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Parrot, Sandrine; Bert, Lionel; Mouly-Badina, Laurence; Sauvinet, Valérie; Colussi-Mas, Joyce; Lambás‐Señas, Laura; Robert, F.; Bouilloux, Jean-Pierre; Suaud-Chagny, Marie-Françoise; Denoroy, Luc; Renaud, Bernard

doi:10.1023/a:1025009221285

Cited by 55 publications

(20 citation statements)

References 51 publications

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“…By using CE-LIFD, we were able to improve the time resolution of the biochemical determinations and to analyze the spinal microdialysates collected every minute. This makes possible a monitoring with a better time resolution, although higher sampling rates obtained in the rat have not yet been reached in humans [7,8]. …”

Section: Discussionmentioning

confidence: 99%

“…However, since large volumes of samples are necessary with that technique, microdialysis samples had to be collected at best every 5 min. As capillary electrophoresis (CE) is a technique able to analyze nanovolume samples with low limits of detection, it is especially appropriate for the monitoring of neurotransmitters with a higher temporal resolution microdialysis, as already demonstrated in rodents [7,8].…”

Section: Introductionmentioning

confidence: 99%

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Capillary electrophoresis combined with microdialysis in the human spinal cord: A new tool for monitoring rapid peroperative changes in amino acid neurotransmitters within the dorsal horn

Parrot

Sauvinet

Xavier³

et al. 2004

Electrophoresis

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A method originally developed for the separation of the three neurotransmitters gamma-aminobutyric acid (GABA), glutamate (Glu) and L-aspartate (L-Asp) in microdialysis samples from rat brain (Sauvinet et al., Electrophoresis 2003, 24, 3187-3196) was applied to human spinal dialysates obtained during peroperative microdialysis from patients undergoing surgery against chronic pain. Molecules were tagged on their primary amine function with the fluorogene agent, naphthalene-2,3-dicarboxaldehyde (NDA), and, after separation by capillary electrophoresis (CE, 75 mmol/L borate buffer, pH 9.2, containing 70 mmol/L sodium dodecyl sulfate and 10 mmol/L hydroxypropyl-beta-cyclodextrin, + 25 kV voltage), were detected by laser-induced fluorescence detection (LIFD) using a 442 nm helium-cadmium laser. The complete method, including microdialysis sampling and analysis by CE-LIFD, has been validated for the analysis of human spinal microdialysates. The analytical detection limits were 1, 3.7 and 17 nmol/L for GABA, Glu and L-Asp respectively. This method allows an accurate measurement of the three amino acid neurotransmitters during an in vivo monitoring performed as rapidly as every minute in the human spinal dorsal horn. In addition, the effect of a brief peroperative electrical stimulation of the dorsal rootlets was investigated. The results obtained illustrate the advantages of combining microdialysis with CE-LIFD for studying neurotransmitters with such a high sampling rate.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis combined with microdialysis in the human spinal cord: A new tool for monitoring rapid peroperative changes in amino acid neurotransmitters within the dorsal horn

Parrot

Sauvinet

Xavier³

et al. 2004

Electrophoresis

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“…Similarly, CE (CE/LIF) has been widely used for the study of amino acids [15,16], catecholamines [17] as well as peptides and proteins ( [18], for a review see [19]). …”

Section: Introductionmentioning

confidence: 99%

HPLC-fluorescence detection and MEKC-LIF detection for the study of amino acids and catecholamines labelled with naphthalene-2,3-dicarboxyaldehyde

Siri¹,

Lacroix

Garrigues

et al. 2006

Electrophoresis

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Naphthalene-2,3-dicarboxyaldehyde (NDA) is commonly used for detection of primary amines in conjunction with their separation with HPLC and CE. The fluorescence of the derivatives can be measured by a conventional fluorometer or via LIF. NDA is a reactive dye, which can replace o-phthaldehyde (OPA) and provides for derivatives which are considerably more stable than OPA derivatives. In addition, NDA can be used to derivatize primary amines at concentrations as low as 100 pM. In this work, HPLC/fluorescence and MEKC/LIF experiments were performed to separate/detect six neuroactive compounds, the amino acids, Gly, Glu, Asp, gamma-aminobutyric acid (GABA) and the catecholamines, dopamine and noradrenaline. The two methods were compared in terms of performance of separation. The amino acids can be separated in HPLC in less than 30 min and an identical separation is obtained in CE using MEKC and lithium salts with greater resolution (the number of theoretical plates was approximately 5000 for HPLC and 200 000 for MEKC). The lowest detected concentration was in the range of 0.1 nM for CE/LIF. The presence of a high salt concentration does not affect the separation of the samples. Examples of the analysis of microdialysate samples as well as amino acids in Ringer's solution are presented.

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“…4). Furthermore, catecholamines (dopamine, epinephrine, and norepinephrine) cannot interfere since they were not detected at concentrations lower than 1610 26 mol/L, which was far above their concentration in brain microdialysate, i.e., in the 10 29 -10 210 mol/L range [34][35][36].…”

Section: Validation Of 5-ht Detectionmentioning

confidence: 98%

Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection

et al. 2005

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Serotonin or 5-hydroxytryptamine (5-HT) is a major neurotransmitter in the central nervous system. In this work, a method for analyzing 5-HT in brain microdialysis samples using a commercially available capillary electrophoresis (CE) system has been developed. A pH-mediated in-capillary preconcentration of samples was performed, and after separation by capillary zone electrophoresis, native fluorescence of 5-HT was detected by a 266 nm solid-state laser. The separation conditions for the analysis of 5-HT in standard solutions and microdialysates have been optimized, and this method has been validated on both pharmacological and analytical bases. Separation of 5-HT was performed using a 80 mmol/L citrate buffer, pH 2.5, containing 20 mmol/L hydroxypropyl-beta-cyclodextrin (HP-beta-CD) and +30 kV voltage. The detection limit was 2.5 x 10(-10) mol/L. This method allows the in vivo brain monitoring of 5-HT using a simple, accurate CE measurement in underivatized microdialysis samples.

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Cited by 55 publications

References 51 publications

Capillary electrophoresis combined with microdialysis in the human spinal cord: A new tool for monitoring rapid peroperative changes in amino acid neurotransmitters within the dorsal horn

Capillary electrophoresis combined with microdialysis in the human spinal cord: A new tool for monitoring rapid peroperative changes in amino acid neurotransmitters within the dorsal horn

HPLC-fluorescence detection and MEKC-LIF detection for the study of amino acids and catecholamines labelled with naphthalene-2,3-dicarboxyaldehyde

Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection

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