THE PHENOLIC COMPOSITION OF THE HEPATOPROTECTIVE AND ANTIOXIDANT FRACTIONS OFAlbizia lebbeckL.

Abstract

An investigation of the hepatoprotective and antioxidant activities of the chloroform, ethyl acetate and n-butanol fractions of the leaves of Albizia lebbeck L. was performed. The first two fractions expressed the best results regarding the suppression of the increased levels of plasma amino-transferases and alkaline phosphatase in liver-damaged mice (after intoxication with CCl 4 ) compared with silymarin and the significantly increased GSH content of alloxan-induced diabetic rats compared with vitamin E (tests and reference drugs were orally administered). The bioactive fractions of Albizia lebbeck L. were subjected to chromatographic analysis to investigate their phenolic contents using a HPLC-PDA-ESI-MS/MS technique in the negative ion mode. The results constitute the first report of the presence of seven compounds in the genus Albizia, three of which were identified as 3-O-caffeoylquinic acid, cafeic acid, myricetin; four other flavonoids (in mg 100 g -1 dry powder ± SD), myricetin 3-O-rhamnoside (0.129 ± 0.0052), quercetin 3-O-dideoxypentoside (0.011 ± 0.001), kaempferol 3-O-glucoside (0.015 ± 0.002), quercetin 3-O-dihexoside (0.138 ± 0.002); quercetin 3-O-rutinoside at a level of 0.135 ± 0.004; and the aglycones quercetin, luteolin and kaempferol. Method validation was performed, providing an analytical technique that can be used to detect trace amounts of the identified compounds in Albizia extracts with rapid sample preparation.

Chemical Composition, Toxicity, Antinociceptive, and Anti-Inflammatory Activity of Dry Aqueous Extract of Varronia multispicata (Cham.) Borhidi (Cordiaceae) Leaves

“…Flavonoids, quercetin, and kaempferol, as well as their glycosides are widespread in the plant kingdom. Nevertheless, they have a variety of biological activities such as hepatoprotective, antioxidant, and anti-inflammatory (Mohamed et al, 2010; Costa et al, 2012; Sokkar et al, 2016). Flavones are part of another subgroup of flavonoids that are widely studied because they have various pharmacological activities, suggesting that the presence of these flavonoids may contribute to the antinociceptive and anti-inflammatory properties of AEVm found in this study.…”

“…These properties may be related to flavonoids present in the species (Mohamed et al, 2010; Costa et al, 2012; Sokkar et al, 2016). In addition, antioxidant effect of AEVm may be involved in preserving the histological and functional normality of the liver, an organ with intense oxidative metabolism (Sokkar et al, 2016). Results presented highlight not only the safety of acute oral use of the extract, but also its possible protective effects.…”

Saponins from the Pericarp of Albizia lebbeck (Benth) L. and Their Biological Screening on Laboratory Animals

“…In continuation to our studies on A. lebbeck tree [9,10], the biological activity was performed on the 50% ethanol extract and its fractions of the pericarp. A detailed phytochemical investigation of the highly potent saponins in n-butanol fraction pod was presented here; structures were based mainly on NMR and LC-MSn (n=2).…”

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“…Albizia species has a record of use in folkloric medicine, particularly for asthma and allergic disorders and, also practiced in Ayurveda for the treatment of bronchitis, asthma, leprosy, eczema, pruritus, paralysis 8 and has been reported to retain antibacterial, and antioxidant, anti-microbial, Anticancer 9, 16, analgesic anti-inflammatory, and antipyretic 17 and also anti-allergic activities 18 . The therapeutic activity against Parkinson's disease also has been published 19 . The medicinal plants of this species have been reported for hepatoprotective and antioxidant, acetylcholinesterase inhibitory 20, 21, anti-diabetic 22, ant-idiarrheal, antispasmodic, and Bronchodilator effects 23 .…”