 5-ALA-induced cancer-specific accumulation of protoporphyrin IX (PpIX). We previously reported that inhibition of oncogenic Ras/MEK increases PpIX accumulation in cancer cells by reducing PpIX efflux through ATP-binding cassette sub-family B member 1 (ABCB1) and ferrochelatase (FECH)-catalysed PpIX conversion to haem. Here, we sought to identify the downstream pathways of Ras/MEK involved in the regulation of PpIX accumulation via ABCB1 and FECH. First, we demonstrated that Ras/MEK activation reduced PpIX accumulation in RasV12-transformed NIH3T3 cells and HRAS transgenic mice. Knockdown of p90 ribosomal S6 kinases (RSK) 2, 3, or 4 increased PpIX accumulation in RasV12-transformed NIH3T3 cells. Further, treatment with an RSK inhibitor reduced ABCB1 expression and increased PpIX accumulation. Moreover, HIF-1α expression was reduced when RasV12-transformed NIH3T3 cells were treated with a MEK inhibitor, demonstrating that HIF-1α is a downstream element of MEK. HIF-1α inhibition decreased FECH activity and increased PpIX accumulation. Finally, we demonstrated the involvement of RSKs and HIF-1α in the regulation of PpIX accumulation in human cancer cell lines. These results demonstrate that the RSK-ABCB1 and HIF-1α-FECH axes are the downstream pathways of Ras/MEK involved in the regulation of PpIX accumulation.

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“…administration, which is not only limiting potential skin photosensitivity, but also will limit the sensitization of the bladder musculature. The latter was demonstrated by Xiao et al preclinically and Filonenko et al clinically [15,16]. The preclinical studies showed improved survival and preservation of the bladder muscle layer using intravesical ALA or Photofrin vs intravenous photofrin.…”

Section: Introductionmentioning

confidence: 87%

Evaluation of a Ruthenium coordination complex as photosensitizer for PDT of bladder cancer: Cellular response, tissue selectivity and in vivo response

Lilge

Roufaiel²,

Lazic³

et al. 2020

Transl Biophotonics

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TLD1433, a Ru(II) polypyridyl complex, was recently introduced. It can be activated in the 520 to 540 nm range. Here we report on the use of TLD1433 for the treatment of bladder carcinomas in vitro and in an in vivo orthotopic model. The electrostatic properties of the normal urothelium vs a tumor compromised urothelium provide for a high selective uptake ratio. In vitro, a 1000 difference in the LD 50 between dark and 22.5 J cm −2 irradiation at 530 nm was achieved for two bladder cell lines (T24 and AY-27). In dogs and rats the plasma concentration for TLD1433 was below 100 ng kg −1 at 72 hours following intravenous injection at a dose of up to 3.5 mg kg −1 and below 10 ng kg −1 following intrabladder instillation for 1 hour at a concentration of 6 mg mL −1. Successful tumor necrosis close to 1.5 mm in depth was achieved.

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5-Aminolevulinic acid in intraoperative photodynamic therapy of bladder cancer (results of multicenter trial)

Cited by 42 publications

References 18 publications

The Antitumor Effect of Curcumin in Urothelial Cancer Cells Is Enhanced by Light Exposure In Vitro

The Antitumor Effect of Curcumin in Urothelial Cancer Cells Is Enhanced by Light Exposure In Vitro

MEK reduces cancer-specific PpIX accumulation through the RSK-ABCB1 and HIF-1α-FECH axes

Evaluation of a Ruthenium coordination complex as photosensitizer for PDT of bladder cancer: Cellular response, tissue selectivity and in vivo response

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