2014
DOI: 10.1371/journal.pone.0086731
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Abstract: BackgroundThe complexity of plant cell walls creates many challenges for microbial decomposition. Clostridium phytofermentans, an anaerobic bacterium isolated from forest soil, directly breaks down and utilizes many plant cell wall carbohydrates. The objective of this research is to understand constraints on rates of plant decomposition by Clostridium phytofermentans and identify molecular mechanisms that may overcome these limitations.ResultsExperimental evolution via repeated serial transfers during exponent… Show more

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Cited by 2 publications
(2 citation statements)
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“…The only reliably‐annotated sugar transporter in CAG:83 and CAG:241 is a Cut1 family ABC transporter, two membrane subunits of which are present in both genomes (99% identity between CAG:83 and CAG:241), with homologs of over 70% identity in the other members of the Oscillibacter clade. Cut1 family transporters are known to be involved in uptake of oligosaccharides, glycerol‐phosphate and polyols (Schneider, ), and although they have not been well studied in Clostridia, genetic evidence supports their role in plant fibre utilization by Clostridium phytofermentans (Mukherjee et al ., ). Unfortunately, because of the broad substrate specificity of the Cut1 family it is currently impossible to predict the substrates of these transporters in Oscillospira and other Oscillibacter clade organisms.…”
Section: Introductionmentioning
confidence: 97%
“…The only reliably‐annotated sugar transporter in CAG:83 and CAG:241 is a Cut1 family ABC transporter, two membrane subunits of which are present in both genomes (99% identity between CAG:83 and CAG:241), with homologs of over 70% identity in the other members of the Oscillibacter clade. Cut1 family transporters are known to be involved in uptake of oligosaccharides, glycerol‐phosphate and polyols (Schneider, ), and although they have not been well studied in Clostridia, genetic evidence supports their role in plant fibre utilization by Clostridium phytofermentans (Mukherjee et al ., ). Unfortunately, because of the broad substrate specificity of the Cut1 family it is currently impossible to predict the substrates of these transporters in Oscillospira and other Oscillibacter clade organisms.…”
Section: Introductionmentioning
confidence: 97%
“…Recently, improvement of C. phytofermentans' growth on cellobiose, cellulose, and xylan by experimental evolution yielded strains that also produced ethanol more quickly (41). The genome sequence of the ET strain presented here suggests other novel approaches to potentially improve ethanol resistance and production.…”
Section: Adhe Activitiesmentioning
confidence: 90%