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Sailaja, P.; Podile, Appa Rao; Reddanna, Pallu

doi:10.1023/a:1008638608639

Cited by 42 publications

(3 citation statements)

References 28 publications

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“…In many pathosystems, two defense pathways are generally associated with the enhanced protection level conferred by ISR: the phenylpropanoid pathway and the oxylipin pathway. Bacillus cereus B101R and B. subtilis AF 1 induce lipoxygenase (LOX) activity in tomato [11] and groundnut [12] respectively. This enzyme is a dioxygenase that transforms poly-unsaturated fatty acids into hydroperoxides.…”

Section: Introductionmentioning

confidence: 99%

The elicitation of a systemic resistance by Pseudomonas putidaBTP1 in tomato involves the stimulation of two lipoxygenase isoforms

et al. 2011

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BackgroundSome non-pathogenic rhizobacteria called Plant Growth Promoting Rhizobacteria (PGPR) possess the capacity to induce in plant defense mechanisms effective against pathogens. Precedent studies showed the ability of Pseudomonas putida BTP1 to induce PGPR-mediated resistance, termed ISR (Induced Systemic Resistance), in different plant species. Despite extensive works, molecular defense mechanisms involved in ISR are less well understood that in the case of pathogen induced systemic acquired resistance.ResultsWe analyzed the activities of phenylalanine ammonia-lyase (PAL) and lipoxygenase (LOX), key enzymes of the phenylpropanoid and oxylipin pathways respectively, in tomato treated or not with P. putida BTP1. The bacterial treatment did not stimulate PAL activity and linoleate-consuming LOX activities. Linolenate-consuming LOX activity, on the contrary, was significantly stimulated in P. putida BTP1-inoculated plants before and two days after infection by B. cinerea. This stimulation is due to the increase of transcription level of two isoforms of LOX: TomLoxD and TomLoxF, a newly identified LOX gene. We showed that recombinant TomLOXF preferentially consumes linolenic acid and produces 13-derivative of fatty acids. After challenging with B. cinerea, the increase of transcription of these two LOX genes and higher linolenic acid-consuming LOX activity were associated with a more rapid accumulation of free 13-hydroperoxy-octadecatrienoic and 13-hydroxy-octadecatrienoic acids, two antifungal oxylipins, in bacterized plants.ConclusionIn addition to the discovery of a new LOX gene in tomato, this work is the first to show differential induction of LOX isozymes and a more rapid accumulation of 13-hydroperoxy-octadecatrienoic and 13-hydroxy-octadecatrienoic acids in rhizobacteria mediated-induced systemic resistance.

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Section: Introductionmentioning

confidence: 99%

The elicitation of a systemic resistance by Pseudomonas putidaBTP1 in tomato involves the stimulation of two lipoxygenase isoforms

et al. 2011

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“…Metabolic products of polyunsaturated fatty acids have been variously implicated in control of microbial pathogens. 13(S)-HpOTrE, a monohydroperoxy polyunsaturated fatty acid, which was up-regulated in T2 and mainly found in the placenta tissue of the pepper, may take part in a lipid-based signaling system initiated by insect and pathogen attack [66]. The complex metabolic reactions and their regulation in pepper plants are not carried out alone.…”

Section: Ammonium: Nitrate Ratio Affects Metabolites Of Peppermentioning

confidence: 99%

Nitrogen Source Affects the Composition of Metabolites in Pepper (Capsicum annuum L.) and Regulates the Synthesis of Capsaicinoids through the GOGAT–GS Pathway

Zhang

Xie

et al. 2020

Foods

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Phytochemical analyses of pepper fruit metabolites have been reported; however, much less is known about the influence of different forms of nitrogen (N), which is critical for plant growth and fruit quality formation. The “Longjiao No. 5” variety (Capsicum annuum L.) grown in Northwestern China was profiled using liquid chromatography–mass spectrometry (LC–MS) coupled with multivariate data analysis to explore the composition of different metabolites in pericarp and placenta, and to investigate the effect of three ammonium (NH4+) to-nitrate (NO3−) ratios (0:100, 25:75, and 50:50). A total of 215 metabolites were obtained by qualitative analysis, where 31 metabolites were the major differential metabolite components of pepper fruits between placenta and pericarp, and 25 among N treatments. The addition of ammonium up-regulated carbohydrates, such as Î±-lactose and sucrose, as well as phenylalanine lyase (PAL) of placenta tissue. The supply of 25% NH4+–N and 75% NO3−–N exhibited a relatively higher levels of ascorbic acid in pericarp and amino acids, capsaicin, and dihydrocapsaicin in placenta, and led to higher fruit weight among the ammonium-to-nitrate ratios. The expression and activities of glutamic acid synthetase (GOGAT) and glutamine synthetase (GS) that are involved in ammonium assimilation were affected by adjusting the ammonium–N proportion, and they were significantly positively correlated with capsaicin, dihydrocapsaicin contents, capsaicinoid synthetase (CS), as well as the relative expression levels of genes related to capsaicinoid biosynthesis, such as acyltransferase 3 (AT3) and acyl-ACP thioesterase (FatA).

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“…Many Bacillus species such as B. subtilis , B. cereus , B. amyloliquefaciens , B. licheniformis , B. pumilus , B. brevis , B. coagulans and B. laterosporus , etc., have been isolated and identified in post-fermented tea [ 10 , 19 ]. B. subtilis AF1 can inhibit the growth of A. niger by destroying the cell wall of A. niger [ 20 ]. B. licheniformis BL350–2 isolated from raspberry and strawberry jams inhibited the growth of mycotoxigenic species such as A. carbonarius , A. ochraceus , Penicillium verrucosum , etc.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effect of Polypeptides Produced by Brevibacillus brevis on Ochratoxigenic Fungi in the Process of Pile-Fermentation of Post-Fermented Tea

Zhao

Zhang

Lou

et al. 2022

Foods

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Contamination by ochratoxigenic fungi and its prevention during the pile-fermentation of post-fermented tea have always been a concern. The present study aimed to elucidate the anti-fungal effect and mechanism of polypeptides produced by B. brevis DTM05 (isolated from post-fermented tea) on ochratoxigenic fungi, and to to evaluate their use in the pile-fermentation process of post-fermented tea. The results showed that polypeptides (produced by B. brevis DTM05) with a strong antifungal effect against A. carbonarius H9 mainly had a molecular weight between 3 and 5 kDa. The Fourier-transform infrared spectra of this polypeptide extract showed that it was a mixture consisting mainly of polypeptides and small amounts of lipids and other carbohydrates. The polypeptide extracts significantly inhibited the growth of A. carbonarius H9, and its minimum inhibitory concentration (MIC) was 1.6 mg/L, which significantly reduced the survival rate of spores. The polypeptides also effectively controlled the occurrence and ochratoxin A (OTA) production of A. carbonarius H9 on the tea matrix. The lowest concentration of polypeptides that significantly inhibited the growth of A. carbonarius H9 on the tea matrix was 3.2 mg/L. The enhancement of the fluorescence staining signal in the mycelium and conidiospore showed that the polypeptides with a concentration of more than 1.6 mg/L increased the permeability of the mycelium membrane and conidial membrane of A. carbonarius H9. The significant increase in the extracellular conductivity of mycelia suggested the outward leakage of intracellular active substances, and also further indicated an increase in cell membrane permeability. Polypeptides with a concentration of 6.4 mg/L significantly down-regulated the expression level of the polyketide synthase gene related to OTA production (acpks) in A. carbonarius H9, which may be the fundamental reason why polypeptides affect OTA production. In conclusion, reasonable use of the polypeptides produced by B. brevis can destroy the structural integrity of the cell membrane, make the intracellular active substances leak outward, accelerate the death of fungal cells and down-regulate the expression level of the polyketide synthase gene in A. carbonarius; thus, they can effectively control the contamination of ochratoxigenic fungi and OTA production during the pile-fermentation of the post-fermented tea.

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Cited by 42 publications

References 28 publications

The elicitation of a systemic resistance by Pseudomonas putidaBTP1 in tomato involves the stimulation of two lipoxygenase isoforms

The elicitation of a systemic resistance by Pseudomonas putidaBTP1 in tomato involves the stimulation of two lipoxygenase isoforms

Nitrogen Source Affects the Composition of Metabolites in Pepper (Capsicum annuum L.) and Regulates the Synthesis of Capsaicinoids through the GOGAT–GS Pathway

Inhibitory Effect of Polypeptides Produced by Brevibacillus brevis on Ochratoxigenic Fungi in the Process of Pile-Fermentation of Post-Fermented Tea

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