2019
DOI: 10.6061/clinics/2019/e1132
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Abstract: OBJECTIVE: To describe and analyze a new protocol for the extraction of platelet-rich plasma (PRP) for use in clinical practice and compare this technique with methods that have been previously described in the medical literature. METHODS: We extracted PRP from 20 volunteers using four different protocols (single spin at 1600 ×g, single spin at 600 ×g, double spin at 300 and 700 ×g, and double spin at 600 and 900 ×g). In another group of 12 individuals, we extracted PRP… Show more

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Cited by 20 publications
(25 citation statements)
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“…The second centrifugation is performed at a higher speed, just after the end of the first one. In the present study, we upgraded the turn down-turn up technique described in Machado et al [ 14 ], facilitating its reproducibility and reducing preparation time. This two-spin method follows the principles of low-speed centrifugation to preserve the maximum volume of platelets, leukocytes, and growth factors and uses discontinuous centrifugation to modulate and control platelet recovery [ 25 ].…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The second centrifugation is performed at a higher speed, just after the end of the first one. In the present study, we upgraded the turn down-turn up technique described in Machado et al [ 14 ], facilitating its reproducibility and reducing preparation time. This two-spin method follows the principles of low-speed centrifugation to preserve the maximum volume of platelets, leukocytes, and growth factors and uses discontinuous centrifugation to modulate and control platelet recovery [ 25 ].…”
Section: Discussionmentioning
confidence: 99%
“…The lack of standardization and the high cost of preparing the PRP using the standard commercial kits led our research team to look for a simpler, safer, and cheaper technique. After reviewing and testing the main published protocols, we developed a closed system, with a double centrifugation technique, obtaining a satisfactory platelet concentration [ 14 ].…”
Section: Introductionmentioning
confidence: 99%
“…Machado et al . [ 13 ] were able to produce a 4.17-folds increase in platelet concentration in their PRP using this method [ Figure 5 ].…”
Section: Preparation Of Prpmentioning
confidence: 99%
“…Platelet-derived cytokines have been quantified following activation either with calcium alone or calcium/thrombin [27]. High concentrations of platelet-derived growth factor, endothelial growth factor, and transforming growth factor (TGF) were secreted with interleukin (IL)-4, IL-8, IL-13, IL-17, tumor necrosis factor (TNF)-α and interferon (IFN)-α. Unsurprisingly, no cytokines were secreted without platelet activation.…”
Section: Patient and Protocol Differencesmentioning
confidence: 99%