Untitled

Kumria, R.; Waie, B.; Rajam, Manchikatla Venkat

doi:10.1023/a:1011645315304

Cited by 49 publications

(6 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A half-strength of MS medium and a lesser concentration of phytagel was maintained to facilitate better proliferation of roots (Ramesh et al 2009). The Acetosyringone concentration, induction of Agrobacterium, infection and co-cultivation time were optimised for in-vitro transformation, as these are crucial parameters determining its success (Declerck et al 2002;Kumria et al 2001;Turk et al 1991). Co-cultivation was done in dark to prevent accumulation of phenolics and degradation of lightsensitive auxins (Arezki et al 2001).…”

Section: Resultsmentioning

confidence: 99%

Optimisation of a rapid and efficient transformation protocol for fungal blast-susceptible indica rice cultivars HR-12 and CO-39

Sarkar¹,

Srinivasan

Roy-Barman³

2021

Plant Biotechnology

View full text Add to dashboard Cite

Rice is an important staple crop and fungal blast disease destroys about 10-30% of its global produce, annually. Although genetic manipulation has largely been employed in crop-improvement programmes and agricultural biotechnology, the ease of transformation of several recalcitrant indica cultivars continues to be a challenge. HR-12 and CO-39 are two indica cultivars that are commonly used in breeding programmes, but are susceptible to biotic threats like fungal blast and sheath blight disease. Here in this study, we have optimised a rapid and reproducible transformation protocol for the said cultivars, having compared both the tissue-culture and in-planta methods of transformation. Murashige & Skoog basal media supplemented with maltose and 2.5 mg l −1 2,4-D induced efficient callogenesis in HR-12, while maltose with 3 mg l −1 2,4-D gave optimum results in case of CO-39. The media containing 0.5 mg l −1 NAA, 3 mg l −1 BAP, and 1 mg l −1 kinetin yielded a maximum regeneration efficiency of 62% and 65% in HR-12 and CO-39, respectively. The studies with Agrobacterium tumefaciens, LBA4404 strain harbouring pCAMBIA1303 suggested that although these cultivars demonstrated successful gene-transfer, they failed to regenerate efficiently, post-transformation. Alternatively, our modified in-planta piercing and vacuum infiltration-based protocol resulted in 33-35% transformation efficiency in less than half the time required for tissue-culture based transformation method. As per our knowledge, it is among the highest obtained from existing piercing-based direct transformation protocols in rice, and can also be implemented in genetically manipulating other recalcitrant varieties of rice.

show abstract

Section: Resultsmentioning

confidence: 99%

Optimisation of a rapid and efficient transformation protocol for fungal blast-susceptible indica rice cultivars HR-12 and CO-39

Sarkar¹,

Srinivasan

Roy-Barman³

2021

Plant Biotechnology

View full text Add to dashboard Cite

show abstract

“…Sponges are another insulin delivery form, particularly in the buccal route. [70,136] Chitosan sponges plus ethylcellulose as an impermeable layer protect insulin against leakage. The swelling or dissolving properties of the chitosan sponge resulted in insulin diffusion in the matrix, which is a key factor influencing the releasing profile.…”

Section: Macro-formulationsmentioning

confidence: 99%

A Review on Bioengineering Approaches to Insulin Delivery: A Pharmaceutical and Engineering Perspective

Taraghdari

Imani

Mohabatpour

2019

Macromolecular Bioscience

View full text Add to dashboard Cite

Diabetes mellitus (DM) is the most prevalent non‐contagious disease, which has affected a large number of people all over the world. Among all treatments known to have a positive influence in the control of DM, insulin therapy is the most common and effective one. Nowadays, various methods of insulin delivery are under investigation, which are able to reach a plausible bioavailability with ignorable side effects instead of insulin injection. This article presents a comprehensive review of the insulin therapy approach with a focus on modified methods in insulin delivery strategies and current advances in engineered insulin delivery systems.

show abstract

“…We observed significant improvements in transformation efficiency when a high concentration of maltose was used instead of sucrose. Maltose has been given much importance as an alternative carbon source in a medium for better regeneration of some plants (Kumria et al 2001). It has also been used in the biolistic transformation of Lilium (Cohen et al 2004).…”

Section: Macronutrient-free Medium Recommended For Lilium Transformationmentioning

confidence: 99%

Efficient Agrobacterium-mediated transformation of Lilium Oriental ‘Sorbonne’ with genes encoding anthocyanin regulators

Wei¹,

Cui²,

Yuan³

et al. 2017

Can. J. Plant Sci.

View full text Add to dashboard Cite

Plant anthocyanin biosynthesis is regulated by a combination of transcription factors. Here, exogenous anthocyanin regulator genes Delila/Rosea1 (Del/Ros1) or Bperu/Colored aleurone1 (B1/C1) were introduced to Lilium 'Sorbonne' using an efficient and optimized Agrobacterium tumefaciens (Smith & Towns.) Conn-mediated transformation system. During the B1/C1 transformation, eight types of modified Murashige and Skoog medium for bacterial inoculation and co-cultivation were evaluated. Significant enhancement in putative transformation efficiency was observed upon the removal of KH 2 PO 4 , NH 4 NO 3 , and KNO 3 or with the removal of all the macroelements. Using the optimal medium, 20 s sonication positively affected the transformation while no significant improvement was observed following heat shock treatments. Replacing sucrose in the co-cultivation medium with high concentrations of maltose or glucose also promoted the transformation. Higher transformation efficiencies were observed when using this optimized protocol for the Del/Ros1 transformation. The Del/Ros1 and B1/C1 transformed plantlets appeared light purple during the preliminary regeneration stage, with some deeper purple tissues also observed in Del/Ros1 plantlets. After the transformation was confirmed by polymerase chain reaction and Southern blot, the colour unexpectedly faded to normal green when the transgenic plantlets were rooted, but significant elevations in anthocyanin levels remained in leaves and scales compared with the nontransgenic plantlets. The introduction of these anthocyanin regulator genes may be useful for the modification of Lilium flower colour.

show abstract

Untitled

Cited by 49 publications

References 19 publications

Optimisation of a rapid and efficient transformation protocol for fungal blast-susceptible indica rice cultivars HR-12 and CO-39

Optimisation of a rapid and efficient transformation protocol for fungal blast-susceptible indica rice cultivars HR-12 and CO-39

A Review on Bioengineering Approaches to Insulin Delivery: A Pharmaceutical and Engineering Perspective

Efficient Agrobacterium-mediated transformation of Lilium Oriental ‘Sorbonne’ with genes encoding anthocyanin regulators

Contact Info

Product

Resources

About