3D cellular spheroids as tools for understanding carboxylated quantum dot behavior in tumors

Jarockytė, Greta; Dapkutė, Dominyka; Karabanovas, Vitalijus; Daugmaudis, Justinas V.; Ivanauskas, Feliksas; Rotomskis, Ričardas

doi:10.1016/j.bbagen.2017.12.014

Cited by 28 publications

(29 citation statements)

References 38 publications

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“…32) More importantly, The expression of bile salt transporter (ASBT) in the Caco-2 cells has been reported. 33) Hence, Caco-2 cell line was chosen for in vitro studies. Preincubation with deoxycholic acid remarkably reduced the cytotoxicity of G2 against Caco-2 cells (cell viability increased from 60.59 to 72.98%) and had no obvious effects on the cytotoxicity of CPT at the same time (Fig.…”

Section: Characterization Of G2mentioning

confidence: 99%

“…Cholic acid preincubation 0.5 h significantly reduced the uptake of cholic acid-liposomes (LPs)-silybin conjugate. 33) The inhibition effects of these cholate compounds could be displayed via saturating ABST. All the results indicated that the transport pathway of G2 was changed due to conjugation with deoxycholic acid and the cellular internalization of G2 in Caco-2 cells was closely related with the uptake mediated by bile acid transporters.…”

Section: Characterization Of G2mentioning

confidence: 99%

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Transporter-Targeted Bile Acid-Camptothecin Conjugate for Improved Oral Absorption

et al. 2019

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Camptothecin (CPT), a natural alkaloid, possesses potent anticancer activity. However, its application was terminated due to its low bioavailability and high toxicity. This work evaluated the potential of deoxycholic acid-CPT conjugate (G2) to improve the oral absorption of CPT. Deoxycholic acid significantly reduced cytotoxicity and inhibited the uptake of G2, in vitro. And G2 showed sodium-dependent uptake. In addition, in vivo study in rats indicated that the oral bioavailability of G2 was 2.06-fold higher than that of CPT. The present study suggested that using bile acid as the conjugated moiety is a hopeful strategy to improve the oral bioavailability of CPT.

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Section: Characterization Of G2mentioning

confidence: 99%

Section: Characterization Of G2mentioning

confidence: 99%

Transporter-Targeted Bile Acid-Camptothecin Conjugate for Improved Oral Absorption

et al. 2019

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“…The results from the proposed model, in combination with the experimental results, suggested that particle size, particle binding, and porosity of biological tissue are the key parameters that need to be considered when designing NP drug carriers for cancer treatment. Our previous experimental study and modeling results also demonstrated that penetration of carboxylated nanoparticles was strongly limited and dependent on size and porosity of cellular spheroids [14].…”

Section: Introductionmentioning

confidence: 77%

Modeling the uptake of fluorescent molecules into 3D cellular spheroids

Astrauskas

Ivanauskas

Jarockytė³

et al. 2019

NAMC

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Three mathematical models were developed to analyze the dynamics of fluorescent dyes penetration into 3D cellular spheroids. Two fluorescent dyes were chosen to verify mathematical models: rhodamine 6G (R6G) as a small molecule, which can freely penetrate through the cells, and wheat germ agglutinin (WGA) conjugated with Alexa488 fluorescent label, which reacts with the cells plasma membrane, and its cellular penetration is significantly lower. Dye penetration and binding to cells were modeled with nonlinear diffusion–reaction equations. System of differential equations was solved using numerical methods, and good correspondence with physical experiment was shown. Diffusion coefficients in extracellular matrix were determined for both fluorescent dyes, and the influence of reactions parameters to WGA penetration was analyzed. Dynamics of dyes accumulation into cell spheroids were also determined.

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“…Tri‐dimensional cell culture platforms have been a subject of interest since they demonstrate significant differences in cell differentiation and behaviour compared to conventional 2D systems . Considering the advantages of 3D cell culture models particularly in cancer research, we compared our cytotoxicity results with 2D and 3D cell models.…”

Section: Resultsmentioning

confidence: 99%

Assessing the cytotoxic potential of glycoalkaloidic extract in nanoparticles against bladder cancer cells

Miranda

Marcato

Carvalho

et al. 2019

Journal of Pharmacy and Pharmacology

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Objective This study proposed to use the nanotechnology to deliver glycoalkaloidic extract (AE) to bladder cancer cells, evaluating their activity in 2D and 3D models and the biological mechanism of cell death. Methods NPs were prepared by nanoprecipitation method using polylactic acid (PLA) and characterized considering their size, charge, particle concentration and stability. The cytotoxicity was evaluated in 2D and 3D model, and the apoptosis and cell cycle were investigated using flow cytometry. Key findings NPs loading AE (NP‐AE) had diameter around 125 ± 6 nm (PdI <0.1) and negative charge. The encapsulation efficiency of SM and SS was higher than 85% for both compounds. The obtained formulation showed a significant in‐vitro cytotoxic effect against RT4 cells in a dose‐dependent manner with IC50 two fold lower than the free AE. The cytotoxic effect of NP‐AE was mediated by apoptosis and cell cycle arrested in the S phase. RT4 cells cultured under 3D conditions exhibited a higher resistance to the treatments (IC50 ~ three fold higher than in 2D cell culture). Conclusion The NP‐AE might be a promising nanocarrier to load and deliver glycoalkaloids against bladder cancer.

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3D cellular spheroids as tools for understanding carboxylated quantum dot behavior in tumors

Cited by 28 publications

References 38 publications

Transporter-Targeted Bile Acid-Camptothecin Conjugate for Improved Oral Absorption

Transporter-Targeted Bile Acid-Camptothecin Conjugate for Improved Oral Absorption

Modeling the uptake of fluorescent molecules into 3D cellular spheroids

Assessing the cytotoxic potential of glycoalkaloidic extract in nanoparticles against bladder cancer cells

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