Coagulation Factor Activities Changes Over 5 Days in Thawed Fresh Frozen Plasma Stored at Different Initial Storage Temperatures

Noordin, Siti Salmah; Karim, Faraizah Abdul; Mohammad, Wan Mohd Zahiruddin Wan; Hussein, Adil

doi:10.1007/s12288-017-0879-8

Cited by 6 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although FV and FVIII are homologous coagulation cofactors, their B domain differs in man, as FV has 25 potential N-linked glycosylation sites, but FVIII has 18 ( 36 ). Some authors ( 37 ) hypothesize that this difference may explain why FV appears to be more heat stable than FVIII. However, even though the B domain constitutes ~40% of the FVIII molecule, and different regions of B domain might participate in expression, intracellular transport, secretion and stability to various degrees, the functional characteristics of its specific regions are still obscure and its function has not been well-characterized ( 38 ).…”

Section: Discussionmentioning

confidence: 99%

Hemostatic Activity of Canine Never-Frozen Liquid Plasma Collected for Transfusion

et al. 2022

View full text Add to dashboard Cite

This study measured the changes of hemostatic activity in liquid plasma (LP) over 7 days of storage. Five canine plasma units, divided into two aliquots were evaluated: one stored refrigerated at 2–6°C as never-frozen LP and one frozen at −18°C as fresh frozen plasma (FFP). Clotting times, coagulation activities of factor (F) V, VIII, X, XI, antithrombin (AT), and von Willebrand (vWF), fibrinogen and D-dimers (DD) content were assessed before storage (baseline value), and after 12, 24, 48 h and 7 days (D7) in LP stored refrigerated, and on day 7 in FFP. At baseline median values of all factor activity were greater than 80%, and for clotting times, AT, fibrinogen and DD content, were within the canine reference range. Some hemostatic parameters changed significantly over 7 days and at the end of storage in LP. However, median activities of FV, FVIII, FX and FXI, coagulation time, AT, fibrinogen and DD content remained within reference ranges at all time points. The only exception was for vWF which median activity was lower than reference range for all storage time points. Activity of FVIII was significant lower in LP at D7 when compared to activity in FFP, with values of 62 vs. 118%, respectively. DD content showed a median value higher than reference range in FFP at D7. Despite some statistically significant changes at the end of 7-day storage period, never-frozen LP maintained median factor activities >80% for most factors. The clinical impact of the drop over time of vWF activity is unknown.

show abstract

Section: Discussionmentioning

confidence: 99%

Hemostatic Activity of Canine Never-Frozen Liquid Plasma Collected for Transfusion

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The other coagulation factors mean activities were [ 50% on day 5 of storage in both groups. They concluded that the coagulation factors activities were still sufficient to treat bleeding patients as the activities were more than 30%, as 30% is the minimal level of coagulation factors required for adequate haemostasis [11].…”

Section: Discussionmentioning

confidence: 99%

A Preliminary Study on Coagulation Parameters and Sterility of Thawed Refrozen Fresh Frozen Plasma

Draman¹,

Yousuf

Aziz

et al. 2019

Indian J Hematol Blood Transfus

View full text Add to dashboard Cite

Thawed fresh frozen plasma (FFP) if not used within 6 h, may have to be discarded due to the risk of contamination and uncertainty about its quality. The main objective of this study was to evaluate the levels of coagulation Factor II (FII), Factor VIII (FVIII), fibrinogen and bacterial growth in thawed refrozen FFP. Thirty FFP samples were collected from healthy donors. FFP were thawed in water bath at 37 °C for 20-25 min. Approximately 10 mL of plasma from each FFP unit was tested for FII, FVIII, fibrinogen and sterility. The thawed FFP units were then kept at 4 °C for 6 h before being refrozen and stored at -20 °C. Two weeks later, the refrozen FFP were thawed again and representative samples were analysed as before. There was a significant decline in the mean FVIII level, from 155.77% to 85.6% at second thaw. The mean FII level increased significantly from 74.9% to 82%, whereas the mean fibrinogen level fell from 3.34g/L to 3.28 g/L, but the decline was not statistically significant. There was no bacterial contamination in all samples at both time points. Refrozen plasma may be considered as an alternative to the storage of thawed unused FFP provided they are kept in a controlled environment to reduce wastage. These thawed refrozen FFP can be used later in bleeding cases like other FFP as the levels of FVIII are still within the standard haematology range (0.5-2 IU/mL) and above the minimal level of 30% coagulation factors required for adequate haemostasis.

show abstract

“…FFP is frozen at −18 °C within 8 h of collection, which extends the shelf life to 1 year, but it can be further extended to 7 years when the FFP is stored at −65 °C [ 110 ].…”

Section: Transfusion Reactionsmentioning

confidence: 99%

Blood Transfusion Reactions—A Comprehensive Review of the Literature including a Swiss Perspective

Ackfeld

Schmutz

Guéchi

et al. 2022

JCM

View full text Add to dashboard Cite

Blood transfusions have been the cornerstone of life support since the introduction of the ABO classification in the 20th century. The physiologic goal is to restore adequate tissue oxygenation when the demand exceeds the offer. Although it can be a life-saving therapy, blood transfusions can lead to serious adverse effects, and it is essential that physicians remain up to date with the current literature and are aware of the pathophysiology, initial management and risks of each type of transfusion reaction. We aim to provide a structured overview of the pathophysiology, clinical presentation, diagnostic approach and management of acute transfusion reactions based on the literature available in 2022. The numbers of blood transfusions, transfusion reactions and the reporting rate of transfusion reactions differ between countries in Europe. The most frequent transfusion reactions in 2020 were alloimmunizations, febrile non-hemolytic transfusion reactions and allergic transfusion reactions. Transfusion-related acute lung injury, transfusion-associated circulatory overload and septic transfusion reactions were less frequent. Furthermore, the COVID-19 pandemic has challenged the healthcare system with decreasing blood donations and blood supplies, as well as rising concerns within the medical community but also in patients about blood safety and transfusion reactions in COVID-19 patients. The best way to prevent transfusion reactions is to avoid unnecessary blood transfusions and maintain a transfusion-restrictive strategy. Any symptom occurring within 24 h of a blood transfusion should be considered a transfusion reaction and referred to the hemovigilance reporting system. The initial management of blood transfusion reactions requires early identification, immediate interruption of the transfusion, early consultation of the hematologic and ICU departments and fluid resuscitation.

show abstract

Coagulation Factor Activities Changes Over 5 Days in Thawed Fresh Frozen Plasma Stored at Different Initial Storage Temperatures

Cited by 6 publications

References 25 publications

Hemostatic Activity of Canine Never-Frozen Liquid Plasma Collected for Transfusion

Hemostatic Activity of Canine Never-Frozen Liquid Plasma Collected for Transfusion

A Preliminary Study on Coagulation Parameters and Sterility of Thawed Refrozen Fresh Frozen Plasma

Blood Transfusion Reactions—A Comprehensive Review of the Literature including a Swiss Perspective

Contact Info

Product

Resources

About