2-DE-based proteomic analysis of common bean (Phaseolus vulgaris L.) seeds

Tuber proteome comparison of five potato varieties by principal component analysis

“…There are many quantitative methods already established for plant proteomic analyses; for instance, two‐dimensional gel electrophoresis (2‐DE) is one of the most common techniques . Proteomic analysis using 2‐DE demonstrated the capacity to characterize and differentiate varieties on their protein content levels in several plants such as soybean, maize, common bean, rice and wheat, among others. The technique also provided molecular information for classification of varieties in diverse crops such as barley, maize, wheat and rice .…”

Leaf proteome comparison of two GM common bean varieties and their non‐GM counterparts by principal component analysis

“…Among other methods established in proteomics, two‐dimensional gel electrophoresis (2DE) is a simple and frequently used technique . Proteomic analysis using 2DE has demonstrated the capacity to differentiate varieties on their protein content in several plants, such as common bean, soybean, maize, wheat and rice . In the present study, we used proteomic analysis and PCA to compare four Brazilian common bean varieties ( Phaseolus vulgaris L. Embrapa 5.1 derived from the varieties Pérola and Pontal and their non‐GM counterpart varieties).…”

Protein expression profiling in haemocytes and plasma of the Manila clam Ruditapes philippinarum in response to infection with Perkinsus olseni

“…These discrepancies could correspond to isoforms generated by a variety of causes, including post‐translational modifications, alternative splicing and the occurrence of multigene families (De La Fuente et al . ). Identified proteins appearing after challenge with P. olseni zoospores were integrin‐α PS3 in haemocytes and C‐type lysozyme 2, CUB and sushi domain‐containing protein 2, Pin2‐interacting protein X1 (PinX1), lysine‐specific demethylase 3B and sarcoplasmic calcium‐binding protein (SCP) in plasma.…”

“…Remarkable discrepancies were found between the theoretical values of molecular weight and/or isoelectric points of some of the identified proteins and the observed values according to the position of the spots in the gels. These discrepancies could correspond to isoforms generated by a variety of causes, including post-translational modifications, alternative splicing and the occurrence of multigene families (De La Fuente et al 2011). Identified proteins appearing after challenge with P. olseni zoospores were integrin-a PS3 in haemocytes and C-type lysozyme 2, CUB and sushi domain-containing protein 2, Pin2-interacting protein X1 (PinX1), lysine-specific demethylase 3B and sarcoplasmic calcium-binding protein (SCP) in plasma.…”