[34] Membrane receptor for phosphomannosyl residues

Sahagian, G. Gary; Distler, Jack J.; Jourdian, George W.

doi:10.1016/0076-6879(82)83036-x

Cited by 68 publications

(28 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yeast phosphomannan was very kindly provided by Dr. M. E. Slodki (U. S. Department of Agriculture, Peoria, IL). Phosphomannan affinity resin was prepared by coupling cyanogen bromide-activated Sepharose CL-6B (Pharmacia) to phosphomannan core as described previously (27). Recombinant human IGF-IIs with and without a 21-amino acid E-peptide extension (rhIGF-IIE 88 and rhIGF-II, respectively) were as described (28,29).…”

Section: Methodsmentioning

confidence: 99%

Biophysical and Biological Properties of Naturally Occurring High Molecular Weight Insulin-like Growth Factor II Variants

Valenzano¹,

Heath-Monnig

Tollefsen

et al. 1997

Journal of Biological Chemistry

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Biophysical and Biological Properties of Naturally Occurring High Molecular Weight Insulin-like Growth Factor II Variants

Valenzano¹,

Heath-Monnig

Tollefsen

et al. 1997

Journal of Biological Chemistry

View full text Add to dashboard Cite

“…Mouse monoclonal antibodies to AP-1, EEA1, GM130, syntaxin 6, and the EGF receptor (EGFR) were purchased from Transduction Laboratories (Lexington, KY). Rabbit polyclonal antibodies to rat MPR300 were raised against either a fusion protein encoding glutathion S -transferase coupled to a portion of the cytoplasmic tail (amino acids 2,313 to 2,409) of rat MPR300 (22) or purified receptor from bovine liver by penta phosphomannan affinity chromatography as described previously (23). Mouse monoclonal antibody to the bovine MPR300 (MA1-066) was obtained from Affinity BioReagents (Golden, CO).…”

Section: Antibodiesmentioning

confidence: 99%

Distribution and trafficking of MPR300 is normal in cells with cholesterol accumulated in late endocytic compartments

Umeda

Fujita

Kuronita

et al. 2003

Journal of Lipid Research

View full text Add to dashboard Cite

It has been reported that an accumulation of cholesterol within late endosomes/lysosomes in Niemann-Pick type C (NPC) fibroblasts and U18666A-treated cells causes impairment of retrograde trafficking of the cation-independent mannose 6-phosphate/IGF-II receptor (MPR300) from late endosomes to the trans -Golgi network (TGN). In apparent conflict with these results, here we show that as in normal fibroblasts, MPR300 localizes exclusively to the TGN in NPC fibroblasts as well as in normal fibroblasts treated with U18666A. This localization can explain why several lysosomal properties and functions, such as intracellular lysosomal enzyme activity and localization, the biosynthesis of cathepsin D, and protein degradation, are all normal in NPC fibroblasts. These results, therefore, suggest that the accumulation of cholesterol in late endosomes/lysosomes does not affect the retrieval of MPR300 from endosomes to the TGN. Furthermore, treatment of normal and NPC fibroblasts with chloroquine, which inhibits membrane traffic from early endosomes to the TGN, resulted in a redistribution of MPR300 to EEA1 and internalized transferrin-positive, but LAMP-2-negative, early-recycling endosomes. We propose that in normal and NPC fibroblasts, MPR300 is exclusively targeted from the TGN to early endosomes, from where it rapidly recycles back to the TGN without being delivered to late endosomes. This notion provides important insights into the definition of late endosomes, as well as the biogenesis of lysosomes. Two distinct and related mannose 6-phosphate receptors (MPRs), the cation-dependent mannose 6-phosphate receptor (MPR46) and the cation-independent mannose 6-phosphate/IGF-II receptor (MPR300), are responsible for sorting soluble lysosomal enzymes from the secretory pathway. They have a largely overlapping but apparently distinct affinity for lysosomal enzymes (1, 2). Newly synthesized lysosomal enzymes acquire mannose 6-phosphate residues in the Golgi complex and are recognized in the trans -Golgi network (TGN) by MPRs [reviewed in refs. (3,4)]. The ligand-receptor complex is subsequently packed into clathrin-coated vesicles and targeted to endosomal compartments. The acidic environment of endosomes causes the release of the enzymes from MRPs, after which the lysosomal enzymes are transferred to lysosomes while the MPRs are retrieved to the TGN for further rounds of sorting. Therefore, the MPRs play a key role in the biogenesis and function of lysosomes.Intracellular trafficking of MPRs is mediated by signals present in their cytoplasmic tails, which contain two signals, tyrosine-and dileucine-based sorting signals, as well as by posttranslational modifications, such as the phosphorylation of serine residues and the palmitoylation of cysteine residues (3, 4). Notably, an acidic cluster-dileucine motif that consists of a cluster of acidic amino acid residues followed by dileucine residues is important to the sorting of MPRs from the TGN to endosomes. Recent progress in the study of MPR trafficking from the TGN to e...

show abstract

“…Originally isolated from bovine hepatocyte plasma membranes (5), PMR was subsequently prepared by affinity chromatography from bovine liver (16), Swarm rat chondrosarcoma (6), monkey brain (17), and rat spleen (2). Each preparation, with the exception of monkey brain, when subjected to one-dimension (iD) PAGE under reducing conditions exhibited an apparent Mr of 215,000.…”

mentioning

confidence: 99%

“…PMR was isolated by the method of Sahagian et al (16) from the livers of exsanguinated Syrian hamsters and was stored at 4°C in the presence of 5 mM Man-6-P until used.…”

mentioning

confidence: 99%

Properties of the Syrian hamster phosphomannosyl receptor: an aggregate of low molecular weight proteins.

Maler¹,

Rosenblum²,

Jourdian³

1985

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

[34] Membrane receptor for phosphomannosyl residues

Cited by 68 publications

References 3 publications

Biophysical and Biological Properties of Naturally Occurring High Molecular Weight Insulin-like Growth Factor II Variants

Biophysical and Biological Properties of Naturally Occurring High Molecular Weight Insulin-like Growth Factor II Variants

Distribution and trafficking of MPR300 is normal in cells with cholesterol accumulated in late endocytic compartments

Properties of the Syrian hamster phosphomannosyl receptor: an aggregate of low molecular weight proteins.

Contact Info

Product

Resources

About