Analysis of crucial molecules involved in herniated discs and degenerative disc disease

Abstract

OBJECTIVES:Herniated discs and degenerative disc disease are major health problems worldwide. However, their pathogenesis remains obscure. This study aimed to explore the molecular mechanisms of these ailments and to identify underlying therapeutic targets.MATERIAL AND METHODS:Using the GSE23130 microarray datasets downloaded from the Gene Expression Omnibus database, differentially co-expressed genes and links were identified using the differentially co-expressed gene and link method with a false discovery rate <0.25 as a significant threshold. Subsequently, the underlying molecular mechanisms of the differential co-expression of these genes were investigated using Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. In addition, the transcriptional regulatory relationship was also investigated.RESULTS:Through the analysis of the gene expression profiles of different specimens from patients with these diseases, 539 differentially co-expressed genes were identified for these ailments. The ten most significant signaling pathways involving the differentially co-expressed genes were identified by enrichment analysis. Among these pathways, apoptosis and extracellular matrix-receptor interaction pathways have been reported to be related to these diseases. A total of 62 pairs of regulatory relationships between transcription factors and their target genes were identified as critical for the pathogenesis of these diseases.CONCLUSION:The results of our study will help to identify the mechanisms responsible for herniated discs and degenerative disc disease and provides a theoretical basis for further therapeutic study.

DCGL v2.0: An R Package for Unveiling Differential Regulation from Differential Co-expression

“…Three years ago, we released the R package DCGL (referred to as DCGL v1.0 hereafter) [6] , which was designed to identify differentially co-expressed genes and links (DCGs and DCLs, respectively). The DCGL package facilitated the application of our DCEA algorithms DCp and DCe [5] in a diverse array of disease studies [15] – [18] . In our current work, we introduce an upgraded version of DCGL (referred to as DCGL v2.0 hereafter), in which we added ample functions to facilitate differential regulation analyses.…”

Network Pharmacological Dissection of the Mechanisms of Eucommiae Cortex-Achyranthis Radix Combination for Intervertebral Disc Herniation Treatment

“…88,[96][97][98] Akt1 (encoded by AKT1) is a key regulator of the proliferation of IVD cells and its transcript level is associated with IDH progression and deterioration. 50,[99][100][101] Abnormal regulation of matrix metalloproteinase (MMP)-9 activity plays a role in IDH development and progression by inducing disc matrix degradation and collagen loss; it is further related to the prognosis and disease severity in patients with IDH. [102][103][104][105][106][107][108][109] p53 (encoded by TP53) is a mediator of senescence, oxidative stress response, apoptosis, and inflammation in IVD cells and is implicated in neovascularization and infiltration associated with IDH pathogenesis; furthermore, p53 may have potential as a therapeutic target for IDH treatment.…”

“…The IDH-related human targets were retrieved from the databases, including the Therapeutic Target Database, 42 DisGeNET, 43 Online Mendelian Inheritance in Man, 44 GeneCards, 45 Comparative Toxicogenomics Database, 46 and Pharmacogenomics Knowledgebase, 47 as well as from previous relevant literature. 27,[48][49][50][51][52][53][54]…”

Intervertebral disc: regeneration, herniation formation stages and molecular profile (literature review)

“…Важным аспектом в изучении факторов, приводящих к грыжеобразованию, является выяснение Рисунок. Стадии формирования грыжи межпозвонкового диска роли наследственности [7,26]. Единственного «гена дегенерации», приводящего к нарушению структуры межпозвонкового диска, не найдено [5].…”