Long–term lung inflammation is reduced by estradiol treatment in brain dead female rats

Ricardo‐da‐Silva, Fernanda Yamamoto; Armstrong‐Jr, Roberto; Vidal‐dos‐Santos, Marina; Correia, Cristiano de Jesus; Silva, Raphael dos Santos Coutinho e; Anunciação, Lucas Ferreira da; Moreira, Luíz Felipe Pinho; Leuvenink, Henri G. D.; Breithaupt‐Faloppa, Ana Cristina

doi:10.6061/clinics/2021/e3042

Cited by 4 publications

(2 citation statements)

References 30 publications

(41 reference statements)

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“…storm leads to the disruption of endothelial cells and the alveolar barrier (23), and, acute systemic inflammation leads to the infiltration of activated neutrophils to the lungs, leading to tissue injury (28,29). Previous studies from our group have shown that BD increases inflammatory markers in lung tissue in the short and long-term and that E2 presents anti-inflammatory properties that could attenuate lung injury (13,14). The same behavior can be observed in this study after 6h of BD by the increased levels of…”

Section: A B Figure 12mentioning

confidence: 99%

“…In experimental models, BD in females is followed by the acute reduction of estradiol (E2) and corticosterone with higher inflammation (8). Treatment of donors with either estradiol or corticoids alone has shown positive effects in experimental and clinical studies of BD (9)(10)(11)(12)(13)(14)(15). However, in females, adequate stress response appears to be linked to the presence of both estradiol and corticoids.…”

Section: Introductionmentioning

confidence: 99%

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17β-estradiol and methylprednisolone association as a therapeutic option to modulate lung inflammation in brain-dead female rats

Vidal-dos-Santos,

Anunciação,

Armstrong-Jr

et al. 2024

Front. Immunol.

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IntroductionBrain death (BD) is known to compromise graft quality by causing hemodynamic, metabolic, and hormonal changes. The abrupt reduction of female sex hormones after BD was associated with increased lung inflammation. The use of both corticoids and estradiol independently has presented positive results in modulating BD-induced inflammatory response. However, studies have shown that for females the presence of both estrogen and corticoids is necessary to ensure adequate immune response. In that sense, this study aims to investigate how the association of methylprednisolone (MP) and estradiol (E2) could modulate the lung inflammation triggered by BD in female rats.MethodsFemale Wistar rats (8 weeks) were divided into four groups: sham (animals submitted to the surgical process, without induction of BD), BD (animals submitted to BD), MP/E2 (animals submitted to BD that received MP and E2 treatment 3h after BD induction) and MP (animals submitted to BD that received MP treatment 3h after BD induction).ResultsHemodynamics, systemic and local quantification of IL-6, IL-1β, VEGF, and TNF-α, leukocyte infiltration to the lung parenchyma and airways, and adhesion molecule expression were analyzed. After treatment, MP/E2 association was able to reinstate mean arterial pressure to levels close to Sham animals (p<0.05). BD increased leukocyte infiltration to the airways and MP/E2 was able to reduce the number of cells (p=0.0139). Also, the associated treatment modulated the vasculature by reducing the expression of VEGF (p=0.0616) and maintaining eNOS levels (p=0.004) in lung tissue.DiscussionData presented in this study show that the association between corticoids and estradiol could represent a better treatment strategy for lung inflammation in the female BD donor by presenting a positive effect in the hemodynamic management of the donor, as well as by reducing infiltrated leukocyte to the airways and release of inflammatory markers in the short and long term.

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Section: A B Figure 12mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

17β-estradiol and methylprednisolone association as a therapeutic option to modulate lung inflammation in brain-dead female rats

Vidal-dos-Santos,

Anunciação,

Armstrong-Jr

et al. 2024

Front. Immunol.

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Adipokine secretion and lipolysis following gender-affirming treatment in transgender individuals

Subramanian,

Wiik,

Rullman

et al. 2024

J Endocrinol Invest

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Background The organ-specific effects of gender-affirming sex hormone treatment (GAHT) in transgender women (TW) and transgender men (TM) are insufficiently explored. This study investigated the effects of GAHT on adipose tissue function. Methods In a single-center interventional prospective study, 32 adults undergoing GAHT, 15 TW and 17 TM, were examined with anthropometry and abdominal subcutaneous adipose tissue biopsies obtained before initiation of treatment, 1 month after endogenous sex hormone inhibition and three and 11 months after initiated GAHT. Fat cell size, basal/stimulated lipolysis and cytokine secretion in adipose tissue were analyzed. Results TW displayed an increase in complement component 3a and retinol-binding protein 4 (RBP4) secretion after sex hormone inhibition, which returned to baseline following estradiol treatment. No changes in lipolysis were seen in TW. TM showed downregulation of RBP4 after treatment, but no changes in basal lipolysis. In TM, the estrogen suppression led to higher noradrenaline stimulated (NA) lipolysis that was normalized following testosterone treatment. At 11 months, the ratio of NA/basal lipolysis was lower compared to baseline. There were no significant changes in fat cell size in either TW or TM. Conclusion In TW, gonadal hormone suppression results in transient changes in cytokines and in TM there are some changes in NA-stimulated lipolysis following testosterone treatment. However, despite the known metabolic effects of sex hormones, the overall effects of GAHT on adipose tissue function are small and likely have limited clinical relevance, but larger studies with longer follow-up are needed to confirm these findings. Trial registration ClinicalTrials.gov Identifier: NCT02518009, Retrospectively registered 7 August 2015.

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Estradiol is a key candidate for treating Ankylosing Spondylolisthesis with Traditional Chinese Medicine

Zheng

Wang

Guo

et al. 2023

Computers in Biology and Medicine

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Long–term lung inflammation is reduced by estradiol treatment in brain dead female rats

Abstract: LF, et al. Long-term lung inflammation is reduced by estradiol treatment in brain dead female rats. Clinics (Sao Paulo).

Cited by 4 publications

References 30 publications

17β-estradiol and methylprednisolone association as a therapeutic option to modulate lung inflammation in brain-dead female rats

17β-estradiol and methylprednisolone association as a therapeutic option to modulate lung inflammation in brain-dead female rats

Adipokine secretion and lipolysis following gender-affirming treatment in transgender individuals

Estradiol is a key candidate for treating Ankylosing Spondylolisthesis with Traditional Chinese Medicine

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