2018
DOI: 10.1002/bit.26515
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Abstract: The conversion of lignocellulose-rich biomass to bio-based chemicals and higher order fuels remains a grand challenge, as single-microbe approaches often cannot drive both deconstruction and chemical production steps. In contrast, consortia based bioprocessing leverages the strengths of different microbes to distribute metabolic loads and achieve process synergy, product diversity, and bolster yields. Here, we describe a biphasic fermentation scheme that combines the lignocellulolytic action of anaerobic fungi… Show more

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Cited by 52 publications
(72 citation statements)
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References 55 publications
(88 reference statements)
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“…The transcriptome of P. finnis , N. californiae , and A. robustus has been previously assembled with reference to corresponding genomes . Here, fungal RNA was isolated from A. robustus and N. californiae during exponential growth (pressure ~3–8 psig) using a Qiagen RNEasy Mini Kit (Qiagen, Valencia, CA) following the manufacturer's instructions for plants and fungi.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The transcriptome of P. finnis , N. californiae , and A. robustus has been previously assembled with reference to corresponding genomes . Here, fungal RNA was isolated from A. robustus and N. californiae during exponential growth (pressure ~3–8 psig) using a Qiagen RNEasy Mini Kit (Qiagen, Valencia, CA) following the manufacturer's instructions for plants and fungi.…”
Section: Methodsmentioning
confidence: 99%
“…Although sequencing has revealed a treasure trove of biomass‐degrading enzymes, nearly 70% of protein encoding genes in some gut fungal strains still lack functional annotation and may be involved in biomass degradation . Furthermore, emerging technologies seek to employ isolated gut fungi directly in anaerobic bioprocessing, though it is unknown how culture conditions influence enzyme secretion, especially when fungi are deployed in anaerobic consortia …”
Section: Introductionmentioning
confidence: 99%
“…The impact of fungi in the rumen ecosystem was already demonstrated in the early 1990s by Gordon and Phillips who reported a significant decrease in fiber digestion within the rumen after anaerobic fungi had been removed by the administration of fungicides 22 . The importance of rumen fungi for biomass degradation has since the been supported by in vivo studies [23][24][25] , and recently reinforced in transcriptome studies revealing that the fungi express a range of CAZymes when grown on different carbon sources 9,26 . Although enzymes of fungal origin have been regularly explored for their remarkable capacity to degrade lignocellulosic fiber 12,27,28 , their functional role in native anaerobic habitats and within the biomass-degrading enzyme repertoire of the rumen microbiome remains unclear.…”
Section: Introductionmentioning
confidence: 97%
“…Recent studies have also shed light on the viral rumen population and although work in this area is still nascent, it suggests that the rumen virome modulates carbon cycling within the rumen ecosystem through cell lysis or re-programming of the metabolism of the host microbiome 5,7,8 . Anaerobic rumen ciliate protozoa and fungi have largely remained recalcitrant to both cultivation and molecular exploration efforts 9 , and although recent cultivation efforts have provided important insight into the lifestyle and enzymatic capacity 4,10 , their quantitative metabolic contributions to the greater rumen ecosystem are still unclear.…”
Section: Introductionmentioning
confidence: 99%
“…These organisms play a pivotal role in the digestion of plant biomass in herbivores, due to the physical and chemical way in which they degrade plant biomass [14]. Recent work highlights the bounty of biotechnological applications of these fungi [15]. Given that these organisms typically thrive in consortia, it is desirable to emulate nature to unlock their potential for bioconversion of unpretreated lignocellulose.…”
Section: Introductionmentioning
confidence: 99%