19F MRI Probes with Tunable Switches and Highly Sensitive 19F MRI Nanoprobes

Kikuchi, Kazuya

doi:10.1246/bcsj.20140392

Cited by 11 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Understanding how cells behave at material interfaces holds wide importance for key biological applications such as cell–material surface interactions [ 1 ], mechanobiology [ 2 ], and advanced cell analysis [ 3 ]. Among such applications, one of the most practical and widely methods used across the biological sciences is cell fixation, which is an essential process for histological analyses in clinical diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Mechanical properties of paraformaldehyde-treated individual cells investigated by atomic force microscopy and scanning ion conductance microscopy

Kim

Okajima

et al. 2017

Nano Convergence

View full text Add to dashboard Cite

BackgroundCell fixation is an essential step to preserve cell samples for a wide range of biological assays involving histochemical and cytochemical analysis. Paraformaldehyde (PFA) has been widely used as a cross-linking fixation agent. It has been empirically recognized in a gold standard protocol that the PFA concentration for cell fixation, C PFA, is 4%. However, it is still not quantitatively clear how the conventional protocol of C PFA is optimized.MethodsHere, we investigated the mechanical properties of cell fixation as a function of C PFA by using atomic force microscopy and scanning ion conductance microscopy. The goal of this study is to investigate the effect of C PFA (0–10 wt%) on the morphological and mechanical properties of live and fixed mouse fibroblast cells.ResultsWe found that both Young’s modulus, E, and the fluctuation amplitude of apical cell membrane, a m, were almost constant in a lower C PFA (<10−4%). Interestingly, in an intermediate C PFA between 10−1 and 4%, E dramatically increased whereas a m abruptly decreased, indicating that entire cells begin to fix at C PFA = ca. 10−1%. Moreover, these quantities were unchanged in a higher C PFA (>4%), indicating that the cell fixation is stabilized at C PFA = ca. 4%, which is consistent with the empirical concentration of cell fixation optimized in biological protocols.ConclusionsTaken together, these findings offer a deeper understanding of how varying PFA concentrations influence the mechanical properties of cells and suggest new avenues for establishing refined cell fixation protocols.

show abstract

Section: Introductionmentioning

confidence: 99%

Mechanical properties of paraformaldehyde-treated individual cells investigated by atomic force microscopy and scanning ion conductance microscopy

Kim

Okajima

et al. 2017

Nano Convergence

View full text Add to dashboard Cite

show abstract

“…Kikuchi et al [274] developed novel multifunctional core-shell NPs utilizing the PRE effect to detect enzymatic (Caspase-3) activity and to use 19 F MRI for detecting gene expression. Paramagnetic relaxation enhancement (PRE) is the liaison between two magnetic moments of paramagnetic nuclei and observed nuclei, resulting in efficient curtailment of T 1 and T 2 of the nuclei under observation [275,276].…”

Section: Silica Npsmentioning

confidence: 99%

Nanotechnology as a Versatile Tool for 19F-MRI Agent’s Formulation: A Glimpse into the Use of Perfluorinated and Fluorinated Compounds in Nanoparticles

et al. 2022

View full text Add to dashboard Cite

Simultaneously being a non-radiative and non-invasive technique makes magnetic resonance imaging (MRI) one of the highly sought imaging techniques for the early diagnosis and treatment of diseases. Despite more than four decades of research on finding a suitable imaging agent from fluorine for clinical applications, it still lingers as a challenge to get the regulatory approval compared to its hydrogen counterpart. The pertinent hurdle is the simultaneous intrinsic hydrophobicity and lipophobicity of fluorine and its derivatives that make them insoluble in any liquids, strongly limiting their application in areas such as targeted delivery. A blossoming technique to circumvent the unfavorable physicochemical characteristics of perfluorocarbon compounds (PFCs) and guarantee a high local concentration of fluorine in the desired body part is to encapsulate them in nanosystems. In this review, we will be emphasizing different types of nanocarrier systems studied to encapsulate various PFCs and fluorinated compounds, headway to be applied as a contrast agent (CA) in fluorine-19 MRI (19F MRI). We would also scrutinize, especially from studies over the last decade, the different types of PFCs and their specific applications and limitations concerning the nanoparticle (NP) system used to encapsulate them. A critical evaluation for future opportunities would be speculated.

show abstract

“…The cleavage of the linker sets the paramagnetic metal apart from the 19 F label, thereby restoring the 19 F NMR signals. This interesting transduction mechanism was first exploited by Kikuchi and co-workers, where they designed a 19 F MR imaging probe ( 139 ) to detect protease activity. , In their design, the carboxylic acid bearing a cyclen ligand was connected to the 19 F-label through a peptide linkage, the sequence of which is specifically recognized by caspase-3. The chelated Gd 3+ is used to induce PRE effect, owing to its large spin quantum numbers. , Upon the treatment with caspase-3, the 19 F NMR signals of the probe became sharper and higher in a time-dependent manner, which suggested the PRE effect was removed through the enzymatic reaction (Figure ).…”

Section: Sensors Relying On the Modulation Of Relaxation And Chemical...mentioning

confidence: 99%

Molecular Sensors for NMR-Based Detection

Liu

Zhao

et al. 2018

Chem. Rev.

View full text Add to dashboard Cite

Reliable and precise methods capable of unambiguously identifying target analytes in real-world samples are indispensable in various fields, ranging from biological studies and diagnosis to quality control. Among various analytic techniques, nuclear magnetic resonance (NMR) is uniquely powerful as it provides multidimensional data useful for structural analysis at the atomic level. The rich information obtained from various NMR experiments allows one to access not only molecular structures and interactions but also the dynamics and diffusional properties. However, the interpretation of NMR data in the analysis of real-world mixtures can be challenging and is often complicated by the overlap of the NMR resonances of each component. Moreover, the inherently low sensitivity of the NMR technique hampers its implementation in many detections, where the analytes of interest are present at low concentrations. By a combination of heteronuclear NMR, dedicatedly designed sensors, ingenious transduction mechanisms, and powerful NMR pulse sequences, significant advancements were made to conquer these limitations. The present review summarizes the sensing systems that effectively facilitate NMR-based detection with an emphasis on the chemical perspective of sensor design and transduction mechanism. Advances in hyperpolarized sensors to boost the sensitivity of detection will also be included where appropriate.

show abstract

19F MRI Probes with Tunable Switches and Highly Sensitive 19F MRI Nanoprobes

Cited by 11 publications

References 14 publications

Mechanical properties of paraformaldehyde-treated individual cells investigated by atomic force microscopy and scanning ion conductance microscopy

Mechanical properties of paraformaldehyde-treated individual cells investigated by atomic force microscopy and scanning ion conductance microscopy

Nanotechnology as a Versatile Tool for 19F-MRI Agent’s Formulation: A Glimpse into the Use of Perfluorinated and Fluorinated Compounds in Nanoparticles

Molecular Sensors for NMR-Based Detection

Contact Info

Product

Resources

About