Changes in the intestinal microbiota of superobese patients after bariatric surgery

Abstract

OBJECTIVES:The gut microbiota is associated with obesity and weight loss after bariatric surgery and has been related to its changing pattern. Exactly how the bacterial population affects weight loss and the results of surgery remain controversial. This study aimed to evaluate the intestinal microbiota of superobese patients before and after gastric bypass surgery (RYGB).METHOD:DNA fragments for the microbiota obtained from stool samples collected from nine superobese patients before and after bariatric surgery were sequenced using Ion Torrent.RESULTS:We observed that with a mean follow-up of 15 months, patients achieved 55.9% excess weight loss (EWL). A significant population reduction in the Proteobacteria phylum (11 to 2%, p=0.0025) was observed after surgery, while no difference was seen in Firmicutes and Bacteroidetes. Further analyses performed with two specific individuals with divergent clinical outcomes showed a change in the pattern between them, with a significant increase in Firmicutes and a decrease in Bacteroidetes in the patient with less weight loss (%EWL 50.79 vs. 61.85).CONCLUSIONS:RYGB affects the microbiota of superobese patients, with a significant reduction in Proteobacteria in patients with different weight loss, showing that different bacteria may contribute to the process.

Mining Gut Microbiota From Bariatric Surgery for MAFLD

“…At the functional level, the module of propionate production was higher after RYGB which is consistent with previous animal study conducted by Liou et al (31). Other recent studies comparing gut microbiome after bariatric surgery by using 16S rRNA amplicon sequencing showed a decreased Firmicutes/Bacteroidetes ratio (40,41), indicating a favorable phylum composition for obesity which is consistent with a recent meta-analysis showing increase in phyla Bacteroidetes, Fusobacteria, Proteobacteria and Verrucomicrobia, and a decrease in Firmicutes (28).…”

Gut Microbiota, Probiotics and Psychological States and Behaviors after Bariatric Surgery—A Systematic Review of Their Interrelation

“…Out of the 59 studies, 36 studies applied a pre-post design (baseline measurements provided) (Group 1: n = 23 [ 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ]; Group 2: n = 10 [ 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 ]; Group 3: n = 4 [ 70 , 71 , 72 , 73 ]). Sixteen studies used a cross-sectional design (Group 1: n = 6 [ 74 , 75 , 76 , 77 , 78 , 79 ]; Group 2: n = 10 [ 80 , 81 , 82 , 83 , 84 , 85 , 86 ,…”

“…Changes in GI microbiota was the primary outcome for 24 studies in Group 1 [ 37 , 38 , 39 , 40 , 41 , 42 , 44 , 45 , 47 , 48 , 49 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 59 , 75 , 76 , 77 , 78 , 79 , 94 ] and 12 studies in Group 2 [ 61 , 62 , 64 , 65 , 68 , 82 , 85 , 86 , 88 , 90 , 92 , 95 ]. For the remaining studies, primary outcomes surrounding clinical, anthropometric and metabolic parameters were reported (Group 1: n = 5 [ 43 , 46 , 50 , 58 , 74 ]; Group 2: n = 13 [ 60 , 63 , 66 , 67 , 69 , 80 , 81 , 83 , …”

The Genus Parabacteroides Is a Potential Contributor to the Beneficial Effects of Truncal Vagotomy–Related Bariatric Surgery

“…Some studies have reported that the composition of gut microbiota of obese subjects present a higher Firmicutes/Bacteroidetes ratio (F/B) [31][32][33]. Whereas, with the rapid accumulation of data, several studies did not find any differences of this parameter between obese and normal-weight individuals or even reported decreased F/B ratio in obese subjects [34,35]. Furthermore, several meta-analyses have clarified that the heterogeneity of the results could be due to the insufficient number of subjects including in most of the studies [36,37].…”