3D printing of porous hydroxyapatite scaffolds intended for use in bone tissue engineering applications

Cox, Sophie C.; Thornby, John Albert; Gibbons, Gregory John; Williams, Mark A.; Mallick, Kajal K.

doi:10.1016/j.msec.2014.11.024

Cited by 402 publications

(234 citation statements)

References 33 publications

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“…These processes have been optimised over a number of decades resulting in implants that can withstand long-term cyclic loading. In recent years, the use of additive manufacturing (AM) techniques in medicine has gained much attention [17], [18], [19], [20] and [21]. Generally, AM techniques use a layer-by-layer approach to build parts from computer aided design (CAD) models.…”

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confidence: 99%

Adding functionality with additive manufacturing: Fabrication of titanium-based antibiotic eluting implants

Cox

Jamshidi

Eisenstein

et al. 2016

Materials Science and Engineering: C

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Additive manufacturing technologies have been utilised in healthcare to create patient-specific implants. This study demonstrates the potential to add new implant functionality by further exploiting the design flexibility of these technologies. Selective laser melting was used to manufacture titanium-based (Ti-6Al-4V) implants containing a reservoir. Pore channels, connecting the implant surface to the reservoir, were incorporated to facilitate antibiotic delivery. An injectable brushite, calciumphosphate cement, was formulated as a carrier vehicle for gentamicin. Incorporation of the antibiotic significantly (p=0.01) improved the compressive strength (5.8± 0.7 MPa) of the cement compared to non-antibiotic samples. The controlled release of gentamicin sulphate from the calcium phosphate cement injected into the implant reservoir was demonstrated in short term elution studies using ultraviolet visible spectroscopy. Orientation of the implant pore channels were shown, using micro-computed tomography, to impact design reproducibility and the back-pressure generated during cement injection which ultimately altered porosity. The amount of antibiotic released from all implant designs over a 6 hour period (b28% of the total amount) were found to exceed the minimum inhibitory concentrations of Staphylococcus aureus (16 μg/mL) and Staphylococcus epidermidis (1 μg/mL); two bacterial species commonly associated with periprosthetic infections. Antibacterial efficacy was confirmed against both bacterial cultures using an agar diffusion assay. Interestingly, pore channel orientation was shown to influence the directionality of inhibition zones. Promisingly, this work demonstrates the potential to additively manufacture a titanium-based antibiotic eluting implant, which is an attractive alternative to current treatment strategies of periprosthetic infections.

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mentioning

confidence: 99%

Adding functionality with additive manufacturing: Fabrication of titanium-based antibiotic eluting implants

Cox

Jamshidi

Eisenstein

et al. 2016

Materials Science and Engineering: C

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“…In the fabrication of bone and cartilage an important factor is the mechanical strength of the construct, as mechanical stability is an important function of these tissues [20,61,62]. Kang et al [63] were able to print mechanically stable constructs by co-printing cell-laden hydrogels with a biodegradable polymer.…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Current developments in 3D bioprinting for tissue engineering

Cornelissen

Faulkner-Jones

Shu

2017

Current Opinion in Biomedical Engineering

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This version is available at https://strathprints.strath.ac.uk/61660/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPTCurrent developments in 3D bioprinting for tissue engineering AbstractThe field of 3-dimensional (3D) bioprinting have enjoyed rapid development in the past few years for the applications in tissue engineering and regenerative medicine. In this review, we summarize the most updated developments in 3D bioprinting for the applications in the tissue engineering with a focus on the printable biomaterials used as bioinks. These developments include 1) novel printing regimes have been enabled by the use of fugitive inks for the creation of intricate structures e.g. vascularized tissue constructs; 2) mechanical strength of printed constructs can be enhanced by co-printing soft and hard biomaterials; 3) bioprinted in-vitro models for drug testing applications are closer to reality. We conclude that the research and application of new bioinks will remain the key highlights of the future developments in 3D bioprinting for tissue engineering.

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“…Scaffolds with design controlled structures have been obtained by means of rapid prototyping technologies including: selective laser sintering (SLS) [9], layered hydrospinning [10], laser stereolithography (SLA) [11], digital light projection (DLP) [12] or two photon polymeri zation (2PP) [13], and different materials including hydrogels [14], gelatin [7], titanium alloys [15 16], (bio)photopolymers [17] and ceramics [18]. However in vitro validation of rapid prototyped scaffolds is not commonplace, as most combinations of processes and materials do not provide adequate biomaterials and in many cases generate toxic components.…”

Section: Introductionmentioning

confidence: 99%

Composite scaffolds for osteochondral repair obtained by combination of additive manufacturing, leaching processes and hMSC-CM functionalization

Lantada

Iniesta

García–Ruiz

2016

Materials Science and Engineering: C

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A b s t r a c tArticular repair is a relevant and challenging area for the emerging fields of tissue engineering and biofabrication. The need of significant gradients of properties, for the promotion of osteochondral repair, has led to the develop ment of several families of composite biomaterials and scaffolds, using different effective approaches, although a perfect solution has not yet been found. In this study we present the design, modeling, rapid manufacturing and in vitro testing of a composite scaffold aimed at osteochondral repair. The presented composite scaffold stands out for having a functional gradient of density and stiffness in the bony phase, obtained in titanium by means of computer aided design combined with additive manufacture using selective laser sintering. The chondral phase is obtained by sugar leaching, using a PDMS matrix and sugar as porogen, and is joined to the bony phase during the polymerization of PDMS, therefore avoiding the use of supporting adhesives or additional intermediate layers. The mechanical performance of the construct is biomimetic and the stiffness values of the bony and chondral phases can be tuned to the desired applications, by means of controlled modifications of different parameters. A human mesenchymal stem cell (h MSC) conditioned medium (CM) is used for improving scaffold response. Cell culture results provide relevant information regarding the viability of the composite scaffolds used.

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3D printing of porous hydroxyapatite scaffolds intended for use in bone tissue engineering applications

Cited by 402 publications

References 33 publications

Adding functionality with additive manufacturing: Fabrication of titanium-based antibiotic eluting implants

Adding functionality with additive manufacturing: Fabrication of titanium-based antibiotic eluting implants

Current developments in 3D bioprinting for tissue engineering

Composite scaffolds for osteochondral repair obtained by combination of additive manufacturing, leaching processes and hMSC-CM functionalization

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