13C nuclear magnetic resonance spectroscopy for determining the different components of epicuticular waxes of olive fruit (Olea europaea) Dritta cultivar

Vlahov, Giovanna; Rinaldi, Giuseppe; Re, Paolo Del; Giuliani, Angela

doi:10.1016/j.aca.2008.06.049

Cited by 23 publications

(16 citation statements)

References 20 publications

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“…More than 30 elemental formulae were attributable to wax esters from C31 to C44. Among them, benzyl esters of FAs from C22 to C30 were detected, in agreement with Bianchi et al, with C26 and C28 being the most abundant, in agreement with Biedermann et al Moreover, diterpene esters such as geranylgeranyl and phytyl esters were detected for FAs from C16 to C22. Finally, formulae matching with aliphatic waxes from C34 to C44 were found .…”

Section: Resultssupporting

confidence: 88%

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Direct chemical profiling of olive (Olea europaea) fruit epicuticular waxes by direct electrospray-ultrahigh resolution mass spectrometry

Vichi¹,

Cortés-Francisco²,

Romero

et al. 2015

J. Mass Spectrom.

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In the present paper, an electrospray ionization (ESI)-Orbitrap method is proposed for the direct chemical profiling of epicuticular wax (EW) from Olea europaea fruit. It constitutes a rapid and efficient tool suitable for a wide-ranging screening of a large number of samples. In a few minutes, the method provides a comprehensive characterization of total EW extracts, based on the molecular formula of their components. Accurate mass measurements are obtained by ultrahigh resolution mass spectrometry, and compositional restrictions are set on the basis of the information available from previous studies of olive EW. By alternating positive and negative ESI modes within the same analysis, complementary results are obtained and a wide range of chemical species is covered. This provides a detailed compositional overview that otherwise would only be available by applying multiple analytical techniques.

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Section: Resultssupporting

confidence: 88%

“…Genetic and metabolite profiling has been carried out on a number of plant species, but very few studies have characterized olive fruit EW. Those studies evidenced the presence of alkanes, alcohols, aldehydes, alkyl and phenyl esters, triacylglycerols (TAGs), triterpenols, FAs and triterpenic acids, among others …”

Section: Introductionmentioning

confidence: 99%

Direct chemical profiling of olive (Olea europaea) fruit epicuticular waxes by direct electrospray-ultrahigh resolution mass spectrometry

Vichi¹,

Cortés-Francisco²,

Romero

et al. 2015

J. Mass Spectrom.

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show abstract

“…MMH yielded five fractions 15-19, and MMA four fractions 20-23 Fraction 15 (12.2 g) eluted with cyclohexane/EtOAc (70:30) afforded a mixture of unsaturated linear fatty acid 5 (130 mg) (Mossoba et al, 1990;Vlahov et al, 2008) (Table 2, Fig. 1).…”

Section: Plant Materialsmentioning

confidence: 99%

“…1). Fraction 20 (11 g) was eluted with cyclohexane/EtOAc (80:20) to afford a mixture of linear diunsaturated fatty acid ethyl esters 7 (110 mg) (Mossoba et al, 1990;Vlahov et al, 2008) (Table 2, Fig. 1).…”

Section: Plant Materialsmentioning

confidence: 99%

In vitro antimicrobial activity of extracts and compounds of some selected medicinal plants from Cameroon

Mbosso

Ngouela

Nguedia

et al. 2010

Journal of Ethnopharmacology

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“…These studies suggested a possibility for the new use of NMDA receptor blockers in the treatment of stroke. Maslinic acid (2-α,3-β-dihydroxyolean-12-en-28-oic acid) is an oleanane-type triterpenoid compound abundantly expressed in the wax skin of Olea europaea, a species of small tree in the family Oleaceae ( Figure 1A) [11]. It was previously demonstrated as a potent inhibitor of astrocytic glycogen phosphorylase with low toxicity [12,13], and has been applied in pathological processes including hypoglycemia and diabetes [14,15] due to its glucose-lowering effects by interfering with abnormal glycogen metabolism.…”

Section: Introductionmentioning

confidence: 99%

Neuroprotection by Combined Administration with Maslinic Acid, a Natural Product from Olea europaea, and MK-801 in the Cerebral Ischemia Model

et al. 2016

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Glutamate-mediated excitotoxicity is a major cause of ischemic brain damage. MK-801 confers neuroprotection by attenuating the activation of the N-methyl-D-aspartate (NMDA) receptor, but it failed in clinical use due to the short therapeutic window. Here we aim to investigate the effects of maslinic acid, a natural product from Olea europaea, on the therapeutic time window and dose range for the neuroprotection of MK-801. Rats were administered with maslinic acid intracerebroventricularly and cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) followed by reperfusion. MK-801 was administered at 1 h, 2 h, 3 h and 4 h after ischemia, respectively. The cerebral infarct volume was determined by 2,3,5-Triphenyltetrazolium chloride (TTC) staining, neuronal damage was assessed by Haematoxylin Eosin (H&E) staining, and the expression of glial glutamate transporters and glial fibrillary acidic protein (GFAP) was evaluated by immunohistochemistry and Western blot post-ischemia. Results showed that the presence of maslinic acid extended the therapeutic time window for MK-801 from 1 h to 3 h. Co-treatment of maslinic acid and MK-801 at a subthreshold dosage obviously induced neuroprotection after ischemia. The combination of these two compounds improved the outcome in ischemic rats. Moreover, maslinic acid treatment promoted the expression of GLT-1 and GFAP post-ischemia. These data suggest that the synergistic effect of maslinic acid on neurological protection might be associated with the improvement of glial function, especially with the increased expression of GLT-1. The combination therapy of maslinic acid and MK-801 may prove to be a potential strategy for treating acute ischemic stroke.

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13C nuclear magnetic resonance spectroscopy for determining the different components of epicuticular waxes of olive fruit (Olea europaea) Dritta cultivar

Cited by 23 publications

References 20 publications

Direct chemical profiling of olive (Olea europaea) fruit epicuticular waxes by direct electrospray-ultrahigh resolution mass spectrometry

Direct chemical profiling of olive (Olea europaea) fruit epicuticular waxes by direct electrospray-ultrahigh resolution mass spectrometry

In vitro antimicrobial activity of extracts and compounds of some selected medicinal plants from Cameroon

Neuroprotection by Combined Administration with Maslinic Acid, a Natural Product from Olea europaea, and MK-801 in the Cerebral Ischemia Model

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