The evaluation of E. faecalis colonies dissolution ability of sodium hypochlorite in microenvironment by a novel device

Sun, Xiaoqiang; Li, Shaorong; Wang, Shujing; Luo, Chunxiong; Hou, Benxiang

doi:10.1007/s10544-018-0279-3

Cited by 7 publications

(7 citation statements)

References 31 publications

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“…The samples without sinus tracts were characterized by Microbacterium and Enterococcus . Enterococcus , a genus of gram-positive, facultatively anaerobic bacteria, was much more likely to be found in cases of persistent infections than in primary infections due to its ability to invade the dentinal tubules and highly resistant to antimicrobial strategies ( Sun et al., 2009 ; Murad et al., 2014 ; Keskin et al., 2017 ; Bouillaguet et al., 2018 ; Sun et al., 2018 ; Barbosa-Ribeiro et al., 2021 ; Gomes et al., 2021 ; Zhang et al., 2021 ). In the present study, Enterococcus was consistently detected in all of the samples but with a low mean proportion of OTUs.…”

Section: Discussionmentioning

confidence: 99%

Microbial Communities in the Extraradicular and Intraradicular Infections Associated With Persistent Apical Periodontitis

Sun

Yang

Nie

et al. 2022

Front. Cell. Infect. Microbiol.

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Microorganisms in the complex root canal system and the extraradicular regions, including the periapical lesions and extraradicular biofilm may cause root canal treatment failures. However, few studies described the difference between the intraradicular and extraradicular infections from the same tooth associated with persistent apical periodontitis. This study aimed to characterize the microbiome present in the root canal, extraradicular biofilm, and periapical lesions associated with persistent apical periodontitis. The microbial communities in the root canal, extraradicular biofilm, and periapical lesions were investigated by Illumina high-throughput sequencing using Illumina Hiseq 2500 platform. The dominant phyla in the extraradicular and intraradicular infections associated with persistent apical periodontitis were Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria, and the genera Fusobacterium, Morganella, Porphyromonas, Streptococcus, and Bifidobacterium dominated across all samples. Although extraradicular infection sites showed higher OTU richness and β-diversity compared to intraradicular samples, the occurrence of sinus tract rather than the sampling sites demarcated the microbial communities in the infections associated with persistent apical periodontitis. PERMANOVA analysis confirmed that the samples with or without sinus tracts contained significantly different microbial communities. Porphyromonas, Eubacterium, Treponema, and Phocaeicola were found in significantly higher levels with sinus tracts, whilst Microbacterium and Enterococcus were more abundant in samples without sinus tracts. In conclusion, diverse bacteria were detected in both intraradicular and extraradicular infections associated with persistent apical periodontitis, which might be influenced by the occurrence of the sinus tract. The results may provide new insight into the pathogenesis of persistent apical periodontitis.

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Section: Discussionmentioning

confidence: 99%

Microbial Communities in the Extraradicular and Intraradicular Infections Associated With Persistent Apical Periodontitis

Sun

Yang

Nie

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Furthermore, several recent studies have shown that Enterococcus faecalis, a grampositive facultative anaerobe, plays the most important role in secondary and persistent root canal and periapical infections. [8][9][10][11] One of the reasons is the ability of this bacterium to form a biofilm, which is a highly sheltered structure containing other microorganisms. The formation of bacterial clusters on the walls of infected root canals-in other words-biofilm formation, has been reported, although detailed descriptions were not provided.…”

Section: Palavras-chavementioning

confidence: 99%

“…The formation of bacterial clusters on the walls of infected root canals-in other words-biofilm formation, has been reported, although detailed descriptions were not provided. [11,12] It is therefore necessary to completely eliminate this bacterium from infected root canals for successful endodontic treatment, [13] and irrigation during chemomechanical preparation and mechanical cleaning has been reported to play an important role in this bacterial elimination procedure. [14,15] Mechanical agitation has been found to increase the efficacy of irrigation solutions.…”

Section: Palavras-chavementioning

confidence: 99%

“…[8,30,31] We selected E. faecalis for our study because its elimination is extremely challenging, it penetrates deep into the dental tissues, it can survive even in negative conditions and it is resistant to intracanal medicaments. [11,32,33] Irrigation performed with mechanical cleaning and shaping of root canals constitutes one of the most important stages of root canal treatment. [6] However, conventional chemomechanical preparation methods appear adequate for the total elimination of microorganisms from root canals.…”

Section: Experimental Groupsmentioning

confidence: 99%

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Comparison of different final irrigant agitation techniques for the removal of Enterococcus faecalis biofilms from root canals: an in vitro study

Güneç

Haznedaroğlu

Külekçi

et al. 2018

BDS

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Objective: The aim of this in vitro study was to compare the effectiveness of different final irrigant agitation techniques in the removal of Enterococcus faecalis biofilms from root canals. Material and Methods: In total, the root canals of 85 extracted single-rooted human maxillary incisors teeth were prepared using the Revo-S system to a 40/06 size. The apical foramen of each tooth was sealed by light-cured resin composite material to obstruct bacterial leakage. The specimens were sterilized in an autoclave at 121°C for 15 min and stored until further use. All teeth except five (negative control group) were inoculated with Enterococcus faecalis and incubated in a CO2 chamber at 37°C for 7 days; the trypticase soy broth was changed every 2 days. For the determination of possible biofilm formation, five of the 80 teeth were randomly selected as a positive control group; one tooth of positive control group was analysed for biofilm development by scanning electron microscope (SEM) and these teeth received no final irrigant agitation procedure. Then, the remaining 75 teeth were randomly divided into five test groups (n=15 each) and were sequentially irrigated with 5% sodium hypochlorite (NaOCl), 17% ethylenediaminetetraacetic acid and 5% NaOCl. Following each irrigant application, different final irrigant agitation techniques were introduced for 60 s (3×20-s sessions). Group 1 received manual–dynamic agitation, group 2 received passive ultrasonic agitation (PUI), group 3 received EndoActivator agitation, group 4 received photon-initiated photoacoustic streaming (PIPS) with the Er:YAG laser and group 5 received conventional syringe irrigation. Colony-forming units (CFUs) were counted in samples from the positive control and test groups. Data were analysed using Kruskal–Wallis and post-hoc Mann–Whitney U multiple comparison tests. Results: E. faecalis elimination was significantly better in the experimental groups than in the positive control groups (p < 0.001). Manual–dynamic agitation and conventional syringe irrigation, with no significant differences between the two groups. Conclusion: Essentially, CFU reduction was significantly greater in the PUI, EndoActivator and PIPS groups than in the manual–dynamic agitation and conventional syringe irrigation groups (p <0.001), with no significant differences among the former three groups.KeywordsEnterococcus faecalis; Irrigant agitation; Irrigant solutions; Photon-initiated photoacoustic streaming; Passive ultrasonic irrigation.

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“…Disinfecting a root canal includes removal of necrotic tissues, accumulated debris and eliminating the number of bacteria (2). Upon infections, Enterococcus faecalis has been considered the main and most resistant microorganism in root canal system (3). Mechanical preparation alone is insufficient to clean the root canal system (4).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial effect of sodium hypochlorite gel on Enterococcus faecalis in endodontics: A systematic review

2020

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Aim: The aim of this systematic review was to investigate the in vitro antibacterial effect of sodium hypochlorite gel on Enterococcus faecalis during root canal treatment. Methods: An electronic databases (PubMed, ScienceDirect, Google Scholar, CENTRAL, Health Advance) was performed (last updated was 1st April 2019). The reference lists of the included studies were hand-searched. Randomized in vitro studies that compared sodium hypochlorite gel to solution as a root canal irrigant were included. No limitation on publication date. Results: Out of 20741 articles, only two studies with 116 human teeth were included in this review. The studies showed 50% high risk of bias, and 50% medium risk of bias for the included studies. Both sodium hypochlorite gel and solution had some bacterial growth inhibition over E. faecalis. The heterogeneity in methodology of the included studies and the lack of high evidence led to contradictory results. However, sodium hypochlorite solution was better in enhancing the antibacterial effect according to the robust study. Conclusion: There is insufficient reliable evidence about the antibacterial effect of sodium hypochlorite gel on enterococcus faecalis. Although the robust study included in this review revealed that sodium hypochlorite gel is less efficient than the solution as a root canal irrigant, further studies are needed to consider the most effective type, concentration, duration and treatment protocols in enhancing the antibacterial effect of sodium hypochlorite. How to cite this article: Al Nesser S, Bshara N, Adıgüzel Ö. Antibacterial effect of sodium hypochlorite gel on Enterococcus faecalis in endodontics: A systematic review. Int Dent Res 2020;10(1):22-29. https://doi.org/10.5577/intdentres.2020.vol10.no1.3 Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

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The evaluation of E. faecalis colonies dissolution ability of sodium hypochlorite in microenvironment by a novel device

Cited by 7 publications

References 31 publications

Microbial Communities in the Extraradicular and Intraradicular Infections Associated With Persistent Apical Periodontitis

Microbial Communities in the Extraradicular and Intraradicular Infections Associated With Persistent Apical Periodontitis

Comparison of different final irrigant agitation techniques for the removal of Enterococcus faecalis biofilms from root canals: an in vitro study

Antibacterial effect of sodium hypochlorite gel on Enterococcus faecalis in endodontics: A systematic review

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