Transplantation of Adipose Tissue Mesenchymal Stem Cells in Experimental Chronic Chagasic Cardiopathy

Abstract

Thus, it is concluded that administration of intraperitoneal STAT can reduce inflammation and fibrosis in the heart of mice chronically infected with T. cruzi; however, there were no effects on the cardiac function two months after transplantation.

Intramyocardial transplantation of cardiac mesenchymal stem cells reduces myocarditis in a model of chronic Chagas disease cardiomyopathy

“…Inflammatory cell modulation was accompanied by a reduction of TNF-α gene expression, but TGF-β, an anti-inflammatory cytokine, was upregulated. Previous studies have shown similar results with the use of MSCs obtained from adipose tissue and from bone marrow mononuclear cells [3,30]. …”

“…Morphometric analyses were performed 2 months after treatment by using software Image Pro Plus version 7.0 (Media Cybernetics). The number of inflammatory cells was determined by counting 10 fields (400×) per heart in hematoxylin-and-eosin-stained sections, and the percentage of fibrosis was determined in Masson’s trichrome-stained heart sections (200×) as previously described [3]. All of the analyses were blinded.…”

Galectin-3 Knockdown Impairs Survival, Migration, and Immunomodulatory Actions of Mesenchymal Stromal Cells in a Mouse Model of Chagas Disease Cardiomyopathy

“…The ability of transplanted MSC to decrease cardiac inflammation in experimental T. cruzi infected mice was shown before in studies that applied systemic and local delivery routes for cell transplantation [14–16, 35]. Here, we demonstrated that transplanted MSC caused downregulation of inflammatory cytokines directly involved in the disease pathogenesis, such as TNF- α and IFN- γ [13].…”

“…An exacerbated immune response directed against the parasite and to host antigens plays a central role in the pathogenesis of CCC, leading to progressive cardiomyocyte loss, fibrosis, arrhythmia, and loss of ventricular function [13]. Previously, it was demonstrated that transplantation of MSC into mice chronically infected with T. cruzi caused a reduction of myocarditis and modulation of fibrosis [14–16]. Additionally, we have shown that Gal-3 expression is increased in the hearts of chronic chagasic mice and in human samples [17, 18].…”

Mesenchymal Stem Cells — Their Antimicrobial Effects and Their Promising Future Role as Novel Therapies of Infectious Complications in High Risk Patients

“…Injection of the co-cultured cells increased ejection fraction and decreased diastolic and end-systolic volumes [72,78]. Intraperitoneal administration of MSCs into a mouse model of chronic chagasic CMP reduced inflammation and fibrosis in hearts of mice but had no effect on cardiac function as shown by another study involving an experimental animal model [79].…”