Vicenin-2: a potential radiosensitizer of non-small cell lung cancer cells

Abstract

Non-small cell lung cancer (NSCLC) is a major form of cancer and is resistant to chemo- and radio-therapy. Vicenin-2 (VCN-2) is a flavonoid obtained from Ocimum sanctum L. and it has been reported to have radioprotective and anti-cancer properties. This study was conducted to check for the radiosensitizing potential of VCN-2 in the NSCLC cell line, NCI-H23. NCI-H23 cells were exposed to VCN-2 singularly, and to X-rays with and without prior VCN-2 treatment. Cytotoxicity assay, cell proliferation assay, caspase-3 activity assay, DNA fragmentation assay and Western blotting for Rad50, MMP-2 and p21 were performed to investigate the radiosensitizing properties of VCN-2. Fibroblast survival assay was performed using HEK293T cells to check for any adverse effects of VCN-2 on normal fibroblast cell line. VCN-2 singularly and in combination with radiation reduced the surviving cancer cells, increased caspase-3 activity, increased DNA fragmentation, increased the levels of Rad50 and lowered levels of MMP-2 and p21 proteins while being non-toxic and radioprotective to the fibroblast cells. VCN-2 showed a potent radiosensitizing property while also showing a chemotherapeutic property against NSCLC cell line NCI-H23.

Vicenin‐2 acts as a radiosensitizer of the non‐small cell lung cancer by lowering Akt expression

“…3a, d, e, and h). This decrease in total Akt is carried out by caspase-3 mediated cleavage of Akt [61] which again corresponded with the observed increase in both caspase-3 activity level in our previous study (25) and its mRNA expression level in the VCN-2-treated group in combination with radiation ( Fig. 5f).…”

“…We conducted this study to check the effect of VCN‐2, a flavonoid of Ocimum sanctum L. , on the expression of Akt and modulation of the activity of its downstream targets that could result in favor of a pro‐apoptotic environment in the NSCLC cell line NCI‐H23, singularly and in combination with radiation. VCN‐2 has been shown to be a flavonoid with anticancer properties and potential radiosensitizing abilities . Thus, we carried out this study to further investigate the mechanism of action of VCN‐2 vis‐à‐vis its radiosensetizing potential.…”

Anticancer activity of Vicenin‐2 against 7,12 dimethylbenz[a]anthracene‐induced buccal pouch carcinoma in hamsters

“…[ 13 ] Oral treatment of Vicenin‐2 in prostate cancer rodent models inhibits blood vessel formation (angiogenesis), associated with tumor regression. [ 14 ] However, the anticancer potential of Vicenin‐2 against the 7,12‐dimethylbenz[ a ]anthracene (DMBA)‐provoked oral carcinogenesis has not been studied yet. Hence, in this study, we planned to explore the chemopreventive effect of Vicenin‐2 in DMBA‐induced buccal pouch carcinogenesis in hamsters via inhibition of proliferating cell nuclear antigen (PCNA).…”

Potential of Vicenin-2 in Chitosan Encapsulated Basil (Ocimum sanctum Linn.) Leaf Extract as A Therapeutic Alternative Medicine for Non-small cell carcinoma lung cancer

“…(Zari et al, 2021) Vicenin-2 secara efektif dapat menginduksi anti-proliferatif, anti-angiogenik, menghambat efek proapoptosis dan jalur kanker EGFR/Akt/mTOR/p70S6K bersama dengan penurunan c-Myc, cyclin D1, cyclin B1, CDK4, PCNA dan hTERT (Joseph and Nair, 2013). Vicenin-2 terbukti memiliki sifat radioprotektif dan anti kanker terhadap non-small cell carcinoma lung cancer (Baruah et al, 2018). Dengan radioterapi 6 dan 8 Gy, memiliki penurunan sel kanker yang signifikan jika ditambah dengan Vincenin-2.…”

“…Vicenin-2 (VCN-2) is a flavonoid extracted from the basil (Ocimum sanctum Linn.) that has been found to possess radioprotective and anticancer effects (Baruah et al, 2018). Basil (Ocimum sanctum Linn) is a widespread plant in Asia, particularly in Southeast Asia and South Asia, including Indonesia.…”