2018
DOI: 10.1073/pnas.1810229115
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Abstract: RNA interference (RNAi) is the major antiviral defense mechanism of plants and invertebrates, rendering the capacity to evade it a defining factor in shaping the viral landscape. Here we sought to determine whether different virus replication strategies provided any inherent capacity to evade RNAi in the absence of an antagonist. Through the exploitation of host microRNAs, we recreated an RNAi-like environment in vertebrates and directly compared the capacity of positive- and negative-stranded RNA viruses to c… Show more

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Cited by 17 publications
(9 citation statements)
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“…If a cell in the host becomes infected with both strains, then recombination can occur. recombination in RNA viruses has been demonstrated to be an intrinsic defense against host antiviral RNA interference (Aguado et al, 2018). For a review of recombination in RNA viruses, see Worobey andHolmes, 1999 or Simon-Loriere andHolmes, 2011. Because of their high prevalence in juvenile animals and sufficient genetic similarity (in conserved regions), many examples of astrovirus recombination have been characterized.…”
Section: Recombinationmentioning
confidence: 99%
“…If a cell in the host becomes infected with both strains, then recombination can occur. recombination in RNA viruses has been demonstrated to be an intrinsic defense against host antiviral RNA interference (Aguado et al, 2018). For a review of recombination in RNA viruses, see Worobey andHolmes, 1999 or Simon-Loriere andHolmes, 2011. Because of their high prevalence in juvenile animals and sufficient genetic similarity (in conserved regions), many examples of astrovirus recombination have been characterized.…”
Section: Recombinationmentioning
confidence: 99%
“…To this end, we tested the robustness of the viral ribonucleoprotein (RNP) complex to NP perturbations, and investigated their effects in the production and recognition of PAMPs. In order to generate an imbalance in NP production, we engineered recombinant strains of IAV and Sendai virus (SeV) in which the NP transcript would be the target of host-derived miRNAs previously shown to exert potent silencing activity in a broad range of cells (Figure S1A-C) (Aguado et al, 2018).…”
Section: Characterizing Negative-sense Rna Virus Replication Under LImentioning
confidence: 99%
“…Infectious titers of influenza A viruses were determined by plaque assays in MDCK or MDCK-NS1 cells. Influenza A/Puerto Rico/8/34 (H1N1) virus and Sendai virus containing a miRNA-silencing cassette targeting viral NP transcripts (IAV-NP-T and SeV-NP-T, respectively) as well as their respective control viruses (IAV-NP-C and SeV-NP-C) were rescued as previously described (Aguado et al, 2018). IAV-NP-T, IAV-NP-C, and SeV-NP-C were grown in 10-day old specific pathogenfree (SPF) chicken eggs (Charles River Laboratories).…”
Section: Virusesmentioning
confidence: 99%
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“…However, as this difference in biology is almost completely defined by complementarity, if one introduces a perfect binding site for a given miRNA into a transcript, it will undergo potent silencing more akin to the normal activity of antiviral RNAi 19 . This exploitation of miRNA biology therefore enables one to generate viruses or virus-based vectors which can be ubiquitously targeted or function in a species-, tissue-, cell-, or even signaling-specifc manner based on endogenous miRNA expression 2026 .…”
Section: Introductionmentioning
confidence: 99%