PHYTOCHEMICAL STUDY ON ANTIMICROBIAL AND CYTOTOXIC ACTIVITY OF SPECIMENS OF<i>Leonotis nepetifolia</i>L. R. (Br)

Oliveira, Ana Paula de; Guimarães, Amanda Leite; Pacheco, Alessandra Gomes Marques; Araújo, Camila de Souza; Júnior, Raimundo G. Oliveira; Lavor, Érica Martins de; Silva, Mariana G.; Araújo, Edigênia C. C.; Mendes, Rosemairy Luciane; Rolim, Larissa Araújo; Costa, M. F.; Farias, Henrique C. L.; Pessoa, Cláudia; Lopes, Norberto P.; Marques, Lucas Maciel Mauriz; Almeida, Jackson R.G.S.

doi:10.5935/0100-4042.20150160

Cited by 2 publications

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Leonotis nepetifolia (Family Lamiaceae) with pantropical distribution is known in Brazil as “Cordão de São Francisco” (Cruz et al, 2011). Previous studies carried out with extracts of this species revealed the cytotoxic potential against human tumor cell lines MCF7 (breast), Hep2 (human epithelial type 2), SF295 (human brain), OVCAR8 (ovarian) and HCT116 (human colorectal) (Veerabadran et al, 2013; Oliveira et al, 2016). A previous study carried out by our research group provided the identification that the flavonoid cirsiliol is one of the compounds responsible for the cytotoxic activity observed in this plant (Oliveira et al, 2016).…”

Section: Introductionmentioning

confidence: 91%

Influence of light intensity, fertilizing and season on the cirsiliol content, a chemical marker ofLeonotis nepetifolia(Lamiaceae)

Oliveira

Borges

Pereira

et al. 2019

PeerJ

View full text Add to dashboard Cite

BackgroundLeonotis nepetifolia (Family Lamiaceae) is a medicinal plant from which the flavonoid cirsiliol with sedative, hypnotic, anti-inflammatory and cytotoxic activity has been extracted.MethodsSeedlings were cultivated under different levels of shade in native or fertilized modes. The content of cirsiliol was measured monthly by high-performance liquid chromatography and the total phenolic content by the Folin-Ciocalteu method. Monitoring of growth was carried out with the weekly measurement of height until the stabilization of growth.ResultsThe application of fertilizing and/or shading does not alter significantly the cirsiliol content. However, this content varies throughout the year, reaching the peak production in the summer, independently of the treatment applied. This same profile, with production in the summer, was also verified for phenolic compounds, reaching 58.15 ± 9.35 mg of equivalents of gallic acid per g of extract in the summer, content 1.84 times greater than the content verified in winter (31.56 ± 4.09 mg of gallic acid/g of extract). Although shading and fertilizing had no effect on cirsiliol content, the results also showed a positive influence on the height and biomass of the plant, which can causes a higher yield of extractable material.DiscussionBiotic and abiotic stresses are able to increase or decrease the production of secondary metabolites, including phenolic compounds in medicinal plants and, as the stress response is peculiar to each species, cultivation studies become necessary. The present study reports by the first time the influence of shading, fertilizing and seasons in cirsiliol content in L. nepetifolia. Among analyzed variables, the seasons showed a larger influence in expression of cirsiliol and among seasons, our results showed that the summer is the ideal season for collections. In summer, the photoperiod is larger than in other seasons of the year and due to that, the plants need greater protection against the long photoperiod. For this, the plants increase the production of phenolic compounds as observed in this study. Although they do not influence the production of cirsiliol, the shading and nutrients in soil favor growth and leaf area of several plants, explaining, thus, the higher height and biomass obtained.

show abstract

Section: Introductionmentioning

confidence: 91%

Influence of light intensity, fertilizing and season on the cirsiliol content, a chemical marker ofLeonotis nepetifolia(Lamiaceae)

Oliveira

Borges

Pereira

et al. 2019

PeerJ

View full text Add to dashboard Cite

show abstract

Cytotoxic Activity of Chemical Constituents and Essential Oil from the Leaves of Leonotis nepetifolia (Lamiaceae)

Damasceno¹,

Silva²,

Santos³

et al. 2019

Rev. Virtual Quim.

View full text Add to dashboard Cite

Atividade Citotóxica de Constituintes Químicos e Óleo Essencial das Folhas de Leonotis nepetifolia (Lamiaceae) Resumo: O câncer é um problema de saúde que afeta grande parte da população mundial. Nesse contexto, vários grupos de pesquisa têm investigado moléculas com maior eficiência e menores efeitos colaterais. A espécie Leonotis nepetifolia é um arbusto pertencente à família Lamiaceae com atividade citotóxica relatada na literatura. Este trabalho descreve o isolamento dos compostos hentriacontano, palmitato de fitila, estigmasterol glicosídeo, 6,7-dimetoxi-5,3',4'-trihidroxiflavona, apigenina-7-O-glicosídeo e luteolina-7-O-glicosídeo a partir de extratos e a composição química do óleo essencial das folhas de espécimes brasileiras de L. nepetifolia, além das suas atividades citotóxicas in vitro. Os compostos foram identificados por uma série de métodos espectroscópicos e espectrométricos, principalmente RMN (1D e 2D) e CG-EM, bem como por comparação com dados da literatura. A atividade citotóxica de compostos isolados e óleo essencial foi realizada utilizando o ensaio de brometo de 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazólio (MTT), contra linhagens de células tumorais HCT-116 (cólon humano) e SF-295 (glioblastoma). O álcool 1-octen-3-ol foi o composto majoritário do óleo essencial e os compostos hentriacontano, palmitato de fitila, estigmasterol glicosídeo, apigenina-7-O-glicosídeo e luteolina-7-O-glicosídeo foram descritos pela primeira vez nesta espécie. Todos os compostos testados e o óleo essencial apresentaram baixa atividade citotóxica para as linhagens celulares testadas, sugerindo que outros estudos fitoquímicos devam ser conduzidos para a descoberta de outros compostos responsáveis pela atividade citotóxica da espécie.

show abstract

PHYTOCHEMICAL STUDY ON ANTIMICROBIAL AND CYTOTOXIC ACTIVITY OF SPECIMENS OFLeonotis nepetifoliaL. R. (Br)

Cited by 2 publications

References 1 publication

Influence of light intensity, fertilizing and season on the cirsiliol content, a chemical marker ofLeonotis nepetifolia(Lamiaceae)

Influence of light intensity, fertilizing and season on the cirsiliol content, a chemical marker ofLeonotis nepetifolia(Lamiaceae)

Cytotoxic Activity of Chemical Constituents and Essential Oil from the Leaves of Leonotis nepetifolia (Lamiaceae)

Contact Info

Product

Resources

About