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Heim, Ute; Wang, Qing; Kurz, Thorsten; Borisjuk, Ljudmilla; Golombek, Sabine; Neubohn, Birgit; Adler, Klaus; Gahrtz, Manfred; Sauer, Norbert; Weber, Hans; Wobus, Ulrich

doi:10.1023/a:1011886908619

Cited by 21 publications

(2 citation statements)

References 37 publications

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“…While soybean SBP accelerates sucrose uptake, VfSBPL expression in yeast and transgenic potato plants did not affect in sucrose transport and carbohydrate status, respectively [ 58 ]. Interestingly, VfSBPL was expressed in seeds while GmSBP1 transcript levels were confirmed in young sink leaves [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization of sucrose binding protein as a seed-specific promoter in transgenic tobacco Nicotiana tabacum L.

et al. 2022

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Seed-specific expression using appropriate promoters is a recommended strategy for the efficiently producing valuable metabolites in transgenic plants. In the present study, we investigated the sequence of sucrose binding protein (SBP) as a seed-specific promoter to find the cis-acting elements specific to gene expression in seeds. The 1860 bp SBP sequence was analyzed using Plant Care and PLACE databases to find cis-acting elements, which resulted in a finding of 22 cis-acting elements required for seed expression. In addition, we have discovered cis- acting elements that are indirectly involved in triacylglycerol synthesis (GATABOX, DOFCOREZM, CACGTGMOTIF). The seed specificity of SBP was analyzed by generating a stable transgenic tobacco plant harboring β-glucuronidase (GUS) reporter gene under the control of the SBP promoter. Histochemical analysis of these transgenic tobacco plants indicated decreasing GUS activity in the leaves during the vegetative stage. However, the mature seeds of transgenic plants showed GUS activity. Moreover, the SBP promoter function in the seed oil content was evaluated by the expression of DGAT1. The expression analysis of DGAT1 in SBP-DGAT1 transgenic tobacco seeds using quantitative real-time PCR revealed a 7.8-fold increase in DGAT1 than in non-transgenic plants. Moreover, oil content increased up to 2.19 times more than in non-transgenic plants. And the oil content of the SBP-DGAT1 transgenic tobacco leaves did not change compared to the control plant. Therefore, we suggested that the SBP promoter could be used as a seed-specific promoter for targeted expression of desired genes in the metabolite engineering of oilseed crops.

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Section: Resultsmentioning

confidence: 99%

Characterization of sucrose binding protein as a seed-specific promoter in transgenic tobacco Nicotiana tabacum L.

et al. 2022

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“…In addition, some authors extracted from V. faba a VfSBPL ( Vicia faba sucrose binding-like protein) of 52 kDa that has a 58% homology with the 62 kDa protein contained in soy bean ( Glycine max ) [14]. …”

Section: Introductionmentioning

confidence: 99%

Vicia fabaHypersensitivity and ASA Intolerance in a Farmer: A Case Report

et al. 2011

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The IgE-mediated allergic reactions to food are caused, generally, by ingestion. However, they can be rarely induced by exposure to airborne food particles through the handling or the cooking. Vicia faba is a vegetable which belongs to Legumes or Fabaceae family, Fabales order. Allergic reactions after ingestion of legumes and cases of asthma after exposure to the cooking vapors have been reported in the literature. A paper assessed the volatile substances (insect repellents) released by V. faba. The authors demonstrated that this plant produces several chemical substances, such as small quantities of methyl salicylate. We describe a case of occupational allergy, induced by handling during picking up of fresh broad beans, in a farmer with history of adverse reaction after eating the cooked and raw vegetable.

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Vicilin and Legumin Seed Storage Proteins, Structure, Function, and Evolution of

Dunwell

2003

Biopolymers Online

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Introduction Historical Outline Classification of Globulins 11S Globulins: Legumins 7S Globulins: Vicilins Selection and Breeding Physiology and Function Deposition During Seed Development Protein Cleavage During Germination Globulins and Plant Defense Three‐dimensional Structure of Globulins Crystallization and Three‐dimensional Resolution Structure and Allergenicity of Globulins Evolution Evolution of Variation Within the Globulin Gene Family Similarity of Globulins to Other Desiccation‐associated Proteins Structural Evolution Discovery of the Cupin Superfamily Prokaryotic Ancestors of Seed Globulins Over‐expression and Modification of Globulins Expression in Bacteria Expression in Yeast Expression in Plants Other Genetic Methods for Manipulation of Globulins Recombinant Systems and Processing Quality Feeding Trials with Recombinant Globulins Production of Pharmaceutically Active Peptides in Glycinins Patents Chronological Summary of Patents Outlook and Perspectives

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Cited by 21 publications

References 37 publications

Characterization of sucrose binding protein as a seed-specific promoter in transgenic tobacco Nicotiana tabacum L.

Characterization of sucrose binding protein as a seed-specific promoter in transgenic tobacco Nicotiana tabacum L.

Vicia fabaHypersensitivity and ASA Intolerance in a Farmer: A Case Report

Vicilin and Legumin Seed Storage Proteins, Structure, Function, and Evolution of

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