24-Epibrassinolide enhances 5-ALA-induced anthocyanin and flavonol accumulation in calli of ‘Fuji’ apple flesh

Zheng, Jie; An, Yuyan; Wang, Liangju

doi:10.1007/s11240-018-1418-5

Cited by 33 publications

(41 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Heme oxygenase‐1 (HO‐1) promotes anthocyanin accumulation in radish hypocotyls (Liu et al ). Among many species of fruits, such as apples (Feng et al , Zheng et al , ) or litchi (Feng et al ), ALA can rapidly induce anthocyanin and flavonol accumulation. Therefore, it is reasonable to deduce that heme is an important metabolic derivative of ALA and may be involved in H 2 O 2 cell signal generation after treatment with exogenous ALA. We have obtained evidence on the signal pathway of ALA in improving salt tolerance of plants, which will be reported soon.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide as a mediator of 5‐aminolevulinic acid‐induced Na⁺ retention in roots for improving salt tolerance of strawberries

et al. 2019

Physiologia Plantarum

View full text Add to dashboard Cite

To explore the mechanisms of 5-aminolevulinic acid (ALA)-improved plant salt tolerance, strawberries (Fragaria × ananassa Duch. cv. 'Benihoppe') were treated with 10 mg l −1 ALA under 100 mmol l −1 NaCl stress. We found that the amount of Na + increased in the roots but decreased in the leaves. Laser scanning confocal microscopy (LSCM) observations showed that ALA-induced roots had more Na + accumulation than NaCl alone. Measurement of the xylem sap revealed that ALA repressed Na + concentrations to a large extent. The electron microprobe X-ray assay also confirmed ALA-induced Na + retention in roots. qRT-PCR showed that ALA upregulated the gene expressions of SOS1 (encoding a plasma membrane Na + /H + antiporter), NHX1 (encoding a vacuolar Na + /H + antiporter) and HKT1 (encoding a protein of high-affinity K + uptake), which are associated with Na + exclusion in the roots, Na + sequestration in vacuoles and Na + unloading from the xylem vessels to the parenchyma cells, respectively. Furthermore, we found that ALA treatment reduced the H 2 O 2 content in the leaves but increased it in the roots. The exogenous H 2 O 2 promoted plant growth, increased root Na + retention and stimulated the gene expressions of NHX1, SOS1 and HKT1. Diphenyleneiodonium (DPI), an inhibitor of H 2 O 2 generation, suppressed the effects of ALA or H 2 O 2 on Na + retention, gene expressions and salt tolerance. Therefore, we propose that ALA induces H 2 O 2 accumulation in roots, which mediates Na + transporter gene expression and more Na + retention in roots, thereby improving plant salt tolerance.Abbreviations -ALA, 5-aminolevulinic acid; ROS, reactive oxygen species; DPI, diphenyleneiodonium. † These authors equally contributed to this work leads to Na + toxicity, osmotic stress and nutrient deficiencies, with Na + toxicity being the primary cause of plant growth restriction (Zhu 2002). Many plants possess specific mechanisms to alleviate Na + toxicity, such as reducing Na + uptake, promoting Na + efflux or vacuolar sequestration (Maathuis et al. 2014). In the plasma membrane of Arabidopsis thaliana (Arabidopsis), the Na + /H + Physiol. Plant. 167, 2019

show abstract

Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide as a mediator of 5‐aminolevulinic acid‐induced Na⁺ retention in roots for improving salt tolerance of strawberries

et al. 2019

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…In plants, ALA, a natural plant growth regulator, has attracted increasing attention due to its various physiological roles and great application potential in agriculture and horticulture (Wang et al , Akram and Ashraf , Feng et al , An et al , , Zheng et al , An et al , Zheng et al ). We previously demonstrated a new role of ALA in regulating stomatal movement and revealed that H 2 O 2 signaling is crucial in ALA‐regulated stomatal movement (An et al ).…”

Section: Discussionmentioning

confidence: 99%

“…It has been recognized as a vital plant growth regulator due to its various roles in several key physiological processes such as promoting plant growth and conferring abiotic stress tolerance in plants , Niu et al 2017, Sun et al 2017, Akram et al 2018, Liu et al 2018, Wu et al 2018. Increasing attention has been paid to ALA because of its environmental-friendly Abbreviations -ABA, abscisic acid; ALA, 5-aminolevulinic acid; AsA, ascorbic acid; BR, brassinosteroid; CalA, calyculin A; CAT, catalase; DPI, diphenylene iodonium; GFP-TUA6, green fluorescent protein-tagged -tubulin 6 transgenic Arabidopsis; H 2 O 2 , hydrogen peroxide; MeJA, methyl jasmonate; MS, Murashige and Skoog; MTs, microtubules; OA, okadaic acid; OE, overexpression; PP2A, type 2A protein phosphatase; PP2A-C2, C2 subunit of type 2A protein phosphatase; PP2A-C2-OE, PP2A-C2 overexpression; PPFD, photosynthetic photo flux density; ROS, reactive oxygen species; VBT, vinblastine. characteristic and great application potential in agriculture (Wang et al 2003, Akram andAshraf 2013) and horticulture (Feng et al 2016, An et al 2016b, Zheng et al 2017, Zheng et al 2018. Our recent studies suggested a new role of ALA, regulating stomatal movement (An et al 2016a(An et al , 2016c.…”

mentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

PP2A and microtubules function in 5‐aminolevulinic acid‐mediated H₂O₂ signaling in Arabidopsis guard cells

Xiong

Shu

et al. 2019

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

5‐aminolevulinic acid (ALA), a plant growth regulator with great application potential in agriculture and horticulture, induces stomatal opening and inhibits stomatal closure by decreasing guard cell H2O2. However, the mechanisms behind ALA‐decreased H2O2 in guard cells are not fully understood. Here, using type 2A protein phosphatase (PP2A) inhibitors, microtubule‐stabilizing/disrupting drugs and green fluorescent protein‐tagged α‐tubulin 6 transgenic Arabidopsis (GFP‐TUA6), we find that PP2A and cortical microtubules (MTs) are involved in ALA‐regulated stomatal movement. Then, we analyze stomatal responses of Arabidopsis overexpressing C2 catalytic subunit of PP2A (PP2A‐C2) and pp2a‐c2 mutant to ALA and abscisic acid (ABA) under both light and dark conditions, and show that PP2A‐C2 participates in ALA‐induced stomatal movement. Furthermore, using pharmacological methods and confocal studies, we reveal that PP2A and MTs function upstream and downstream, respectively, of H2O2 in guard cell signaling. Finally, we demonstrate the role of H2O2‐mediated microtubule arrangement in ALA inhibiting ABA‐induced stomatal closure. Our findings indicate that MTs regulated by PP2A‐mediated H2O2 decreasing play an important role in ALA guard cell signaling, revealing new insights into stomatal movement regulation.

show abstract

“…Total anthocyanin contents were measured according to Zheng et al [74]. The fig peels, apple peels, and apple calli were taken and incubated in 1% (v/v) HCl-methanol for 24 h at room temperature in the dark.…”

Section: Determination Of Anthocyanin Contentsmentioning

confidence: 99%

Transcriptomic Analysis of Ficus carica Peels with a Focus on the Key Genes for Anthocyanin Biosynthesis

Wang

2020

IJMS

Self Cite

View full text Add to dashboard Cite

Fig (Ficus carica L.), a deciduous fruit tree of the Moraceae, provides ingredients for human health such as anthocyanins. However, little information is available on its molecular structure. In this study, the fig peels in the yellow (Y) and red (R) stages were used for transcriptomic analyses. Comparing the R with the Y stage, we obtained 6224 differentially expressed genes, specifically, anthocyanin-related genes including five CHS, three CHI, three DFR, three ANS, two UFGT and seven R2R3-MYB genes. Furthermore, three anthocyanin biosynthetic genes, i.e., FcCHS1, FcCHI1 and FcDFR1, and two R2R3-MYB genes, i.e., FcMYB21 and FcMYB123, were cloned; sequences analysis and their molecular characteristics indicated their important roles in fig anthocyanin biosynthesis. Heterologous expression of FcMYB21 and FcMYB123 significantly promoted anthocyanin accumulation in both apple fruits and calli, further suggesting their regulatory roles in fig coloration. These findings provide novel insights into the molecular mechanisms behind fig anthocyanin biosynthesis and coloration, facilitating the genetic improvement of high-anthocyanin cultivars and other horticultural traits in fig fruits.

show abstract

24-Epibrassinolide enhances 5-ALA-induced anthocyanin and flavonol accumulation in calli of ‘Fuji’ apple flesh

Cited by 33 publications

References 60 publications

Hydrogen peroxide as a mediator of 5‐aminolevulinic acid‐induced Na⁺ retention in roots for improving salt tolerance of strawberries

Hydrogen peroxide as a mediator of 5‐aminolevulinic acid‐induced Na⁺ retention in roots for improving salt tolerance of strawberries

PP2A and microtubules function in 5‐aminolevulinic acid‐mediated H₂O₂ signaling in Arabidopsis guard cells

Transcriptomic Analysis of Ficus carica Peels with a Focus on the Key Genes for Anthocyanin Biosynthesis

Contact Info

Product

Resources

About

24-Epibrassinolide enhances 5-ALA-induced anthocyanin and flavonol accumulation in calli of ‘Fuji’ apple flesh

Cited by 33 publications

References 60 publications

Hydrogen peroxide as a mediator of 5‐aminolevulinic acid‐induced Na+ retention in roots for improving salt tolerance of strawberries

Hydrogen peroxide as a mediator of 5‐aminolevulinic acid‐induced Na+ retention in roots for improving salt tolerance of strawberries

PP2A and microtubules function in 5‐aminolevulinic acid‐mediated H2O2 signaling in Arabidopsis guard cells

Transcriptomic Analysis of Ficus carica Peels with a Focus on the Key Genes for Anthocyanin Biosynthesis

Contact Info

Product

Resources

About

Hydrogen peroxide as a mediator of 5‐aminolevulinic acid‐induced Na⁺ retention in roots for improving salt tolerance of strawberries

Hydrogen peroxide as a mediator of 5‐aminolevulinic acid‐induced Na⁺ retention in roots for improving salt tolerance of strawberries

PP2A and microtubules function in 5‐aminolevulinic acid‐mediated H₂O₂ signaling in Arabidopsis guard cells