IgG Avidity: an Important Serologic Marker for the Diagnosis of Tick-Borne Encephalitis Virus Infection

Vilibić-Čavlek, Tatjana; Barbić, Ljubo; Stevanović, Vladimir; Petrović, Goranka; Mlinarić‐Galinović, Gordana

doi:10.5604/17331331.1197285

Cited by 15 publications

(5 citation statements)

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“…Avidity maturation of an antibody represents an increasing affinity interaction between antibodies and antigens (Ferreira-Júnior et al ., 2012). Considering AI > 60% as high avidity (Vilibic-Cavlek et al ., 2016), throughout the stages of the chicken's immune response, only high-avidity antibodies were produced. A high mean AI of 78.30% was observed, therefore demonstrating that A. suum -immunized hens produce large amounts of high-avidity polyclonal-specific IgY antibodies just one week after the first immunization.…”

Section: Discussionmentioning

confidence: 99%

Anti-Ascaris suumimmunoglobulin Y as a novel biotechnological tool for the diagnosis of human ascariasis

et al. 2019

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Human ascariasis is a neglected tropical disease of great relevance to public health and is considered the most frequent helminthiasis in poor regions. Accurately diagnosing this parasite has been challenging due to limitations of current diagnostic methods. Immunoglobulin Y (IgY) technology is a very effective alternative for the production of highly specific and profitable antibodies. This study aimed to produce and apply anti-Ascaris suum IgY antibodies in the immunodiagnosis of human ascariasis. Five immunizations comprising total saline extract from A. suum adult life forms were given at 14-day intervals to Gallus gallus domesticus hens of the Isa Brown line. Eggs and blood samples were collected weekly and fortnightly, respectively, to monitor the production of antibodies. The specificity of antibodies was confirmed by dot-blot, kinetic enzyme-linked immunosorbent assay (ELISA), avidity ELISA, immunoblotting and indirect immunofluorescence antibody tests. The application for disease diagnosis was performed through the detection of immune complexes in human serum samples by sandwich ELISA. Peaks of IgY anti-A. suum production occurred at weeks 6 and 8. IgY showed high avidity levels after the second dose of immunization, ranging from 64% to 93%, with a mean avidity index of 78.30%. Purified IgY recognized 12 bands of proteins from A. suum saline extract. Eggs, the uterine portion and cuticles of A. suum female adult are reactive in immunofluorescence. The detection of immune complexes showed diagnostic values of 80% sensitivity and 90% specificity. In conclusion, specific IgY have been shown to be a potential immunodiagnostic tool with promising future applications in human ascariasis.

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Section: Discussionmentioning

confidence: 99%

Anti-Ascaris suumimmunoglobulin Y as a novel biotechnological tool for the diagnosis of human ascariasis

et al. 2019

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“…For TBE, IgG avidity testing may be useful to rule out false-positive results due to cross-reactive IgM antibodies induced by other flaviviruses or in cases of atypical antibody responses, e.g. when IgM antibodies are persistently elevated past the acute phase of infection (Vilibic-Cavlek et al 2016 ). For Lyme borreliosis, a recently developed IgG avidity Western blot has shown promising first results to identify disease stage (Mavin et al 2018 ).…”

Section: Commonly Used Diagnostic Methods For Tbds In Human and Veterinary Medicinementioning

confidence: 99%

Tick-borne zoonoses and commonly used diagnostic methods in human and veterinary medicine

et al. 2021

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Around the world, human health and animal health are closely linked in terms of the One Health concept by ticks acting as vectors for zoonotic pathogens. Animals do not only maintain tick cycles but can either be clinically affected by the same tick-borne pathogens as humans and/or play a role as reservoirs or sentinel pathogen hosts. However, the relevance of different tick-borne diseases (TBDs) may vary in human vs. veterinary medicine, which is consequently reflected by the availability of human vs. veterinary diagnostic tests. Yet, as TBDs gain importance in both fields and rare zoonotic pathogens, such as Babesia spp., are increasingly identified as causes of human disease, a One Health approach regarding development of new diagnostic tools may lead to synergistic benefits. This review gives an overview on zoonotic protozoan, bacterial and viral tick-borne pathogens worldwide, discusses commonly used diagnostic techniques for TBDs, and compares commercial availability of diagnostic tests for humans vs. domestic animals, using Germany as an example, with the aim of highlighting existing gaps and opportunities for collaboration in a One Health framework.

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“…Vë liau an ti kû -nø kie kis su ma þë ja, bet ið lie ka vi sam gy ve ni mui ir ap sau go nuo rein fek ci jos [25,28]. IgG avi dið ku mo (ry ðio stip ru mo tarp an ti kû no ir an ti ge no) nu sta ty mo tes tas tai ko mas pacien tams, ku riems nu sta to mas ati pi nis an ti kû nø at sa kas ir bû ti na di fe ren ci juo ti tarp esa mos ar bu vu sios in fek ci jos ir imu ni te to po vak ci na vi mo [29]. In tra te ka liai spe ci fi niai an ti kû nai nu sta to mi vë liau ne gu se ru me, vi du ti nið kai 9-10 li gos die nà.…”

Section: Diagnostikaunclassified

Epidemiology, clinical characteristics, diagnosis and vaccination of tick-borne encephalitis: an overview

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Valaikienė³

2018

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ÁVADASEr kiø per ne ða mos li gos yra di dë jan ti vi suo me nës svei katos pro ble ma. Yra þi no ma, kad er kës ga li per neð ti iki 16 skir tin gø in fek ci niø su kë lë jø [1]. Er ki nis en ce fa litas (EE) yra vie na ið svar biau siø vi ru si niø þmo gaus cen trinës ner vø sis te mos in fek ci jø, ku rià su ke lia er ki nio en ce fali to vi ru sas (EEV). Þmo nëms daþ niau ke liau jant, EE ta po glo ba lia pro ble ma. To dël, áta riant cen tri nës ner vø sis te mos in fek ci jà, EE tu ri bû ti átrauk tas á di fe ren ci nae diag nos ti kà ne tik as me nims, gy ve nan tiems en de mi në se zo no se, bet ir kelia vu siems á di des nës ri zi kos re gio nus. Ri zi ka su sirg ti EE ke lio nës me tu pri klau so nuo se zo no, ap sau gos prie mo niø nau do ji mo si, ne pa ste ri zuo to pie no pro duk tø var to ji mo [2]. Nors li gos pre ven ci jai yra su kur tos efek ty vios ir sau gios vak ci nos, pas ta rai siais de ðimt me èiais ste bi mas di de lis serga mu mo pa di dë ji mas vi sa me pa sau ly je, ypaè Lie tu vo je Lie tu va yra en de mi nis er kiø pla ti na mø li gø re gio nas dël di de lio mið kin gu mo (treè da lá Lie tu vos te ri to ri jos su da ro mið kai), di dë jan èio ne dir ba mø þe miø plo to ir er këms veistis pa lan kiø kli ma to sà ly gø [5]. EPIDEMIOLOGIJAEEV pri klau so Flavivirus gen èiai, Fla vi vi ri dae ðei mai. Erkiø per ne ða mi ir þmo gui pa vo jin gi vi ru sai yra dar va di nami ti bo vi ru sais (angl. tick borne vi rus). Vi ru sai, to kie kaip Oms ko he mo ra gi nës karð ti nës vi ru sas Si bi re, Ky a sa nu ro Fo res to li gos vi ru sas In di jo je, Alk hur ma vi ru sas Sau do Ara bi jo je, yra gi mi nin gi EEV. Lou pin go li gos vi ru su Jung ti në je Ka ra lys të je uþ si kre èia avys, ta èiau re gist ruo ti pa na ðûs á EE su sir gi mai þmo nëms, dir ban tiems su vi ru su uþ krës tais gy vû nais (la bo ran tams, ve te ri na rams, më si ninkams). Jung ti në se Ame ri kos Vals ti jo se ir Ru si jo je pas taruo ju me tu ëmë dau gë ti Po wa sa no vi ru so su kel tø su sir gimø [6]. Pir mà kar tà EEV bu vo ið skir tas 1937 m. [6]. EEV ið gy ven ti rei ka lin gi du skir tin gi ðei mi nin kai -er kës ir stubu ri niai gy vû nai. Uþ si krë tu sios er kës per duo da vi ru sà svei koms er këms ir stu bu ri niams gy vû nams, ku rie rei kalin gi uþ krës ti EEV svei kas er kes jø mai ti ni mo si me tu [7]. San trau ka. Pas ta ruo sius me tus re gist ruo ja mas di dë jan tis er ki nio en ce fa li to (EE) su sir gi mø skai èius ir ste bi mas er ki nio en ce fa li to vi ru so (EEV) pli ti mas á nau jas te ri to ri jas. Prie þas tys ðiai pa sau li nei ten den ci jai -po pu lia rë jan tis po il sis gam to je, au gan tys tu ris tø srau tai á aukð tos ri zi kos zo nas, taip pat ge rë jan ti EE diag nos ti ka. Lie tu va yra vie na ið ke liø Eu ro pos ða liø, kurio se uþ fik suo ja ma dau giau sia EE at ve jø. 2016 m. Lie tu vo je re gist ruo ta dvi gu bai dau giau EE at ve jø nei 2015 m. EEV þmo nëms ga li su kel ti sun kiø neu ro lo gi niø simp to mø ir lie ka mø -jø reið ki niø. Diag nos ti kà sun ki na spe...

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IgG Avidity: an Important Serologic Marker for the Diagnosis of Tick-Borne Encephalitis Virus Infection

Cited by 15 publications

References 13 publications

Anti-Ascaris suumimmunoglobulin Y as a novel biotechnological tool for the diagnosis of human ascariasis

Anti-Ascaris suumimmunoglobulin Y as a novel biotechnological tool for the diagnosis of human ascariasis

Tick-borne zoonoses and commonly used diagnostic methods in human and veterinary medicine

Epidemiology, clinical characteristics, diagnosis and vaccination of tick-borne encephalitis: an overview

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