1148 Comparative MicroRNA Expression Profiles in Human Cervical Cancer

Gőcze, Katalin; Kovács, Krisztina; Kajtár, B.; Benczik, Márta; Göcze, Péter M.; Ember, István

doi:10.1016/s0959-8049(12)71744-6

Cited by 43 publications

(61 citation statements)

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“…Multivariate analysis revealed that serum miR-196a was an independent risk factor for cervical cancer, indicating that serum miR-196a may be a promising prognostic biomarker. Similar to our results, previous studies have found that miR-196a is overexpressed in cervical cancer tissues, CIN tissues, and cervical cancer cell lines (Gocze et al, 2013;Zhang et al, 2013;VillegasRuiz et al, 2014;Hou et al, 2014). Specifically, Hou et al (2014) reported that upregulation of tissue miR-196a in patients with cervical cancer was associated with poor clinical outcome, indicating that miR-196a may function as an oncogene in cervical cancer.…”

Section: Discussionsupporting

confidence: 91%

Clinical significance of serum miR-196a in cervical intraepithelial neoplasia and cervical cancer

Liu¹,

Xin²,

F³

2015

Genet. Mol. Res.

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ABSTRACT. Previous studies have reported that miR-196a is upregulated in cervical cancer tissues and cell lines. However, whether serum miR196a is increased in patients with cervical cancer or cervical intraepithelial neoplasia (CIN), and its potential clinical value remained unknown. In total, 105 cervical cancer patients, 86 CIN patients, and 50 healthy volunteers were recruited. Quantitative reverse transcription-polymerase chain reaction was performed to compare the serum levels miR-196a in all participants. The associations between serum miR-196a and CIN grade/ clinicopathological parameters of cervical cancer were also examined. A survival analysis was performed using the Kaplan-Meier method. Univariate and multivariate analyses were conducted to explore the independent risk factors for cervical cancer. Our results revealed that serum miR196a levels were higher in patients with cervical cancer (P < 0.01) and CIN (P < 0.05) compared to those in healthy controls. Serum miR-196a was associated with CIN grade and various cervical cancer parameters including tumor size (P = 0.031), lymph node metastasis (P = 0.018),

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Section: Discussionsupporting

confidence: 91%

Clinical significance of serum miR-196a in cervical intraepithelial neoplasia and cervical cancer

Liu¹,

Xin²,

F³

2015

Genet. Mol. Res.

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“…The funding genes or oncogenic genes in cancer tumorigenesis as well as in gastric cancer (Shenouda and Alahari, 2009;Bartels and Tsongalis, 2010;Cortes-Sempere and Ibanez de Caceres, 2011). Mir-196a is a newly reported miRNA biomarkers relevant to multiple cancers, such as breast cancer (Lee et al, 2014), lung cancer , cervical cancer (Gocze et al, 2013), renal cancer (Du et al, 2014a), head and neck cancer (Christensen et al, 2010), hepatocellular cancer (Hao et al, 2013), pancreatic cancer (Slater et al, 2014), colorectal cancer (Zhan et al, 2011;Du et al, 2014b) and early gastric cancer (Zheng et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Clinical Significance of Upregulation of mir-196a-5p in Gastric Cancer and Enriched KEGG Pathway Analysis of Target Genes

Xie²,

Yang³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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“…However, Gocze et al (29) used PCR to detect the expression of 8 different miRs in cervical cancer tissues, and observed that the expression levels of miR-203 were significantly higher in cervical cancer tissue samples, compared with normal tissues. Therefore, the expression of miR-203 in cervical cancer remains under debate.…”

Section: Discussionmentioning

confidence: 99%

Low folate levels are associated with methylation-mediated transcriptional repression of miR-203 and miR-375 during cervical carcinogenesis

et al. 2016

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Abstract. The aim of the present study was to investigate the correlation between a lack of folic acid and the abnormal expression of microRNA (miR)-203 and miR-375 in cervical cancer. In total, 60 tissue samples of cervical intraepithelial neoplasia (CIN) or stage IA-IIA cervical cancer (study group), and 30 samples without soluble interleukin or malignancy (control group) were examined. The expression of miR-203 and miR-375 was detected using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and the difference in expression levels was quantified using the 2 -ΔΔCq method. In addition, CaSki cervical cancer cells were cultured in vitro and treated with various concentrations of folic acid. The DNA methylation states of miR-203 and miR-375 were subsequently detected by methylation-specific PCR, and the expression levels were evaluated using RT-PCR. miR-203 and miR-375 were significantly downregulated in CIN and cervical cancer tissues, compared with the control group. There was a marked difference in terms of the expression levels of miR-375 between the two groups (P<0.05). In CaSki cells, as the concentration of folic acid increased, the positive rate of DNA methylation of miR-203 and miR-375 decreased, while the expression levels of miR-203 and miR-375 demonstrated a gradual increase, which indicated that the latter two parameters were negatively correlated (P<0.05). Compared with normal cervical tissue, the expression levels of miR-203 and miR-375 were downregulated in CIN and cervical cancer. Methylation of these two miRs was apparent in CaSki cells, and was associated with a lack of folic acid. Therefore, reduced levels of folic acid, leading to increased methylation of miR-203 and miR-375, may be significant events during cervical carcinogenesis.

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1148 Comparative MicroRNA Expression Profiles in Human Cervical Cancer

Cited by 43 publications

References 0 publications

Clinical significance of serum miR-196a in cervical intraepithelial neoplasia and cervical cancer

Clinical significance of serum miR-196a in cervical intraepithelial neoplasia and cervical cancer

Clinical Significance of Upregulation of mir-196a-5p in Gastric Cancer and Enriched KEGG Pathway Analysis of Target Genes

Low folate levels are associated with methylation-mediated transcriptional repression of miR-203 and miR-375 during cervical carcinogenesis

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