Isolation, Purification and Partial Characterization of Urokinase From Cow Urine of Indian Origin (Bos Indicus)

Abstract

In Ayurveda, there are many medicines made from cow urine (Goumutra). This purifies, and clears all blocks in body channels (shroto-shodhaka). It enhances the therapeutic actions of medicines taken along with it. Cow urine has a unique place in Ayurveda and has been described in many ancient manuscripts like Atharva Veda, Charaka Samhita, Rajni Ghuntu, Vridhabhagabhatt, Amritasagar, Bhavaprakash, Sachitra Ayurveda, and Sushruta Samhita to be the most effective substance / secretion of animal origin with therapeutic values. It has been recognized as 'Water of Life' or 'Amrita'. In India, drinking of cow urine has been practiced for thousands of years. Cow Urine Treatment And Research Center, Indore, India and Go Vigyan Anusandhan Kendra, Nagpur, India has conducted a lot of research over the past few years and has reached the conclusion that it is capable of curing thrombo embolic diseases, diabetes, blood pressure, asthma, psoriasis, eczema, heart attack, blockage in arteries, fits, cancer, piles, prostrate, arthritis, migraine, thyroid, ulcer, acidity, constipation, gynecological problems, ear and nose problems and several other diseases. The present work focuses on the isolation, concentration, purification of urokinase from freshly collected samples of cow urine from cows of Indian origin (Bos indicus), its partial characterization by SDS-PAGE, TLC( Tryptic digest protein) and proteolytic activity by gelatin zymography. The dialyzed cow urine proteins showed band identical to standard urokinase by SDS-PAGE. The itching of gelatin by dialyzed cow urine also shows the proteolytic activity.