14-3-3 targets keratin intermediate filaments to mechanically sensitive cell–cell contacts

Mariani, Richard A.; Paranjpe, Shalaka; Dobrowolski, Radoslaw; Weber, Gregory F.

doi:10.1091/mbc.e18-06-0373

Cited by 11 publications

(9 citation statements)

References 66 publications

(125 reference statements)

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“… (2) Evolve new proteins and structures that are absent in nonvertebrates. Many newly emerged vertebrate proteins and structures are designed to work with conserved molecules and core mechanisms to increase complexity ( Mariani et al. , 2020 ).…”

Section: Tuning Force In a Stiffer Environment Supported By Whole Genmentioning

confidence: 99%

Collagen, stiffness, and adhesion: the evolutionary basis of vertebrate mechanobiology

Tang

2020

MBoC

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The emergence of collagen I in vertebrates resulted in a dramatic increase in the stiffness of the extracellular environment, supporting long-range force propagation and the development of low-compliant tissues necessary for the development of vertebrate traits including pressurized circulation and renal filtration. Vertebrates have also evolved integrins that can bind to collagens, resulting in the generation of higher tension and more efficient force transmission in the extracellular matrix. The stiffer environment provides an opportunity for the vertebrates to create new structures such as the stress fibers, new cell types such as endothelial cells, new developmental processes such as neural crest delamination, and new tissue organizations such as the blood–brain barrier. Molecular players found only in vertebrates allow the modification of conserved mechanisms as well as the design of novel strategies that can better serve the physiological needs of the vertebrates. These innovations collectively contribute to novel morphogenetic behaviors and unprecedented increases in the complexities of tissue mechanics and functions.

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Section: Tuning Force In a Stiffer Environment Supported By Whole Genmentioning

confidence: 99%

Collagen, stiffness, and adhesion: the evolutionary basis of vertebrate mechanobiology

Tang

2020

MBoC

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“…Changes in local microenvironment, particularly force distribution, may affect keratin-mediated signaling pathways through changing keratin network conformation and/or properties, following posttranslational modifications (Snider and Omary, 2014;Loschke et al, 2015). Moreover, keratins act by scaffolding signaling proteins such as Src, PKC, TRADD, 14-3-3, and Fas receptors (Gilbert et al, 2001;Inada et al, 2001;Kröger et al, 2013;Rotty and Coulombe, 2012;Sanghvi-Shah and Weber, 2017;Mariani et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Disease-associated keratin mutations reduce traction forces and compromise adhesion and collective migration

Fujiwara

Deguchi

Magin

2020

Journal of Cell Science

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Keratin intermediate filament (IF) proteins constitute the major cytoskeletal components in epithelial cells. Missense mutations in keratin 5 (K5; also known as KRT5) or keratin 14 (K14; also known as KRT14), highly expressed in the basal epidermis, cause the severe skin blistering disease epidermolysis bullosa simplex (EBS). EBS-associated mutations disrupt keratin networks and change keratinocyte mechanics; however, molecular mechanisms by which mutations shape EBS pathology remain incompletely understood. Here, we demonstrate that, in contrast to keratin-deficient keratinocytes, cells expressing K14R125C, a mutation that causes severe EBS, generate lower traction forces, accompanied by immature focal adhesions with an altered cellular distribution. Furthermore, mutant keratinocytes display reduced directionality during collective migration. Notably, RhoA activity is downregulated in human EBS keratinocytes, and Rho activation rescues stiffness-dependent cell–extracellular matrix (ECM) adhesion formation of EBS keratinocytes. Collectively, our results strongly suggest that intact keratin IF networks regulate mechanotransduction through a Rho signaling pathway upstream of cell–ECM adhesion formation and organized cell migration. Our findings provide insights into the underlying pathophysiology of EBS.This article has an associated First Person interview with the first author of the paper.

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“…The intermediate cell layer was mainly manifested by remarkable bundles of the cytokeratin filaments only in the investigated ducks. In our theory, these filaments were essential to provide a mechanical resistance and withstand any strains, which resulted from the large food boluses swallowed by domesticated ducks [39] and desmosomes at the levels of their cellular interdigitation in all studied species [40]. The superficial layer of a unique criterion in the pigeon is the most outer surface cells showing fine cellular processes rather than the desmosomes that are characteristics to the remaining superficial cells.…”

Section: Discussionmentioning

confidence: 99%

Crop Morpho-Histological Peculiarities in Domesticated Pigeons (Columba livia domestica), Cattle Egret (Bubulcus ibis) and Domesticated Ducks (Anas platyrhynchos domestica)

Mohammed

Abuel-Atta

Ghonimi

et al. 2021

Zagazig Veterinary Journal

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The crop architecture varies among different avian species consistent with their feeding habits. Therefore, twenty-one of mature healthy male pigeons (granivorous), cattle egrets (carnivorous), and ducks (omnivorous), seven per species, were utilized for the histological, immunohistochemical, and ultrastructural analyses. Histologically, the mucosal folds were covered by non-keratinized stratified squamous epithelium in the three investigated species. The mucosal glands are only peculiar to the crops of the cattle egrets as well as the ducks, while the pigeons' crops were devoid of any secretory units. Morphometrically, the optical densities of the Alcian blue (AB) and periodic acid Schiff (PAS) reactions in the secretory glands, the area % of collagen fibers, the thickness of the tunica musculosa were measured and declared significant differences among the three studied avian species. Immunohistochemical reaction revealed ki-67 immuno-positive reactivity in the nuclei of basal cell layers of the crop epithelium in pigeons only. Regarding ultrastructure investigation, the covering epithelium of all studied species had been shown to be the basal layer of cuboidal to tall columnar cells with desmosomal junctions at the level of their cellular interdigitation, intermediate layers of large irregular polygonal cells with obviously increased cytokeratin filaments especially in the ducks and cellular interdigitation in between. The superficial layer of flat-shaped cells in the three investigated species manifested by desmosomal junctions in between but at the level of the surface squamous cells of the superficial layer, a fine lateral process is only inspected in the pigeons. In conclusion, the crop glands characterized by supranuclear electron dense secretory granules in the cattle egrets and electron-lucent in ducks.

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14-3-3 targets keratin intermediate filaments to mechanically sensitive cell–cell contacts

Cited by 11 publications

References 66 publications

Collagen, stiffness, and adhesion: the evolutionary basis of vertebrate mechanobiology

Collagen, stiffness, and adhesion: the evolutionary basis of vertebrate mechanobiology

Disease-associated keratin mutations reduce traction forces and compromise adhesion and collective migration

Crop Morpho-Histological Peculiarities in Domesticated Pigeons (Columba livia domestica), Cattle Egret (Bubulcus ibis) and Domesticated Ducks (Anas platyrhynchos domestica)

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