PhotoMetrix: An Application for Univariate Calibration and Principal Components Analysis Using Colorimetry on Mobile Devices

Helfer, Gilson Augusto; Magnus, Vinícius Silveira; Böck, Fernanda Carla; Teichmann, Aline; Ferrão, Marco Flôres; Costa, Adilson Ben da

doi:10.5935/0103-5053.20160182

Cited by 50 publications

(56 citation statements)

References 9 publications

(9 reference statements)

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“…From the five samples that had been analyzed, the sample of fried tempe shows 5% and 10% of wax concentration which is indicated on quadrants of K2 and K3 (Figure 3). The test result is in accordance with the research conducted by Helfer and other researchers who provide the ability of Photometrix ® application in grouping banknotes based on their color from different countries based on the paper used (Helfer et al 2017). As a comparative method, TLC test was conducted and it showed that there were spots on the sample of fried tempe with Rf value of 0.52 and 0.50 which is equivalent to Rf value of the sample of cooking oil which is deliberately added wax.…”

Section: Application Methods On Gorengan Samplesupporting

confidence: 87%

“…G channel on Photometrix ® application is selected as measurement channel because it has r value approaching ± 1. This shows relationship between wax concentrations in cooking oil with color intensity that is produced (Helfer et al, 2017). The result of linearity measurement of wax analysis in cooking oil was conducted to seven different wax solutions with 1% until 25% concentration detected by smartphone presented in Figure 1.…”

Section: Results and Discussion The Results Of Selection And Optimizatmentioning

confidence: 94%

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Qualitative Analysis Method of Detection of Wax Content in Gorengan Using Smartphone

Yulia

Wasito

Fatoni

2018

J.Pharm.Sci.Community

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Wax is one of the compounds that can be misused to be added to Gorengan, Indonesian fritter, to keep them crispy. Gorengan containing wax is difficult to identify visually, so a quick and easy method of detecting wax content is required. The purpose of this research is to develop and evaluate the analytical performance of detecting wax content in gorengan using smartphone. Gorengan sample was dissolved with hexane and then added reagent that will give discoloration followed by analysis using smartphone. Some analysis performance parameters were evaluated in terms of linearity and detection limit, qualitative analysis capability, precision, and selectivity test. The developed method was also applied in some gorengan samples. The result shows that the detection of wax content in gorengan can be conducted by using reagent consisting of NaOH, Schift, and curcumin (1 : 2 : 2). Performance analysis shows that the linearity measurement at concentration between 10% and 25% has correlation coefficient (r) of 0.9537 with detection limit at concentration of 2% and precision (%RSD) less than 3%. The developed method can be applied for the detection of wax content in gorengan in the market. ABSTRAKLilin merupakan salah satu bahan yang dapat disalahgunakan untuk ditambahkan pada gorengan agar tetap renyah. Gorengan yang mengandung lilin sulit untuk diidentifikasi secara visual, sehingga diperlukan metode deteksi kandungan lilin yang cepat dan mudah. Tujuan penelitian ini adalah untuk mengembangkan dan menguji kinerja metode analisis kandungan lilin dalam gorengan secara kolorimetri menggunakan smartphone. Penelitian ini merupakan penelitian eksperimental menggunakan gorengan sebagai objek penelitian. Gorengan dilarutkan dengan heksan dan kemudian ditambahkan reagen yang akan memberikan perubahan warna yang selanjutnya dapat dianalisa menggunakan aplikasi smartphone. Beberapa parameter kinerja analisis dievalusi meliputi uji linearitas dan batas deteksi, uji kemampuan analisis secara kualitatif, uji presisi, serta uji selektifitas. Metode yang dikembangkan juga diaplikasikan untuk mendeteksi kandungan lilin pada beberapa sampel gorengan yang dijual di masyarakat. Hasil penelitian menunjukkan bahwa deteksi kandungan lilin dalam gorengan dapat dilakukan dengan menggunakan reagen yang terdiri dari NaOH, Schift, dan kurkumin (1 : 2 : 2). Kinerja analisis metode yang dikembangkan menunjukkan bahwa linearitas pengukuran pada konsentrasi lilin 10% hingga 25% memiliki nilai koefisien korelasi (r) 0,9537 dengan nilai batas deteksi 2% Jurnal Farmasi Sains dan Komunitas, 2018, 15(1), 7-15 8 Yulia et al. dengan nilai presisi (%RSD) kurang dari 3%. Metode yang dikembangkan dapat diaplikasikan untuk deteksi kandungan lilin pada sampel gorengan di pasaran.

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Section: Application Methods On Gorengan Samplesupporting

confidence: 87%

Section: Results and Discussion The Results Of Selection And Optimizatmentioning

confidence: 94%

Qualitative Analysis Method of Detection of Wax Content in Gorengan Using Smartphone

Yulia

Wasito

Fatoni

2018

J.Pharm.Sci.Community

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“…O uso de imagens digitais vem sendo aplicado em análises que eram restritas a equipamentos como colorímetros, espectrofotômetros e fluorômetros. 11 A análise de imagens digitais associada à análise multivariada permite o desenvolvimento de procedimentos simples, rápidos, não destrutivos e de baixo custo. O uso dessas imagens obtidas por scanners, câmeras digitais e outros, fornece resultados tão precisos quanto os instrumentos associados com análise multivariada.…”

Section: Artigounclassified

“…Diversos estudos, utilizando o aplicativo PhotoMetrix como ferramenta de análises, vem sendo realizado como a caracterização de tanino por PCA demonstrando o desenvolvimento de um método rápido, não destrutivo e de baixo custo. 12 Entre as aplicações reportadas estão: (i) quantificação de etanol em cachaça, 16 (ii) quantificação de ferro em suplementos vitamínicos e aplicabilidade em cédulas de Real, podendo diferenciá-las de cédulas de Peso argentino de maneira eficaz por PCA, 11 (iii) análise indireta de iodo no biodiesel, 17 (iv) estabilidade térmica do leite, 18 (v) identificação de taninos em amostras comerciais 12 e (vi) monitoramento de flúor em água. 19 Assim, visto a crescente incidência de casos de adulteração e fraudes em documentos, e a necessidade de métodos que exijam pouca manutenção, fácil operação, mínimo preparo de amostra, baixo custo, entre outros fatores, este trabalho objetiva avaliar uma nova metodologia para análise de autenticidade de cédulas de reais de R$10, 20, 50 e 100, buscando alcançar essas necessidades por meios de métodos quimiométricos aliados à análise de imagem digital.…”

Section: Artigounclassified

Classificando Cédulas Brasileiras (R$) Usando Análise De Imagem Por Smartphone

Vittorazzi¹,

Costa²,

Coelho³

et al. 2020

Quim. Nova

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A new method to identify the authenticity of Brazilian banknotes (R$) is proposed using digital image analysis by a smartphone application, Photometrix ® , associated with chemometric tools. Authentic banknotes (n = 4) obtained in local bank and falsified (n = 12) by the author himself by means of three printers (laser-Deskjet ® , wax-Color Qube ® and inkjet-Deskjet ®) were used for the development of the methodology. An experimental planning was performed to evaluate the authenticity of the banknotes determining: the best region (holographic band, tactile region in the lower left and right upper number, effigy microimpressions, lower number left, animal figure, watermark and puzzle); Region of Interest (ROI) (16x16; 32x32 and 64x64 pixels), focal length (8, 12, 15 and 20 cm) and variation of the smartphone (Motorola G5 Plus, Samsung S7 and LG K10). The best regions chosen were effigy microimpressions and round the animal. Promising results were obtained from ROI 16x16 pixels with focal length fixed at 10 cm. Thus, the application presents promising features for analyzing the authenticity of banknotes by digital images making it fast, non-destructive, portable, with low cost, and can be accessed by all society.

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“…In this context, we used a free software app called PhotoMetrix [15] to estimate the enzymatic activity of endoglucanase enzymes. PhotoMetrix has been used for efficiently helping to determine iron in vitamin supplement tablets [16], iodine in biodiesel [17], ethanol in sugarcane spirit samples [18], and for monitoring of acid-base titrations on paper platforms [19], estimation of pH in milk samples [20] and chromium speciation in leather samples [21].…”

Section: Introductionmentioning

confidence: 99%

Easy Estimation of Endoglucanase Activity Using a Free Software App for Mobile Devices

Pereira¹,

Guedes²,

Lucena³

et al. 2020

Br J Anal Chem

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Imaging-PLS was applied for cellulase enzymes from Aspergillus niger and Trichoderma reesei This study describes the estimation of the enzymatic activity of endoglucanase based on the 3,5-dinitrosalicylic acid (DNS) method and carboxymethylcellulose (CMC) substrate using glucose as the analyte. As an alternative to measuring the colored product obtained by the reaction between glucose and DNS, a free software app for mobile devices was used for estimating the color intensity of solutions, replacing the need of a UV-Vis spectrophotometer (reference method). The app is able to convert images in color histograms to red (R), green (G) and blue (B) scales and to provide univariate and multivariate calculations of the image data. The chemometric technique partial least squares (PLS) was employed with the app and presented accurate results that were comparable to those of the reference method. A correlation coefficient of 0.983 was obtained for the linear range between 0.60 and 2.60 mg mL-1 of glucose, and the standard error of cross-validation (SECV) was 0.219 mg mL-1. The proposed method (imaging-PLS) was applied for samples of cellulase enzymes from Aspergillus niger and Trichoderma reesei, revealing an interesting alternative to estimate the enzymatic activity of endoglucanase.

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PhotoMetrix: An Application for Univariate Calibration and Principal Components Analysis Using Colorimetry on Mobile Devices

Cited by 50 publications

References 9 publications

Qualitative Analysis Method of Detection of Wax Content in Gorengan Using Smartphone

Qualitative Analysis Method of Detection of Wax Content in Gorengan Using Smartphone

Classificando Cédulas Brasileiras (R$) Usando Análise De Imagem Por Smartphone

Easy Estimation of Endoglucanase Activity Using a Free Software App for Mobile Devices

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