Agatoxin-like peptides in the neuroendocrine system of the honey bee and other insects

Sturm, Sebastian; Ramesh, Divya; Brockmann, Axel; Neupert, Susanne; Predel, Reinhard

doi:10.1016/j.jprot.2015.11.021

Cited by 29 publications

(26 citation statements)

References 49 publications

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“…As more insect genomes and transcriptomes were sequenced [31,42,83,190,194] it became apparent that Drosophila has one of the smallest insect neuropeptidomes owing to the fact that it has lost several neuropeptide signaling systems which are present in basal insects like the migratory locust Locusta migratoria and the termite Zootermopsis nevadensis [31]. Neuropeptide systems lost in Drosophila include AKH/corazonin related peptide (ACP) [195], agatoxin-like peptide (ALP) [196], allatostatin-CCC [197], allatotropin [37], calcitonin, elevenin, neuroparsin, SMYamide [31], thyrotropin-releasing hormone (TRH) [66], tryptopyrokinin [198], neuroparsins [199] and inotocin (related to vertebrate oxytocin/vasopressin) [159]. The lack of powerful genetic tools in other insect models has hampered investigations into these signaling systems.…”

Section: Neuropeptides Lost In Drosophilamentioning

confidence: 99%

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Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior

Nässel

Zandawala

2019

Preprint

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This review focuses on neuropeptides and peptide hormones, the largest and most diverse class of neuroactive substances, known in Drosophila and other animals to play roles in almost all aspects of daily life, as well as in developmental processes. We provide an update on novel neuropeptides and receptors identified in the last decade, and highlight progress in analysis of neuropeptide signaling in Drosophila. Especially exciting is the huge amount of work published on novel functions of neuropeptides and peptide hormones in Drosophila, largely due to the rapid developments of powerful genetic methods, imaging techniques and innovative assays. We critically discuss the roles of peptides in olfaction, taste, foraging, feeding, clock function/sleep, aggression, mating/reproduction, learning and other behaviors, as well as in regulation of development, growth, metabolic and water homeostasis, stress responses, fecundity, and lifespan. We furthermore provide novel information on neuropeptide distribution and organization of peptidergic systems, as well as the phylogenetic relations between Drosophila neuropeptides and those of other phyla, including mammals. As will be shown, neuropeptide signaling is phylogenetically ancient, and not only are the structures of the peptides, precursors and receptors conserved over evolution, but also many functions of neuropeptide signaling in physiology and behavior.

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Section: Neuropeptides Lost In Drosophilamentioning

confidence: 99%

“…Hence, it is involved in post-ecdysis related behavior. The functions of ALP, allatostatin-CCC, calcitonin, SMYamide and TRH in insects are still unknown [31,194,196,197].…”

Section: Neuropeptides Lost In Drosophilamentioning

confidence: 99%

Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior

Nässel

Zandawala

2019

Preprint

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“…Recently, the neuropeptide RY amide in Drosophila has been thought to lose most of its physiological significance (Veenstra & Khammassi, 2017), while its receptor was still completely functional (Collin et al, 2011). Even though, the Apis AKH has been detected by mass spectrometry (Sturm et al, 2016), and the AKHR was still found to respond to the physiological change regardless of the carbohydrate or lipid metabolism (Woodard et al, 2011;Wang et al, 2012), which indicated that the activation and desensitization of AKHR and its physiological functions might be more complicated in response to the eusocial life.…”

Section: Discussionmentioning

confidence: 99%

“…In honey bees (Apis mellifera), the transcription of AKH and AKHR has been detected in head and fat body, and the expression of AKHR was upregulated in response to the downregulation of both vitellogenin and ultraspiracle via RNA interference (Wang et al, 2012). The existence of additional TATA box in the promoter region of the Akh gene resulted in the diminished expression of AKH (Veenstra et al, 2012), which made it difficult to detect mature AKH via mass spectrometry (Boerjan et al, 2010;Sturm et al, 2016). What is more, the activation and desensitization mechanisms of AKHR become more attractive.…”

Section: Introductionmentioning

confidence: 99%

Prediction of the post-translational modifications of adipokinetic hormone receptors from solitary to eusocial bees

et al. 2018

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Adipokinetic hormone receptor (AKHR) was regarded as the crucial regulator of lipid consuming, but now has been renewed as a pluripotent neuropeptide G protein-coupled receptor. It has been identified in all sequenced bee genomes from the solitary to the eusocial. In the current study, we try to clarify the transitions of AKHR on lipid utilization and other potential functions from solitary to eusocial bees. The results showed that the AKHRs were divided into different groups based on their social complexity approximately. Nevertheless, the critical motifs and tertiary structures were highly conserved. As to the post-translational modifications, the eusocial possessed more phosphorylation residues and modification patterns, which might be due to the necessity of more diverse functions. These results suggest that AKHRs are highly conserved on both primary motifs and tertiary structures, but more flexible on posttranslational modifications so as to accommodate to more complicated eusocial life.

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“…It is currently unknown what are the pharmacological targets or receptors of ShK-like1 and ShK-like2 and whether these targets are shared between fish and sea anemones. Toxin-like molecules have been reported in vertebrate and insect brains (43)(44)(45)(46), but in many cases their sequence similarity to toxins is very low making convergent evolution a realistic scenario. One previously reported example is Agatoxin-like peptides in the insect neuroendocrine system where the sequence similarity to the corresponding spider toxin is quite high (45).…”

Section: Partial Functional Specialization Of Shk-like1 Compared To Smentioning

confidence: 99%

Toxin-like neuropeptides in the sea anemoneNematostellaunravel recruitment from the nervous system to venom

Sachkova

Landau

Surm

et al. 2020

Preprint

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The sea anemone Nematostella vectensis (Anthozoa, Cnidaria) is a powerful model system for characterizing the evolution of genes functioning in venom and nervous systems. Despite being an example for evolutionary novelty, the evolutionary origin of most toxins remains unknown.Here we report the first bona fide case of protein recruitment from the cnidarian nervous to venom system. The ShK-like1 peptide has ShKT cysteine motif, is lethal for fish larvae and packaged into nematocysts, the cnidarian venom-producing stinging capsules. Thus, ShK-like1 is a toxic venom component. Its paralog, ShK-like2, is a neuropeptide localized to neurons and is involved in development. Interestingly, both peptides exhibit similarities in their functional activities: both of them provoke contraction in Nematostella polyps and are toxic to fish. Because ShK-like2 but not ShK-like1 is conserved throughout sea anemone phylogeny, we conclude that the two paralogs originated due to a Nematostella-specific duplication of a ShK-like2 ancestor, a neuropeptideencoding gene, followed by diversification and partial functional specialization. Strikingly, ShK-like2 is represented by two gene isoforms controlled by alternative promoters conferring regulatory flexibility throughout development. Additionally, we characterized the expression patterns of four other peptides with structural similarities to studied venom components, and revealed their unexpected neuronal localization. Thus, we employed genomics, transcriptomics and functional approaches to reveal one new venom component, five neuropeptides with two different cysteine motifs and an evolutionary pathway from nervous to venom system in Cnidaria.

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Agatoxin-like peptides in the neuroendocrine system of the honey bee and other insects

Cited by 29 publications

References 49 publications

Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior

Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior

Prediction of the post-translational modifications of adipokinetic hormone receptors from solitary to eusocial bees

Toxin-like neuropeptides in the sea anemoneNematostellaunravel recruitment from the nervous system to venom

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