Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus

Ma, Jianping; Wang, Jufang; Liu, Yanfen; Wang, Changyi; Duan, Donghui; Lu, Nanjia; Wang, Kaiyue; Zhang, Lu; Gu, Kaibo; Chen, Sihan; Zhang, Tao; You, Dingyun; Han, Liyuan

doi:10.6061/clinics/2017(02)08

Cited by 29 publications

(25 citation statements)

References 26 publications

(26 reference statements)

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“…40 Although miR-874-3p has not previously been implicated in diabetes, many other miRNAs are involved in diabetes. 45,46 These results support the idea that miR-874-3p may be another diagnostic marker for diabetes, with some specificity for DMED. 42 Moreover, miRNAs are also involved in cardiovascular complications arising from diabetes, 43 especially microcirculation dysfunctions.…”

Section: Discussionsupporting

confidence: 70%

Epigenetic silencing of microRNA‐874‐3p implicates in erectile dysfunction in diabetic rats by activating the Nupr1/Chop‐mediated pathway

Huo

Zhang

et al. 2019

FASEB j.

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Diabetes is a global medical problem that causes many deaths every year.Complications caused by diabetes are serious and affect patients' quality of life.Diabetes mellitus erectile dysfunction (DMED) affects more than half of male diabetes patients. In this study, we determined the role of microRNA-874-3p (miR-874-3p) and nuclear protein-1 (Nupr1) in streptozocin-induced DMED rats. Control rats received equal amount of vehicle. These rats were also injected with lentiviral vector or agomir to silence or overexpress miR-874-3p or Nupr1. Apomorphine (100 μg/kg, s.c.) was used to induce erection and time of erection was recorded. Intracavernosal and mean arterial pressure ratio (ICP/MAP) were also recorded. O 2− level and concentration of thiobarbituric acid reactive substances (TBARs) were detected using lucigenin-derived chemiluminescence method and Colorimetry. Rat cavernosum tissues were collected for subsequent experiments. Cavernosum smooth muscle cells (CSMCs) were also used for in vitro experiments. Nupr1 was found highly expressed (by RT-qPCR and Western blot analysis) in cavernosum tissues from DMED rats.Nupr1 silencing improved the ICP/MAP ratio and erection time. Nupr1 silencing also reduced CSMC apoptosis (by TUNEL assay) as well as decreased O 2− level and TBAR concentration. Nupr1 was targeted and inhibited by miR-874-3p (by luciferase activity and RNA immunoprecipitation assays), which was downregulated in DMED. miR-874-3p downregulation was due to increased methylation at the promoter region (methylation-specific PCR). miR-874-3p overexpression improved erection time and reduced apoptosis. In summary, miR-874-3p was downregulated which led to increased apoptosis and erectile dysfunction in DMED rats, through 1696 | HUO et al. | Establishment of STZ-induced DMED rat modelMale specific pathogen-free (SPF) Sprague-Dawley (SD) rats (n = 90, 8 wk old, 250-300 g) that had normal inhibition of Nupr1-mediated pathway. This study may also provide a new therapeutic direction for the treatment of DMED. K E Y W O R D SChop, diabetic erectile dysfunction, microRNA-874-3p, Nupr1

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Section: Discussionsupporting

confidence: 70%

Epigenetic silencing of microRNA‐874‐3p implicates in erectile dysfunction in diabetic rats by activating the Nupr1/Chop‐mediated pathway

Huo

Zhang

et al. 2019

FASEB j.

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“…In our study, although miR‐320a and miR‐27b did not meet the probability of >.85, the high expression of miR‐320a (1.2‐fold and with a probability of .60) and the decreased expression of miR‐27b (miR‐27b‐5p: −10.9‐fold and with a probability of .81; miR‐27b‐3p: −3.1‐fold and with a probability of .70) were detected (Appendix S3). Using the miRNA‐specific microarray, increased miR‐3939 and miR‐1910‐3p was detected in the serum of the DR patients; however, both miRNAs were not confirmed by PCR in the independent DR and DM groups . In our study, we did not detect the differential expression of the two miRNAs.…”

Section: Discussioncontrasting

confidence: 78%

“…Due to their stability in biofluids such as blood, urine, and aqueous humour (AH), as well as their cell‐type and tissue specificity, miRNAs are potential biomarkers for the detection of DR . A few studies have detected miRNAs in the blood or vitreous humour of DR patients using microarray or polymerase chain reaction (PCR), and suggested that miRNA may be involved in DR and that some miRNAs may be biomarkers for DR . However, miRNA arrays or PCR have only been able to detect some of the mature miRNAs in the known miRbase database .…”

Section: Introductionmentioning

confidence: 99%

“…miRNAs constitute a large family of small (19)(20)(21)(22) nucleotides long), non-coding RNAs that act as post-transcriptional regulators of gene expression. 6 Due to their stability in biofluids such as blood, urine, and aqueous humour (AH), 7 as well as their cell-type and tissue specificity, miRNAs are potential biomarkers for the detection of DR. 4 A few studies have detected miRNAs in the blood or vitreous humour of DR patients using microarray or polymerase chain reaction (PCR), and suggested that miRNA may be involved in DR and that some miRNAs may be biomarkers for DR. [8][9][10][11][12][13] However, miRNA arrays or PCR have only been able to detect some of the mature miRNAs in the known miRbase database. 14,15 No studies have fully detected the entire miRNA profile in AH of DR. Next-generation sequencing (NGS), which not only detects all mature miRNAs, but also predicts novel miRNAs, and have been used to detect the miRNA in AH sample.…”

Section: Introductionmentioning

confidence: 99%

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Landscape of microRNA in the aqueous humour of proliferative diabetic retinopathy as assessed by next‐generation sequencing

Chen

Yuan

Liu

et al. 2019

Clinical Exper Ophthalmology

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Background The microRNAs (miRNA) have been found to play an important role in the pathogenesis of diabetic retinopathy. We try to explore the miRNA and piwi‐interacting RNA (piRNA) profile in the aqueous humour of proliferative diabetic retinopathy (PDR) using next‐generation sequencing (NGS). Methods Aqueous humour samples were collected from nine PDR eyes and nine cataract control eyes, and NGS was performed. Quantitative polymerase chain reaction (qPCR) was used to validate the sequencing results. An oxygen‐induced retinopathy (OIR) model was used to validate the angiogenesis related miRNA. Results In total, 484 miRNAs were differently expressed between the PDR eyes and cataract control eyes, including 210 mature miRNAs and 274 novel miRNAs. Furthermore, eight miRNAs and 30 piRNAs were identified as the most differently expressed between the two groups (P > .85). This differential expression of miRNA was predicted to regulate Rho protein signal transduction, neurotransmitter uptake and histone lysine methylation. Relative expression patterns of miR‐184, ‐150‐5p and ‐93‐5p were confirmed by qPCR. A reduced expression of miR‐93‐5p was confirmed in the OIR model. Conclusions This study comprehensively demonstrated the miRNA and piRNA expression profile of the aqueous humour of PDR eyes, which may serve as a potential biomarker and involved in the pathogenesis of PDR.

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“…Given their high stability and omnipresence in body fluids, several studies have identified the potential of circulating miRNAs as biomarkers of diagnosis, prognosis and management of type 2 diabetes and its vascular complications . Despite this, the profile of circulating miRNAs in DR has been evaluated only recently …”

Section: Introductionmentioning

confidence: 99%

Plasma levels of miR‐29b and miR‐200b in type 2 diabetic retinopathy

Silva

Polina

Crispim

et al. 2018

J Cellular Molecular Medi

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MicroRNAs (miRNAs/miRs) are involved in the pathogenesis of diabetes mellitus and its chronic complications, and their circulating levels have emerged as potential biomarkers for the development and progression of diabetes. However, few studies have examined the expression of miRNAs in diabetic retinopathy (DR) in humans. This case‐control study aimed to investigate whether the plasma levels of miR‐29b and miR‐200b are associated with DR in 186 South Brazilians with type 2 diabetes (91 without DR, 46 with non‐proliferative DR and 49 with proliferative DR). We also included 20 healthy blood donors to determine the miRNA expression in the general population. Plasma levels of miR‐29b and miR‐200b were quantified by reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR). Proliferative DR was inversely associated with plasma levels of miR‐29b (unadjusted OR = 0.694, 95% CI: 0.535‐0.900, P = 0.006) and miR‐200b (unadjusted OR = 0.797, 95% CI: 0.637‐0.997, P = 0.047). However, these associations were lost after controlling for demographic and clinical covariates. In addition, patients with type 2 diabetes had lower miR‐200b levels than blood donors. Our findings reinforce the importance of addressing the role of circulating miRNAs, including miR‐29 and miR‐200b, in DR.

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Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus

Cited by 29 publications

References 26 publications

Epigenetic silencing of microRNA‐874‐3p implicates in erectile dysfunction in diabetic rats by activating the Nupr1/Chop‐mediated pathway

Epigenetic silencing of microRNA‐874‐3p implicates in erectile dysfunction in diabetic rats by activating the Nupr1/Chop‐mediated pathway

Landscape of microRNA in the aqueous humour of proliferative diabetic retinopathy as assessed by next‐generation sequencing

Plasma levels of miR‐29b and miR‐200b in type 2 diabetic retinopathy

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