3D printing for human tendon-muscle gradient scaffolds engineering

Nguyen, Thi Thanh Tho; Duong, Van Thuy; Huong, Le Thi; Koo, Kyo‐in

doi:10.1109/iwis56333.2022.9920761

Cited by 2 publications

(1 citation statement)

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“…To fabricate long vasculatures with affordable systems in laboratories [38][39][40][41][42][43][44][45][46][47][48], direct extrusion techniques were developed by different research groups. The developed microfluidic devices usually induce coaxial laminar flow and simultaneously encapsulate vascular cells in hydrogels, such as decellularised extracellular matrix [49,50], type-I collagen [39], calcium alginate [51][52][53], a mixture of collagen and alginate [50,54], and customised gelatine methacryloyl (GelMA) [55].…”

Section: Introductionmentioning

confidence: 99%

Double-layered blood vessels over 3 mm in diameter extruded by the inverse-gravity technique

Duong,

Nguyen,

Phan

et al. 2023

Biofabrication

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One of the most promising techniques for treating severe peripheral artery disease is the use of cellular tissue-engineered vascular grafts (TEVGs). This study proposes an inverse-gravity (IG) extrusion technique for creating long double-layered cellular TEVGs with diameters over 3 mm. A three-layered coaxial laminar hydrogel flow in an 8 mm-diameter pipe was realized simply by changing the extrusion direction of the hydrogel from being aligned with the direction of gravity to against it. This technique produced an extruded mixture of human aortic smooth muscle cells (HASMCs) and Type-I collagen as a tubular structure with an inner diameter of 3.5 mm. After a 21-day maturation period, the maximal burst pressure, longitudinal breaking force, and circumferential breaking force of the HASMC TEVG were 416 mmHg, 0.69 N, and 0.89 N, respectively. The HASMC TEVG was endothelialized with human umbilical vein endothelial cells to form a tunica intima that simulated human vessels. Besides subcutaneous implantability on mice, the double-layered blood vessels showed a considerably lower adherence of platelets and red blood cells once exposed to heparinized mouse blood and were considered nonhemolytic. The proposed IG extrusion technique can be applied in various fields requiring multilayered materials with large diameters.

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