First detection of metallo-β-lactamases in nosocomial isolates of<i>Pseudomonas aeruginosa</i>in Alagoas, Brazil

Barros, Emmily M. L. R.; Morais, Viviane Martha Santos de; Castro, Karina C. B.; Orsi, Alice R.; Maranhão, Fernanda Cristina de Albuquerque; Silva, Zenaldo P.; Silva, Denise M. W.

doi:10.5935/1676-2444.20150047

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…We speculate that the resistance mechanisms of the aztreonam-resistant isolates in this study may involve AmpC-type β-lactamases, Extended Spectrum Beta-Lactamases, and the MexAB-OrpM efflux pump. 7,22,23 The limitations of this study were the small number of isolates and lack of clonal relatedness information. Therefore, repetitive clones might have been present.…”

Section: Discussionmentioning

confidence: 96%

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Prevalence of Imipenemase and Verona Integron-Encoded Metalloβ-Lactamase in Imipenem-Resistant Pseudomonas aeruginosa

Tunyapanit

Pruekprasert

Laoprasopwattana

et al. 2021

J Health Sci Med Res

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Objective: To determine the prevalence of metallo-β-lactamase (MBL) including imipenemase (IMP) and Verona integronencoded metallo-β-lactamase (VIM) in imipenem-resistant Pseudomonas aeruginosa (IRPA) isolates and investigate the in vitro activities of 9 antimicrobial agents against MBL-positive isolates. Material and Methods: Seventy-eight IRPA isolates were obtained from Songklanagarind Hospital. MBL production was detected by the combined-disk test, and the blaIMP and blaVIM genes were determined via Polymerase Chain Reaction (PCR). The in vitro activities of amikacin, gentamicin, aztreonam, ceftazidime, meropenem, ciprofloxacin, colistin, piperacillin/ tazobactam, and ticarcillin/clavulanate were determined using the E-test method. Results: Of the 78 IRPA isolates, 20 (25.6%, 95% CI 17.2-36.4%) were MBL phenotype-positive, and 30 (38.5%, 95% CI 28.5-49.6%) were MBL genotype-positive (29 Imipenemase (IMP)-type and 1 Verona integron-encoded metallo-βlactamase (VIM)-type). Ninety percent of the MBL genotype-positive isolates were MDR. Most MBL genotype- positive isolates were susceptible to colistin (susceptibility rate of 96.7% and MIC50 value of 1.5 µg/mL). Conclusion: Our results showed a high prevalence of MBL-positive isolates (38.5%) in IRPAs, and the IMP-type isolates were dominant among the metallo-β-lactamase-producing imipenem-resistant P. aeruginosa tested. Most MBL-positive isolates were susceptible to colistin and had low MIC50 values.

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Section: Discussionmentioning

confidence: 96%

“…Similarly, the study of Abaza et al 18 demonstrated that 73.3% of their 30 MBLPA isolates were resistant to aztreonam. Furthermore, the study of Barros et al 22 reported the IMP-type MBLPA isolates to be resistant to aztreonam. Notably, we observed that all of our aztreonam-resistant isolates were MDR.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Imipenemase and Verona Integron-Encoded Metalloβ-Lactamase in Imipenem-Resistant Pseudomonas aeruginosa

Tunyapanit

Pruekprasert

Laoprasopwattana

et al. 2021

J Health Sci Med Res

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The epidemiology of carbapenemases in Latin America and the Caribbean

Escandón-Vargas

Reyes

Gutiérrez

et al. 2016

Expert Review of Anti-infective Therapy

103

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Enterobacteriaceae, Pseudomonas spp., and Acinetobacter spp. infections are major causes of morbidity and mortality, especially due to the emergence and spread of β-lactamases. Carbapenemases, which are β-lactamases with the capacity to hydrolyze or inactivate carbapenems, have become a serious concern as they have the largest hydrolytic spectrum and therefore limit the utility of most β-lactam antibiotics. Areas covered: Here, we present an update of the current status of carbapenemases in Latin America and the Caribbean. Expert commentary: The increased frequency of reports on carbapenemases in Latin America and the Caribbean shows that they have successfully spread and have even become endemic in some countries. Countries such as Brazil, Colombia, Argentina, and Mexico account for the majority of these reports. Early suspicion and detection along with implementation of antimicrobial stewardship programs in all healthcare settings are crucial for the control and prevention of carbapenemase-producing bacteria.

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First detection of metallo-β-lactamases in nosocomial isolates ofPseudomonas aeruginosain Alagoas, Brazil

Cited by 2 publications

References 24 publications

Prevalence of Imipenemase and Verona Integron-Encoded Metalloβ-Lactamase in Imipenem-Resistant Pseudomonas aeruginosa

Prevalence of Imipenemase and Verona Integron-Encoded Metalloβ-Lactamase in Imipenem-Resistant Pseudomonas aeruginosa

The epidemiology of carbapenemases in Latin America and the Caribbean

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