Sterilization of polydimethylsiloxane surface with Chinese herb extract: a new antibiotic mechanism of chlorogenic acid

Ren, Song; Wu, Ming; Guo, Jiayu; Zhang, Wang; Liu, Xiaohan; Sun, Lili; Hołyst, Robert; Hou, Sen; Fang, Yongchun; Feng, Xisheng

doi:10.1038/srep10464

Cited by 18 publications

(13 citation statements)

References 38 publications

(47 reference statements)

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“…(2) CGA molecules display strong polarity and can bind to large molecules, such as lipids, on the surface of bacteria to change the permeability of the bacterial membrane, resulting in the leakage of the contents [ 14 , 17 ]. (3) CGA may inhibit bacterial flagella synthesis, reduce the number of flagella, and thus reduce the bacterial clustering effect [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Chlorogenic Acid on Bacillus subtilis Based on Metabolomics

Liang

Zhang

et al. 2020

Molecules

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Chlorogenic acid (CGA), a natural phenolic compound, is an important bioactive compound, and its antibacterial activity has been widely concerned, but its antibacterial mechanism remains largely unknown. Protein leakage and the solution exosmosis conductivity of Bacillus subtilis 24434 (B. subtilis) reportedly display no noticeable differences before and after CGA treatment. The bacterial cells treated with CGA displayed a consistently smooth surface under the electron microscope, indicating that CGA cannot directly disrupt bacterial membranes. However, CGA induced a significant decrease in the intracellular adenosine triphosphate (ATP) concentration, possibly by affecting the material and energy metabolism or cell-signaling transduction. Furthermore, metabolomic results indicated that CGA stress had a bacteriostatic effect by inducing the intracellular metabolic imbalance of the tricarboxylic acid (TCA) cycle and glycolysis, leading to metabolic disorder and death of B. subtilis. These findings improve the understanding of the complex action mechanisms of CGA antimicrobial activity and provide theoretical support for the application of CGA as a natural antibacterial agent.

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Section: Introductionmentioning

confidence: 99%

The Effect of Chlorogenic Acid on Bacillus subtilis Based on Metabolomics

Liang

Zhang

et al. 2020

Molecules

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“…Since E. coli DH5α has a high resistance to antibiotics6, we used E. coli DH5α as the Gram-negative bacteria to check the antibacterial effect of paeonol. We also used P. aeruginosa PAO1 as the Gram-positive bacteria in the study.…”

Section: Resultsmentioning

confidence: 99%

“…Various techniques have been developed for the sterilization of PDMS surface. PDMS surfaces coated with antibacterial materials, such as titania and chlorogenic acid, are reported to have good antibacterial activity56. However, these materials are usually either poorly effective against cancer cells or highly toxic to non-cancer mammalian cells.…”

mentioning

confidence: 99%

Antibacterial and anticancer PDMS surface for mammalian cell growth using the Chinese herb extract paeonol(4-methoxy-2-hydroxyacetophenone)

Jiao

Sun

Guo

et al. 2016

Sci Rep

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Polydimethylsiloxane (PDMS) is widely used as a cell culture platform to produce micro- and nano-technology based microdevices. However, the native PDMS surface is not suitable for cell adhesion and is always subject to bacterial pollution and cancer cell invasion. Coating the PDMS surface with antibacterial or anticancer materials often causes considerable harm to the non-cancer mammalian cells on it. We have developed a method to fabricate a biocompatible PDMS surface which not only promotes non-cancer mammalian cell growth but also has antibacterial and anticancer activities, by coating the PDMS surface with a Chinese herb extract, paeonol. Coating changes the wettability and the elemental composition of the PDMS surface. Molecular dynamic simulation indicates that the absorption of paeonol onto the PDMS surface is an energy favourable process. The paeonol-coated PDMS surface exhibits good antibacterial activity against both Gram-positive and Gram-negative bacteria. Moreover considerable antibacterial activity is maintained after the coated surface is rinsed or incubated in water. The coated PDMS surface inhibits bacterial growth on the contact surface and promotes non-cancer mammalian cell growth with low cell toxicity; meanwhile the growth of cancer cells is significantly inhibited. Our study will potentially guide PDMS surface modification approaches to produce biomedical devices.

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“…1) is one of the most available active substances widely distributed in not only lots of Chinese herbal medicine but also in various fruits and vegetables. It has been extensively demonstrated for wide-ranging physiological activities such as anti-obesity, 1) anti-diabetic, 2) antihypertension, 3) antimicrobial, 4) and anti-inflammatory activities. [5][6][7] Analyzing from the structure, CGA is the simple ester of caffeic acid with quinic acid, two common natural metabolites, which suggests that it has strong potential for chemical modification.…”

Section: Introductionmentioning

confidence: 99%

Structural Optimization of Caffeoyl Salicylate Scaffold as NO Production Inhibitors

Pan

Xia

et al. 2019

CHEMICAL & PHARMACEUTICAL BULLETIN

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Chlorogenic acid (CGA) has been considered as one of important active components in a number of medicinal herbs. Recently our group demonstrated that caffeoyl salicylate scaffold derived from CGA can be employed for the development of novel anti-inflammatory agents. The most active compound D104 can be a very promising starting point for the further structural optimization. A series of novel caffeoyl salicylate analogs were designed, synthesized, and evaluated by preliminary biological evaluation. The obtained results showed that the two compounds B12 and B13 can not only inhibit production of nitric oxide (NO) in RAW264.7 cells induced by lipopolysaccharides (LPS) effectively, but also have high safety in in vitro cytotoxic test, which could be comparable with D104. Molecular docking study on the peroxisome proliferatoractivated receptor γ (PPARγ) protein revealed that compounds B12 and B13 can follow the same binding mode with D104, and the carboxyl group of caffeoyl salicylate scaffold might play a key role in the interaction with protein target, which implied the carboxyl group should be retained in the further optimization.

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Sterilization of polydimethylsiloxane surface with Chinese herb extract: a new antibiotic mechanism of chlorogenic acid

Cited by 18 publications

References 38 publications

The Effect of Chlorogenic Acid on Bacillus subtilis Based on Metabolomics

The Effect of Chlorogenic Acid on Bacillus subtilis Based on Metabolomics

Antibacterial and anticancer PDMS surface for mammalian cell growth using the Chinese herb extract paeonol(4-methoxy-2-hydroxyacetophenone)

Structural Optimization of Caffeoyl Salicylate Scaffold as NO Production Inhibitors

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