3,4-Dicaffeoylquinic Acid, a Major Constituent of Brazilian Propolis, Increases TRAIL Expression and Extends the Lifetimes of Mice Infected with the Influenza A Virus

Abstract

Brazilian green propolis water extract (PWE) and its chemical components, caffeoylquinic acids, such as 3,4-dicaffeoylquinic acid (3,4-diCQA), act against the influenza A virus (IAV) without influencing the viral components. Here, we evaluated the anti-IAV activities of these compounds in vivo. PWE or PEE (Brazilian green propolis ethanol extract) at a dose of 200 mg/kg was orally administered to Balb/c mice that had been inoculated with IAV strain A/WSN/33. The lifetimes of the PWE-treated mice were significantly extended compared to the untreated mice. Moreover, oral administration of 3,4-diCQA, a constituent of PWE, at a dose of 50 mg/kg had a stronger effect than PWE itself. We found that the amount of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) mRNA in the mice that were administered 3,4-diCQA was significantly increased compared to the control group, while H1N1 hemagglutinin (HA) mRNA was slightly decreased. These data indicate that PWE, PEE or 3,4-diCQA possesses a novel and unique mechanism of anti-influenza viral activity, that is, enhancing viral clearance by increasing TRAIL.

Comparative Chemistry of Propolis from Eight Brazilian Localities

“…For example, in Table 3 several substances are caffeoylquinic acids, comprising of 1–3 residues of caffeic acid ( 57 , 60 , 62 , 65 , 66 , 75 , 84 , 86 , 87 , 93 , 100 , 104 , 106 , and 107 ). Such compounds are characteristic and abundant constituents of green propolis, obtained ideally from aqueous extracts [45, 46]. In some cases, one residue of caffeic acid is replaced by other phenylpropanoid, such as ferulic acid ( 61 , 67 , 68 , and 113 ).…”

Identification and quantification of phenolic compounds in Vitex negundo L. var. cannabifolia (Siebold et Zucc.) Hand.-Mazz. using liquid chromatography combined with quadrupole time-of-flight and triple quadrupole mass spectrometers

“…Combined with the aforementioned findings, chlorogenic acid, agnuside, isochlorogenic acid A, and isochlorogenic acid C may be selected as suitable marker compounds for the quality control of this plant because of their various strong bioactivities [13,14,[32][33][34][35]. However, further validation experiments are necessary.…”

Antioxidant Activity and Phenolic Acids Profiles of Artemisia Selengensis Turcz Extracted with Various Methods by HPLC-QTOF-MS/MS

“…; Takemura et al . ). Thus, AST extract might be explored as a promising resource of natural antioxidants and health care products due to the high antioxidant potential of CQA derivates.…”

“…In addition, phenolic acids have received increasing attention because of their appreciable health benefits, such as anti-inflammatory, antimutagenicity, antioxidant, and neuroprotective effect and so forth, by reducing the reactive oxygen and nitrogen species gener-ated during the complications of many chronic diseases. (Islam et al 2003;Kim et al 2005;Takemura et al 2011). Thus, AST extract might be explored as a promising resource of natural antioxidants and health care products due to the high antioxidant potential of CQA derivates.…”