Imaging phospholipid conformational disorder and packing in giant multilamellar liposome by confocal Raman microspectroscopy

Noothalapati, Hemanth; Iwasaki, Keita; Yoshimoto, Chikako; Yoshikiyo, Keisuke; Nishikawa, Tomoe; Ando, Masahiro; Hamaguchi, Hiro O.; Yamamoto, Takashi

doi:10.1016/j.saa.2017.06.060

Cited by 22 publications

(13 citation statements)

References 38 publications

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“…The I 1090 / I 1129 ratio implies the combination of intermolecular and interchain disorder. Further, the intensity ratio of 1090/1064 cm −1 was used to probe the extent of trans/gauche isomerization 56 . In this study, the structure of PC was analyzed by differential spectra to subtract background caused by protein, and the disorder of lipid chains was expressed through I 1090 / I 1064 and I 1090 / I 1129 , as shown in Table 5.…”

Section: Resultsmentioning

confidence: 99%

Stability Mechanism of Two Soybean Protein-Phosphatidylcholine Nanoemulsion Preparation Methods from a Structural Perspective: A Raman Spectroscopy Analysis

Zhu

et al. 2019

Sci Rep

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Ultrasound treatment and high-pressure homogenization were used to prepare soybean protein (SP)-phosphatidylcholine (PC) nanoemulsions in this study. Nanoemulsions prepared by high-pressure homogenization were more stable. The structural changes of SP and PC under ultrasound treatment and high-pressure homogenization treatment were investigated by Raman spectroscopy. It could be concluded that ultrasound and high-pressure homogenization treatments increased both the content of α-helix and unordered structure but decreased that of β-structures of SP, while the interaction between SP and PC decreased α-helix content and also reduced unordered structure and β-sheet structure. Ultrasound treatment and high-pressure homogenization exposed more tryptophan and tyrosine residues to promote hydrophobic interaction between SP and PC, which was beneficial for stabilizing the nanoemulsion. The SP-PC interaction exerted a more significant effect on side chain structure than those observed under ultrasound treatment and high-pressure homogenization. The dominant g-g-t vibrational mode of the disulfide bond of soybean protein was not appreciably changed by the two preparations. High-pressure homogenization increased the disorder of lipid chains of PC, promoting SP-PC interaction and thereby increasing the stability of the nanoemulsion. The structural change provided a theoretical basis for preparation of two nanoemulsions.

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Section: Resultsmentioning

confidence: 99%

Stability Mechanism of Two Soybean Protein-Phosphatidylcholine Nanoemulsion Preparation Methods from a Structural Perspective: A Raman Spectroscopy Analysis

Zhu

et al. 2019

Sci Rep

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“…This may be relevant for the distribution of disordered phases in true bilayer and monolayer systems. Imaging of phospholipid liposomes and phase‐separated Langmuir–Blodgett films have shown nanoscale domains corresponding to differences in alkyl chain packing. Supercells in the ab ‐plane that may be related to nanodomains have been proposed to explain the neutron and X‐ray diffraction data in Langmuir–Blodgett films…”

Section: Resultsmentioning

confidence: 99%

“…Future work will examine phase transitions in medium to long chain chlorotriazine derivatives (C > 10), some of which (C12, C16) are already known to form mesophases . The minimum least squares approach can then be applied to Raman imaging data of biologically relevant bilayer systems for quantifying the structural phases within nanodomains.…”

Section: Resultsmentioning

confidence: 99%

“…Alkyl chain packing and disorder is of continued interest across a range of fields, including biology where disorder may have an impact on ligand recognition processes in lipid bilayers, self‐assembly of liquid‐crystal materials, crystal engineering, and packing density in multilamellar liposomes . Understanding structural chemistry of biological membrane lipid bilayers is challenged by the difficulty of crystallising such systems, and there is an ongoing demand for spectroscopic approaches, such as solution nuclear magnetic resonance (NMR) and vibrational spectroscopy, for studying bilayer systems that are difficult or impossible to crystallise.…”

Section: Introductionmentioning

confidence: 99%

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Quantifying alkyl chain disorder in crystalline models of lipid bilayers using Raman spectroscopy

Rich

Bhattacharyya

Aldilla

et al. 2018

J Raman Spectroscopy

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Crystalline bis‐N‐alkyl‐chlorotriazines 2–6 can be considered multilayers of lipid bilayers comprising a rigid hydrogen‐bonded tape in the polar layer separated by a lipid region of small to medium‐length alkyl chains. The persistent ordering of these tapes acts as a scaffold in the solid state enabling measurement of temperature‐induced conformational changes in the “lipid phase” using X‐ray crystallography. The alkyl chains in these structures are loosely packed but generally ordered at low temperature, undergoing a slow transition to a disordered structure clearly observed in the Raman spectrum. The longer‐chain derivatives 4–6 show a distinct Raman spectrum for the ordered and disordered crystallographic phases enabling a least squares fitting at each temperature point. Calibration of the Raman fit values for 5 and 6 using powder X‐ray diffraction allows a quantification of each phase and highlights the unique response of each derivative with temperature. This approach for quantifying the onset of disorder and structural phase transitions should be relevant to a wide range of lipid‐based systems.

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“…The T m has an important effect on the structure of the biomembrane. When the phospholipids are below their specific T m , the individual phospholipids are closely packed, and they form a rigid ordered gel phase 13 , 14 . When the temperature gets close to the T m , the tightly packed phospholipids start to loosen up, resulting in more empty space between the phospholipids.…”

Section: Introductionmentioning

confidence: 99%

Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing

Chen

Duša

Witos

et al. 2018

Sci Rep

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Our study demonstrates that nanoplasmonic sensing (NPS) can be utilized for the determination of the phase transition temperature (Tm) of phospholipids. During the phase transition, the lipid bilayer undergoes a conformational change. Therefore, it is presumed that the Tm of phospholipids can be determined by detecting conformational changes in liposomes. The studied lipids included 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Liposomes in gel phase are immobilized onto silicon dioxide sensors and the sensor cell temperature is increased until passing the Tm of the lipid. The results show that, when the system temperature approaches the Tm, a drop of the NPS signal is observed. The breakpoints in the temperatures are 22.5 °C, 41.0 °C, and 55.5 °C for DMPC, DPPC, and DSPC, respectively. These values are very close to the theoretical Tm values, i.e., 24 °C, 41.4 °C, and 55 °C for DMPC, DPPC, and DSPC, respectively. Our studies prove that the NPS methodology is a simple and valuable tool for the determination of the Tm of phospholipids.

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Imaging phospholipid conformational disorder and packing in giant multilamellar liposome by confocal Raman microspectroscopy

Cited by 22 publications

References 38 publications

Stability Mechanism of Two Soybean Protein-Phosphatidylcholine Nanoemulsion Preparation Methods from a Structural Perspective: A Raman Spectroscopy Analysis

Stability Mechanism of Two Soybean Protein-Phosphatidylcholine Nanoemulsion Preparation Methods from a Structural Perspective: A Raman Spectroscopy Analysis

Quantifying alkyl chain disorder in crystalline models of lipid bilayers using Raman spectroscopy

Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing

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