Comparative assessment between objective and subjective methods in slides stained by immunohistochemistry

Ribeiro, Fernando de Andrade Quintanilha; Pereira, Celina Siqueira Barbosa; Junchen, Ricardo; Yokomizo, Patrícia Lumi; Fregnani, José Humberto Tavares Guerreiro

doi:10.5935/1808-8694.20130129

Cited by 1 publication

(2 citation statements)

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“…The level of statistical significance for all tests was set at a p ≤ 0.05. Intensity levels of α-DG and β-DG were treated as categorical variables [13] and independently correlated with clinical parameters in both univariate and multivariate analysis of variance. In multivariate analysis of variance, the primary and secondary outcomes were spontaneous rupture of membranes (SROM), labor, and term gestation (>37 weeks' gestational age).…”

Section: Methodsmentioning

confidence: 99%

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Dystroglycan in Human Fetal Membranes Decreases in Later Gestation and with Spontaneous Membrane Rupture

Theiler

Snyder²,

Theiler

2014

Gynecol Obstet Invest

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Background/Aims: The transmembrane protein dystroglycan (DG) is known to anchor the cell membrane to the extracellular matrix, and is susceptible to cleavage by matrix metalloproteinases. This study tested the hypothesis that changes in DG abundance in fetal membranes (FM) occur late in gestation, with spontaneous rupture of membranes (SROM), and during labor. Methods: FM were collected from a prospective cohort consisting of four groups of patients (term labor, term unlabored, preterm labor, and preterm unlabored). FM were subjected to immunohistochemical staining using antibodies specific for α- and β-DG subunits, and staining intensity was graded by a blinded pathologist. Results: α- and β-DG staining was significantly decreased at term and after SROM (p < 0.05), but not in the presence of labor. Conclusions: Decreased DG intensity was seen in FM of patients at term and with SROM, but no change was observed with labor.

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Section: Methodsmentioning

confidence: 99%

“…IHC intensity was scored on a progressive intensity scale of 0-3, with 0 representing undetectable staining and 3 representing abundant staining [13]. Negative control IgG was examined for each specimen.…”

Section: Methodsmentioning

confidence: 99%

Dystroglycan in Human Fetal Membranes Decreases in Later Gestation and with Spontaneous Membrane Rupture

Theiler

Snyder²,

Theiler

2014

Gynecol Obstet Invest

View full text Add to dashboard Cite

show abstract

Comparative assessment between objective and subjective methods in slides stained by immunohistochemistry

Abstract: Our study showed that the irregular characteristics of middle ear cholesteatoma slides stained by immunohistochemistry prevents its proper objective evaluation, while the subjective assessment by experienced examiners was more reliable.

Cited by 1 publication

References 6 publications

Dystroglycan in Human Fetal Membranes Decreases in Later Gestation and with Spontaneous Membrane Rupture

Dystroglycan in Human Fetal Membranes Decreases in Later Gestation and with Spontaneous Membrane Rupture

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