3-Acetylpyridine-induced ataxic-like motor impairments are associated with plastic changes in the Purkinje cells of the rat cerebellum

González-Tapia, David; Vázquez-Hernández, Nallely; Urmeneta-Ortiz, F.; Navidad-Hernandez, N.; Lazo-Yepez, M.; Tejeda-Martínez, A.R.; Flores-Soto, M.E.; González-Burgos, Ignacio

doi:10.1016/j.nrl.2021.09.005

Cited by 2 publications

(1 citation statement)

References 46 publications

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“…The neurotoxin 3-AP is a nicotinamide antagonist targeting the inferior olivary nucleus and has been extensively used to generate animal models of cerebellar ataxia. A study has shown that 3-AP reduced the density of dendritic spines of Purkinje cells and decreased the expressions of AMPA and PSD-95 in the cerebellar cortex, suggesting that 3-AP-induced ataxic-like motor impairments associated with an alteration in the morphology and connectivity of Purkinje cells [ 37 ]. In the present study, we initially used a dose of 65 mg/kg 3-AP based on other previous studies [ 38 , 39 ], but none of the rats survived within a day after intraperitoneal injection.…”

Section: Discussionmentioning

confidence: 99%

Hericium erinaceus Promotes Anti-Inflammatory Effects and Regulation of Metabolites in an Animal Model of Cerebellar Ataxia

Chau

Chong

Jin

et al. 2023

IJMS

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Cerebellar ataxia is a neurodegenerative disorder with no definitive treatment. Although previous study demonstrated the neuroprotective effects of Hericium erinaceus (H.E.), the mechanisms of H.E. treatment on the neuroinflammatory response, neurotransmission, and related metabolites remain largely unknown. We demonstrated that 3-AP rats treated with 25 mg/kg H.E. extracts had improved motor coordination and balance in the accelerated rotarod and rod tests. We showed that the H.E. treatment upregulated the expression of Tgfb1, Tgfb2, and Smad3 genes to levels comparable to those in the non-3-AP control group. Interestingly, we also observed a significant correlation between Tgfb2 gene expression and rod test performance in the 3-AP saline group, but not in the non-3-AP control or H.E.+3-AP groups, indicating a relationship between Tgfb2 gene expression and motor balance in the 3-AP rat model. Additionally, we also found that the H.E. treatment increased mitochondrial COX-IV protein expression and normalized dopamine-serotonin neurotransmission and metabolite levels in the cerebellum of the H.E.+3-AP group compared to the 3-AP saline group. In conclusion, our findings suggest that the H.E. treatment improved motor function in the 3-AP rat model, which was potentially mediated through neuroprotective mechanisms involving TGFB2-Smad3 signaling via normalization of neurotransmission and metabolic pathways.

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Section: Discussionmentioning

confidence: 99%

Hericium erinaceus Promotes Anti-Inflammatory Effects and Regulation of Metabolites in an Animal Model of Cerebellar Ataxia

Chau

Chong

Jin

et al. 2023

IJMS

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Does Moringa Oleifera Protect the Brain Against 3-Acetylpyridine-Induced Cerebellar Ataxia?

Hassan,

Sabra,

Farghaly

et al. 2024

Preprint

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There is no treatment for some neurological conditions, like cerebellar ataxia (CA). Moringa Oleifera (MO) has been revealed to have neuroprotective properties, but little is known about how it could protect against CA. In this study, we studied the neuroprotective effects of MO in an animal model of CA induced by 3-acetylpyridine (3-AP), which showed deficits in balance and motor coordination. Although cerebellar neuroinflammatory responses are evident in CA, it is yet unclear how neuroinflammation might influence CA. Here, we investigate whether MO, which has anti-inflammatory, antioxidant, and neuroprotective qualities, can help with cerebellar neurodegeneration and locomotor activity deficits. Four groups of 24 adult male rats were divided into four equal groups. The control group received saline orally, the MO group received MO extract orally, the 3-AP group was injected with 3-AP, and the 3-AP + MO combined group received both 3-AP and MO for four weeks. The animals underwent a motor coordination test on the experiment’s first and last days. At the end of the experiment, the animals were euthanized, the cerebellums were dissected, and they were then subjected to standard biochemical, histological, and immunohistochemical studies. The combined group showed remarkable improvement in the CA. The cerebellar neurodegeneration and declination of locomotor activity had improved. Our findings imply that MO may protect against the CA degenerative condition and improve cerebellar function.

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3-Acetylpyridine-induced ataxic-like motor impairments are associated with plastic changes in the Purkinje cells of the rat cerebellum

Cited by 2 publications

References 46 publications

Hericium erinaceus Promotes Anti-Inflammatory Effects and Regulation of Metabolites in an Animal Model of Cerebellar Ataxia

Hericium erinaceus Promotes Anti-Inflammatory Effects and Regulation of Metabolites in an Animal Model of Cerebellar Ataxia

Does Moringa Oleifera Protect the Brain Against 3-Acetylpyridine-Induced Cerebellar Ataxia?

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