Morpho‐Functional Characteristics of Bone Marrow Multipotent Mesenchymal Stromal Cells after Activation or Inhibition of Epidermal Growth Factor and Toll‐Like Receptors or Treatment with DNA Intercalator Cisplatin

Abstract

This study is aimed to reveal morphological and functional changes in multipotent mesenchymal stromal cells (MSCs) isolated from the rat bone marrow after: (i) activation of Toll-like receptors (TLRs) with teichoic acid (TA), (ii) impact on epidermal growth factor (EGF) receptors with activator EGF or inhibitor Herceptin, and (iii) treatment with DNA intercalator Cisplatin. According to our results, TA and EGF cause an increase in the synthesis of glycosaminoglycans, c-Myc content, and protein in the MSC cytoplasm. It was observed that the cell population in G0 phase decreased and the cell population in G1 phase increased, when compared with control. At the same time, the cell population with a higher nuclear-cytoplasmic ratio (NCR) in S and G2 phases also increased. This indicates the manifestation of the MSC mesenchymal phenotype, exhibiting indirect metabolic signs of the regenerative potential increase. In other experiments, Herceptin was shown to suppress only the stemness signs of MSCs, while Cisplatin seriously affected cell viability in general, reducing synthetic and proliferative activities and causing cell morphology disturbances.

HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution

“…Studies on cervical epithelial cells based on Fourier Transform Infrared Spectroscopy (FTIR) suggested that for dysplastic and neoplastic changes, a characteristic decrease in the intensity of glycogen-specific bands was observed [37]. Glycogen is one of the compounds accumulated intracellularly in animal cells in the form of stores with a diameter of 10 to 40 nm [38]. Already in the 1930s, Schiller determined that cervical cancer cells, unlike healthy cells, lack glycogen [39].…”

Many shades of sorting

“…It is impossible for me to ignore the actual political situation and the threat, cruelty and pain that our colleagues and friends, scientific authors (1,2), readership and their relatives and friends from Ukraine are facing now. I'm expressing my deep compassion to all of you, and I think I am writing here in the name of the majority of the worldwide cytometric and academic community.…”