Distribution and Development of Peripheral Glial Cells in the Human Fetal Cochlea

Locher, Heiko; Groot, John Cmj de; Iperen, Liesbeth van; Huisman, Margriet A.; Frijns, Johan H. M.; Lopes, Susana M. Chuva de Sousa

doi:10.1371/journal.pone.0088066

Cited by 33 publications

(36 citation statements)

References 59 publications

(79 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Staining for p75NTR is present as early as GW 5 in the human fetal cochleo-vestibular ganglion (Vega et al 1999) and also among the peripheral processes of the human fetal cochlea at GW 9 (Locher et al 2014). In the present study, immuno-staining for this receptor was seen from GW 9 onwards and finally extended as far as the inner pillar cells at GW 12.…”

Section: Discussionsupporting

confidence: 61%

Role of BDNF and neurotrophic receptors in human inner ear development

et al. 2017

View full text Add to dashboard Cite

The expression patterns of the neurotrophin, brain-derived neurotrophic factor, BDNF, and the neurotrophic receptors-p75NTR and Trk receptors-in the developing human fetal inner ear between the gestational weeks (GW) 9 to 12 are examined via in situ hybridization and immunohistochemistry. BDNF mRNA expression was highest in the cochlea at GW 9 but declined in the course of development. In contrast to embryonic murine specimens, a decline in BDNF expression from the apical to the basal turn of the cochlea could not be observed. p75NTR immunostaining was most prominent in the nerve fibers that penetrate into the sensory epithelia of the cochlea, the urticule and the saccule as gestational age progresses. TrkB and TrkC expression intensified towards GW 12, at which point the BDNF mRNA localization was at its lowest. TrkA expression was limited to fiber subpopulations of the facial nerve at GW 10. In the adult human inner ear, we observed BDNF mRNA expression in the apical poles of the cochlear hair cells and supporting cells, while in the adult human utricle, the expression was localized in the vestibular hair cells. We demonstrate the highly specific staining patterns of BDNF mRNA and its putative receptors over a developmental period in which multiple hearing disorders are manifested. Our findings suggest that BDNF and neurotrophin receptors are important players during early human inner ear development. In particular, they seem to be important for the survival of the afferent sensory neurons.

show abstract

Section: Discussionsupporting

confidence: 61%

Role of BDNF and neurotrophic receptors in human inner ear development

et al. 2017

View full text Add to dashboard Cite

show abstract

“…3(A)]. Previously, we have shown that SOX10+ cells are present in the spiral ganglion and throughout the cochlear epithelium (Locher et al, 2014). In this study, we also detected SOX10 expression in the presumptive melanocytes bordering the stria vascularis [Fig.…”

Section: Resultsmentioning

confidence: 86%

“…3(B)]. We recently observed additional expression throughout the human fetal cochlear duct epithelium (Locher et al, 2013) and in all peripheral glial cells (Locher et al, 2014). Although it is likely that melanocytes play a major role in SNHL in WS or KS due to SOX10 mutations, it cannot be excluded that other SOX10+ cell types in the cochlea are involved as well.…”

Section: Discussionmentioning

confidence: 90%

“…This implies that the endocochlear potential cannot be generated at this stage and, therefore, that the human fetus at W18 is still unable to hear. Although W20 has been proposed by us (Locher et al, 2013; Locher et al, 2014) and others (Pujol et al, 1991; Pujol and Lavigne‐Rebillard, 1995; Bibas et al, 2008) as the onset of human hearing based on the maturation of the organ of Corti and the cochlear nerve, it is more likely that hearing commences a few weeks later, which is in line with otoacoustic emission measurements (Chabert et al, 2006) and auditory brainstem responses in preterm infants (Lary et al, 1985; van Straaten et al, 2001). …”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development of the stria vascularis and potassium regulation in the human fetal cochlea: Insights into hereditary sensorineural hearing loss

Locher

Groot

Iperen

et al. 2015

Developmental Neurobiology

Self Cite

View full text Add to dashboard Cite

Sensorineural hearing loss (SNHL) is one of the most common congenital disorders in humans, afflicting one in every thousand newborns. The majority is of heritable origin and can be divided in syndromic and nonsyndromic forms. Knowledge of the expression profile of affected genes in the human fetal cochlea is limited, and as many of the gene mutations causing SNHL likely affect the stria vascularis or cochlear potassium homeostasis (both essential to hearing), a better insight into the embryological development of this organ is needed to understand SNHL etiologies. We present an investigation on the development of the stria vascularis in the human fetal cochlea between 9 and 18 weeks of gestation (W9–W18) and show the cochlear expression dynamics of key potassium‐regulating proteins. At W12, MITF+/SOX10+/KIT+ neural‐crest‐derived melanocytes migrated into the cochlea and penetrated the basement membrane of the lateral wall epithelium, developing into the intermediate cells of the stria vascularis. These melanocytes tightly integrated with Na+/K+‐ATPase‐positive marginal cells, which started to express KCNQ1 in their apical membrane at W16. At W18, KCNJ10 and gap junction proteins GJB2/CX26 and GJB6/CX30 were expressed in the cells in the outer sulcus, but not in the spiral ligament. Finally, we investigated GJA1/CX43 and GJE1/CX23 expression, and suggest that GJE1 presents a potential new SNHL associated locus. Our study helps to better understand human cochlear development, provides more insight into multiple forms of hereditary SNHL, and suggests that human hearing does not commence before the third trimester of pregnancy. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 75: 1219–1240, 2015

show abstract

“…This is consistent with S100B location in a number of other nervous system locations. For instance, S100B was found associated with ensheathing cells of the rat olfactory pathway during development [41], peripheral glial cells of the human fetal cochlea [42], satellite cells of sensory, sympathetic and enteric ganglia, and in supporting cells of the adrenal medulla, myelinating and non-myelinating Schwann cells [31]. Peripheral neurons express both S100A1 and B, while the dorsal root ganglion contained S100B in the human peripheral nervous system.…”

Section: Distribution Of S100bmentioning

confidence: 99%

Subcellular Localization of the “Classic” S100 Subunits in Vestibular End Organs of the Rat

Foster

2017

MOJAP

View full text Add to dashboard Cite

Using immunohistochemical techniques, the distribution of the "classic" S100 calcium binding proteins, α (A1) and β (B), was determined in the mammalian vestibular end organs. An affinity-purified polyclonal antibody which recognized both the A1 and B subunits and two monoclonal antibodies specific for either the A1 or the B subunit were used in this study. S100A1 was localized specifically to the basal region of the sensory epithelium (saccular macula, cristae ampullaris and utricular macula) when a cross-linking fixative (4% paraformaldehyde) was utilized. Using a fixation protocol including paraformaldehyde and a precipitating fixative revealed an additional localization of S100A1 to the apical region of the saccular macula, cristae ampullaris and utricular macula. This indicates the presence of two populations of S100A1 in these cells: a cytosolic (soluble) component; and a membrane-bound fraction. In contrast, S100B was localized to the layer of the vestibular nerve fibers. Immunoreactivity for S100B was not observed in the sensory epithelium of the saccular or utricular macula or in the cristae ampullaris. The preferential distribution of S100B to the nerve fibers and S100A1 to cells of the sensory epithelium, in two forms (cytosolic and membranebound), may be important in the functional roles of S100 proteins in the vestibular sensory end organs.

show abstract

Distribution and Development of Peripheral Glial Cells in the Human Fetal Cochlea

Cited by 33 publications

References 59 publications

Role of BDNF and neurotrophic receptors in human inner ear development

Role of BDNF and neurotrophic receptors in human inner ear development

Development of the stria vascularis and potassium regulation in the human fetal cochlea: Insights into hereditary sensorineural hearing loss

Subcellular Localization of the “Classic” S100 Subunits in Vestibular End Organs of the Rat

Contact Info

Product

Resources

About