1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells

Abstract

More studies that are focused on the bioeffects of radio-frequency (RF) electromagnetic radiation that is generated from the communication devices, but there were few reports with confirmed results about the bioeffects of RF radiation on reproductive cells. To explore the effects of 1950 MHz RF electromagnetic radiation (EMR) on mouse Leydig (TM3) cells. TM3 cells were irradiated or sham-irradiated continuously for 24 h by the specific absorption rate (SAR) 3 W/kg radiation. At 0, 1, 2, 3, 4, and 5 days after irradiation, cell proliferation was detected by cell counting kit-8 (CCK-8) method, cell cycle distribution, percentage of apoptosis, and cellular reactive oxygen species (ROS) were examined by flow cytometry, Testosterone level was measured using enzyme-linked immunosorbent assay (ELISA) assay, messenger ribonucleic acid (mRNA) expression level of steroidogenic acute regulatory protein (StAR) and P450scc in TM3 cells was detected by real-time polymerase chain reaction (PCR). After being irradiated for 24 h, cell proliferation obviously decreased and cell cycle distribution, secretion capacity of Testosterone, and P450scc mRNA level were reduced. While cell apoptosis, ROS, and StAR mRNA level did not change significantly. The current results indicated that 24 h of exposure at 1950 MHz 3 W/kg radiation could cause some adverse effects on TM3 cells proliferation and Testosterone secretion, further studies about the biological effects in the reproductive system that are induced by RF radiation are also needed.

Apoptotic Effect of 1800 MHz Electromagnetic Radiation on NIH/3T3 Cells

“…Meanwhile, CCK-8 results showed that cell viability significantly declined after 12, 36, and 48 h EMR exposure. Similar to our findings, Liu et al found that 1950 MHz EMR caused the percentage of apoptotic cells to significantly increase after 48 h [25]; our previous research results showed that 1800 MHz EMR for 1, 4, and 8 h caused a significant increase in the percentage of late apoptotic cells [24]; Lin et al found that 1950 MHz, SAR 3 W/kg EMR for 24 h can cause adverse effects on TM3 cell proliferation [26]; Bilal et al indicated that mobile phone radiation had an obvious apoptotic effect on MCF-7 cells [27]; Kesari et al claimed that 3G cell phone network exposure caused cell apoptosis in rat brain [28]; and Buttiglione et al proved that cell viability significantly decreased after 24 h of RF-EMF exposure [29]. In order to qualitatively study the cellular biological effects of cell phone radiation, this study simulated cell phone radiation at 1800 MHz, 2 W/kg.…”

Alteration of testicular regulatory and functional molecules following long‐time exposure to 900 MHz RFW emitted from BTS

“…Exposure of Leydig cells (in vitro) to 1,950 MHz RF was also reported to decrease P450scc expression level without changes in StAR (Lin et al, ). However, high‐dose exposure to cell phone radiation was reported to reduce expression of StAR and testosterone concentration in the testes of mice (Zang et al, ).…”

The protective effect of melatonin on radiofrequency electromagnetic fields of mobile phone‐induced testicular damage in an experimental mouse model

“…Many studies show that EMR exposure can affect on the quality of semen and reduce sperm count, change sperm motility and histological structure of seminiferous tubule, finally leading to infertility in human and animal model (Al‐Bayyari, 2017; Gautam et al., 2019). Also, EMR reduces blood testosterone through oxidative stress and its effect on Leydig cells (Lin et al., 2018).…”