Sperm-mediated DNA lesions alter metabolite levels in spent embryo culture medium

Souza, F. de; Asampille, Gitanjali; Uppangala, Shubhashree; Kalthur, Guruprasad; Atreya, Hanudatta S.; Adiga, Satish Kumar

doi:10.1071/rd18136

Cited by 5 publications

(5 citation statements)

References 34 publications

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“…D'Souza et al [93] used mouse embryo SCM to predict the implantation ability of the embryos which showed the increased requirement of pyruvate and lactate accompanied by a significant reduction of pyruvate/alanine ratio in the SCM. Further, sperm-induced genetic lesions in the embryos are associated with a significant increase in the uptake of pyruvate and reduced pyruvate/alanine ratio in SCM [113]. Data from animal studies provide only fundamental knowledge but cannot be directly extrapolated to human ART due to species-specific differences in the metabolic requirements, chromatin architecture and developmental timelines.…”

Section: Composition Of the Different Embryo Culture Mediamentioning

confidence: 99%

The utility of nuclear magnetic resonance spectroscopy in assisted reproduction

et al. 2020

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Infertility affects approximately 15–20% of individuals of reproductive age worldwide. Over the last 40 years, assisted reproductive technology (ART) has helped millions of childless couples. However, ART is limited by a low success rate and risk of multiple gestations. Devising methods for selecting the best gamete or embryo that increases the ART success rate and prevention of multiple gestation has become one of the key goals in ART today. Special emphasis has been placed on the development of non-invasive approaches, which do not require perturbing the embryonic cells, as the current morphology-based embryo selection approach has shortcomings in predicting the implantation potential of embryos. An observed association between embryo metabolism and viability has prompted researchers to develop metabolomics-based biomarkers. Nuclear magnetic resonance (NMR) spectroscopy provides a non-invasive approach for the metabolic profiling of tissues, gametes and embryos, with the key advantage of having a minimal sample preparation procedure. Using NMR spectroscopy, biologically important molecules can be identified and quantified in intact cells, extracts or secretomes. This, in turn, helps to map out the active metabolic pathways in a system. The present review covers the contribution of NMR spectroscopy in assisted reproduction at various stages of the process.

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Section: Composition Of the Different Embryo Culture Mediamentioning

confidence: 99%

The utility of nuclear magnetic resonance spectroscopy in assisted reproduction

et al. 2020

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“…Paternal genetic alterations may affect embryo viability and reproductive outcomes. It has been shown that paternally transmitted DNA lesions influence the metabolism in preimplantation embryos [31,56]. Low glutamine level was found on day 3 SCM in male factor patients [31].…”

Section: Fig 2)mentioning

confidence: 99%

“…Since ICSI completely bypasses natural sperm selection, we investigated the differences in the level of metabolites in relation to sperm DNA fragmentation and sperm head defects. Embryos derived from DNA fragmented spermatozoa progressed normally until the blastocyst stage, showed reduced pyruvate-to-alanine ratio in SCM [56]. Hence, we tested the spent medium from the optimal quality blastocyst on day 5.…”

Section: Fig 2)mentioning

confidence: 99%

ICSI in non-male factor infertility patients does not alter metabolomic signature in sibling embryos as evidenced by sensitivity enhanced nuclear magnetic resonance (NMR) spectroscopy

et al. 2022

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Intracytoplasmic sperm injection (ICSI) was developed to overcome male factor infertility, however, there recently has been an increasing trend in ICSI usage irrespective of the etiology, demonstrating an overuse of this insemination technique. There is a limited knowledge on the behaviour of ICSI derived embryos in non-male factor infertility patients. Metabolomic assessment of preimplantation embryos in conjunction with morphological evaluation can provide better understanding of embryonic behaviour. Hence, this study was undertaken to explore if there are any metabolomic differences between IVF and ICSI derived sibling day-5 blastocysts from non-male factor infertility patients. This prospective study included nineteen couples with non-male factor infertility undergoing Assisted Reproductive Technology. The sibling oocytes retrieved from each patient were randomly assigned to two groups and inseminated either by IVF or ICSI. Spent culture media (SCM) in which embryos were cultured up to day 5 were collected and investigated using sensitivity enhanced NMR based metabolite profiling utilizing high resolution (800 MHz) NMR equipped with cryogenically cooled micro-coil (1.7 mm) probe. The metabolomic signature between IVF and ICSI derived sibling blastocysts was assessed. A significant reduction in the concentrations of pyruvate, citrate, glucose and lysine were observed in both IVF and ICSI sibling embryos compared to medium control (P< 0.05–0.001). Further, histidine and valine level was found lower in ICSI embryos compared to medium control (P<0.05) during 96 hours of in vitro culture. Notably, between IVF and ICSI SCM, no significant difference in the concentration of the metabolites was found. Our results suggest that ICSI in non-male factor does not alter the SCM metabolomic signature during 96 hours of embryonic development.

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“…Therefore, most of the studies in the literature have correlated the embryonic response to the sperm OS with reference to the sperm DNA damage. The response to the entry of spermatozoa carrying DNA damage to the ooplasm during fertilization can have various consequences [81,82,[186][187][188][189]. Depending on the extent of sperm DNA damage, the oocyte can repair the damage in spermatozoa and lead to a healthy embryo; it can successfully fertilize with the development of the defective embryo, or it may lead to fertilization failure or embryo arrest [190].…”

Section: Effects Of Sperm Os On Embryo Developmentmentioning

confidence: 99%

Sperm Oxidative Stress during In Vitro Manipulation and Its Effects on Sperm Function and Embryo Development

et al. 2021

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Reactive oxygen species (ROS) generated at low levels during mitochondrial respiration have key roles in several signaling pathways. Oxidative stress (OS) arises when the generation of ROS exceeds the cell’s antioxidant scavenging ability and leads to cell damage. Physiological ROS production in spermatozoa regulates essential functional characteristics such as motility, capacitation, acrosome reaction, hyperactivation, and sperm-oocyte fusion. OS can have detrimental effects on sperm function through lipid peroxidation, protein damage, and DNA strand breakage, which can eventually affect the fertility of an individual. Substantial evidence in the literature indicates that spermatozoa experiencing OS during in vitro manipulation procedures in human- and animal-assisted reproduction are increasingly associated with iatrogenic ROS production and eventual impairment of sperm function. Although a direct association between sperm OS and human assisted reproductive techniques (ART) outcomes after in vitro fertilization (IVF) and/or intracytoplasmic sperm injection (ICSI) is still a matter of debate, studies in animal models provide enough evidence on the adverse effects of sperm OS in vitro and defective fertilization and embryo development. This review summarized the literature on sperm OS in vitro, its effects on functional ability and embryo development, and the approaches that have been proposed to reduce iatrogenic sperm damage and altered embryonic development.

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Sperm-mediated DNA lesions alter metabolite levels in spent embryo culture medium

Cited by 5 publications

References 34 publications

The utility of nuclear magnetic resonance spectroscopy in assisted reproduction

The utility of nuclear magnetic resonance spectroscopy in assisted reproduction

ICSI in non-male factor infertility patients does not alter metabolomic signature in sibling embryos as evidenced by sensitivity enhanced nuclear magnetic resonance (NMR) spectroscopy

Sperm Oxidative Stress during In Vitro Manipulation and Its Effects on Sperm Function and Embryo Development

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