11β-Hydroxysteroid dehydrogenase in the rat ovary: high expression in the oocyte

Abstract

The enzyme 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) catalyses the conversion of physiological glucocorticoids to inactive products, thus modifying the access of glucocorticoids to glucocorticoid and mineralocorticoid receptors. Glucocorticoids may affect ovarian function both indirectly and via binding to ovarian receptors. We have demonstrated 11 beta-HSD bioactivity and mRNA expression in rat ovary in vitro. The enzyme was localized to oocytes and luteal bodies immunohistochemically using two antibodies raised against purified rat liver 11 beta-HSD. These data are supported by in-situ hybridization studies, which also localized 11 beta-HSD mRNA expression to oocytes and luteal bodies. The results suggest that 11 beta-HSD may modulate the effects of glucocorticoid on ovarian function.

Direct inhibition of ovarian steroidogenesis by Cortisol and the modulatory role of 11β‐hydroxysteroid dehydrogenase

“…In many tissues, the actions of cortisol are modulated by a microsomal enzyme, 1 lp-hydroxysteroid dehydrogenase (1 1 PHSD) which metabolizes cortisol to its inactive derivative, cortisone. Previous studies have demonstrated this conversion in homogenized ovaries (Murphy, 198 1 ;Benediktsson et al, 1992), and have identified the messenger RNA encoding llPHSD in ovarian tissue (Tannin et al, 1991;Whorwood et al, 1992;Benediktsson et al, 1992). In this study, we have investigated the effects of cortisol on LHstimulated steroidogenesis in cultured human granulosalutein cells and have established a role for llPHSD in regulating the sensitivity of these cells to cortisol.…”

Dual Function of 11β-Hydroxysteroid Dehydrogenase in Placenta: Modulating Placental Glucocorticoid Passage and Local Steroid Action1

“…Accordingly, 11␤-HSD-1 messenger RNA expression is highly correlated with 11-oxoreductase activity in rat myometrium [22] and placenta (see below). The 11␤-HSD-1 enzyme is widely distributed in glucocorticoid target tissues including the liver [23], brain [24,25], uterus [22,[26][27][28], and ovary [29].…”

Do platelet rich plasma and folic acid reverse experimental induced ovarian failure: Emphasis on folliculogenesis, and ovarian steroidogenesis

“…The protein encoded by 11β-HSD gene is 11β-HSD enzyme that catalyzes the transformation of inert 11 keto-products (cortisone) to active cortisol, consequently, modulating the access of glucocorticoids to steroid receptors. Notably, glucocorticoids may affect ovarian function both indirectly and directly via binding to ovarian receptors [49,50] . HSD3B2 gene is primarily expressed in the adrenal glands and the gonads.…”